Serological and genetic characterization of Flavobacterium psychrophilum isolated from farmed salmonids in Turkey

Abstract

Turkey was the largest rainbow trout producer of the European countries in 2016, and the reason for this production is mainly attributed to its egg and fry production. Flavobacterium psychrophilum cause the highest rates of mortality in the starting to feeding stages of the fish. In the present study, twenty‐five F. psychrophilum isolates recovered from rainbow trout, coruh trout and brook trout were analysed by RAPD‐PCR, ERIC‐PCR, REP‐PCR and PCR‐RFLP, including 16S rRNA, gyrA and gyrB gene regions and PCR‐based serotyping method. The PCR‐based molecular analysis showed that the isolates could not be differentiated exactly according to isolation source and geographical region. Most isolates were of type‐1 and type‐2, and some of them were of type‐0 and type‐3; in addition, one isolate showed a unique serotype. The combined analysis results showed that F. psychrophilum isolates discriminated as five different genotypes and all isolates were successfully discriminated based on host.

Pseudomonas piscium sp. nov., Pseudomonas pisciculturae sp. nov., Pseudomonas mucoides sp. nov. and Pseudomonas neuropathica sp. nov. isolated from rainbow trout

“…Despite its resistance to disease, tolerance of variable water quality and stress factors, diseases can take a toll on production. As is the case in other countries, several major bacterial fish pathogens, such as Aeromonas , Vibrio , Flavobacterium , Streptococcus and Pseudomonas cause mass death in rainbow trout in Turkey, and this eventually decreases the production of this fish [1–3]. Therefore, information on both Pseudomonas species prevalence in aquaculture farms and how to accurately identify them is of crucial interest.…”

Phenotypic and Genetic Characterization of Flavobacterium psychrophilum Recovered from Diseased Salmonids in China

“…More recently, a PCR-based method was developed for the determination of previously described F. psychrophilum serotypes, increasing the reproducibility of “serotyping” data ( 19 ). Subsequent studies using this molecular serotyping scheme have provided further evidence for various degrees of host-molecular serotype associations ( 20 , 21 ).…”

Host‐specific preference of some Flavobacteriumpsychrophilum multilocus sequence typing genotypes determines their ability to cause bacterial coldwater disease in coho salmon (Oncorhynchuskisutch)

“…type 0) or multiple fish species (e.g. type 2 and type 4; Avendaño‐Herrera et al., 2020; Rochat et al., 2017; Saticioglu et al., 2018; Sundell et al., 2019). In agreement with these studies, isolates US19‐COS, originally recovered from coho salmon (Van Vliet et al., 2016), and US53‐RBT, originally recovered from rainbow trout (Van Vliet et al., 2016), were found to belong to type 0 and type 2, respectively.…”