Serological and bacteriological responses of water buffalo (Bubalus bubalis) vaccinated with two doses of Brucella abortus strain RB51 vaccine

Abstract

Thirty-two water buffalo (Bubalus bubalis) calves aged 6–10 months were used to evaluate serological responses to Brucella abortus strain RB51 (RB51) vaccination in a dose-response study and to compare the use of two selective media for the isolation of RB51. The animals were randomly divided into three treatment groups. Groups I-III received the recommended vaccine dose (RD) twice 4 weeks apart, RD twice 18 weeks apart and saline once, respectively. Lymph nodes were excised from the three groups and subjected to bacteriological examination to determine the frequency of detection of RB51. Pre- and post-vaccination blood samples were collected and tested for B. abortus antibodies using the buffered plate agglutination test (BPAT), complement fixation test (CFT), and dot-blot assay. Sera taken at all post-inoculation weeks (PIW) were negative for field strain B. abortus using the BPAT. Antibody responses to RB51 were demonstrated in all vaccinates but not in controls by CFT and dot-blot assay from 1 PIW up to 16 weeks following booster vaccination. The agreement for both assays was 80.7% and there was a linear interdependence with a Pearson's correlation coefficient value of 0.578. The frequency of isolation of RB51 from the two selective media used was not significantly different (P > 0.05).

Brucella abortus Strain RB51 Administered to Prepubescent Water Buffaloes, from Vaccination to Lactation: Kinetics of Antibody Response and Vaccine Safety

“…The few studies carried out on the use of the RB51 vaccine in water buffaloes show how the age of administration, the time interval between the first vaccine, a possible booster, and the vaccine dose used, may result in different immune response trends. This makes it is difficult to compare the studies with each other [ 25 , 33 ]. This is therefore important to underline that the data obtained in the present study must be interpreted and correlated with the vaccination scheme described.…”

Experimental Study on Brucella abortus Strain RB51 Vaccinated Water Buffalo (Bubalus bubalis) Challenged with Virulent B. abortus Strain during Pregnancy

“…The experimental groups, vaccination regimen, and the facilities where the study was conducted on water buffalo calves aged 7 -10 months were earlier described [26]. Briefly, the calves were randomly divided into three groups with stratification according to size.…”

Brucellosis in water buffaloes

“…Of these, the most promising is the competitive ELISA (Mathias et al 1998, Molnár et al 2002, Paulin et al 2012 and the FPA (Montagnaro et al 2008, Paulin et al 2012. Dot--Blot assays and CFT are also efficient for detecting significant titers in buffaloes vaccinated with two doses of RB51, being an alternative in serological surveillance for early detection of infected animals (Ramnanan et al 2012).…”