Serological and genomic characterization of L338, a novel equine group A rotavirus G serotype

Browning, Glenn F.; Chalmers, Rachel M.; Fitzgerald, T. A.; Snodgrass, D. R.

doi:10.1099/0022-1317-72-5-1059

Cited by 92 publications

(57 citation statements)

References 34 publications

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“…G6 and G10 rotaviruses are the two major types isolated from cattle but have been infrequently encountered in humans. Until now, no rotavirus strains of G7 (chicken and cattle), G11 (pigs), G13 and G14 (horses), and G15 (cattle) have been isolated from humans (9,10,48). Only a few epidemiological studies have studied the occurrence of P types.…”

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confidence: 99%

Genetic Characterization of a Novel, Naturally Occurring Recombinant Human G6P[6] Rotavirus

et al. 2003

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A binary classification system has been established for group A rotaviruses, with the viral capsid protein VP7 defining G types and VP4 defining P types. At least 15 G types and 21 P types have been isolated globally with various G and P combinations. Most of the currently circulating human rotaviruses belong to G1P[8], G2P[4], G3P[8], and G4P[8]. We report a human rotavirus strain (B1711) with a novel genotypic VP7/VP4 combination of G6P[6]. This unique rotavirus was isolated from a 13-month-old human immunodeficiency virus (HIV)-negative child of an HIV-seropositive Malian mother that was hospitalized with severe diarrhea in Belgium after returning from a trip to Mali. The VP7 and VP4 genes of the rotavirus strain were sequenced, and phylogenetic trees were constructed. Nucleotide and amino acid sequence comparisons with 15 known G genotypes indicated that the VP7 sequence of strain B1711 was most closely related to an American (Se584) and an Italian (PA151) human G6 strain (95 to 96% nucleotide and 98% amino acid identity). Comparison of the VP4 sequence with 21 P types showed the closest similarity to P[6] genotypes, with greatest similarity to a G8P[6] Malawi strain (mw131) (97% nucleotide and 98% amino acid identity). The B1711 strain is the first reported rotavirus isolate with a G6P[6] genotypic combination. The discovery and surveillance of novel human and nonhuman rotavirus G or P types or of novel G/P combinations is essential for the design of future rotavirus vaccines and for our understanding of rotavirus diversity and evolution.Group A rotaviruses are the single most important etiological agent associated with gastroenteritis in infants and young children (2, 40). Rotavirus-associated diarrhea leads to more than 125 million cases of infantile gastroenteritis and 870,000 deaths each year, primarily in less developed countries (54). In the United States it also results in $274 million in medical care costs and a total of $1 billion in societal costs (including indirect cost of lost parental work time) per year (47). Rotaviruses contain 11 segments of double-stranded RNA within a core shell and are members of the Reoviridae family. Each segment encodes a single viral polypeptide, for a total of five nonstructural and six structural proteins (17). The two outer capsid proteins VP7 and VP4, which independently elicit neutralizing antibodies, are the basis of a binary classification system for rotaviruses: G types (derived from the VP7 glycoprotein) and P types (derived from the protease sensitive VP4 protein). Thus far, 15 different G genotypes and 21 different P genotypes have been reported (2,26,31,48). Because VP4 and VP7 are encoded by different RNA segments, various combinations of G and P types can be observed (40). Most G genotypes were serologically confirmed as serotypes (25). Due to the lack of appropriate antibody reagents, a dual P-typing system (P serotype and P genotype) has been used (18). Strains sharing more than 89% sequence identity at the amino acid level are considered to belong to...

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confidence: 99%

Genetic Characterization of a Novel, Naturally Occurring Recombinant Human G6P[6] Rotavirus

et al. 2003

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“…While the P genotypes of rotavirus from human and other some animal species as bovine and porcine especially had been classified into 35 P types, equine rotaviruses are predominantly in P [12] genotype. At this date, at least six different P types (P [12], P[3], P [11], P [1], P [18] and P [7]) of equine rotaviruses has been reported 4, 13,17,18,29 . The known binary combinations in group A equine rotaviruses are especially G14P [12] 14 and G3P [12] 14 , followed P [1] with combination G10 and G8, P [7] with combination G13 17,20 .…”

Section: Discussionmentioning

confidence: 99%

“…9,14,17,28 while the other G genotypes (G5, G8, G10, G13) from foals were detected sporadically 4,13,[17][18][19] . While the P genotypes of rotavirus from human and other some animal species as bovine and porcine especially had been classified into 35 P types, equine rotaviruses are predominantly in P [12] genotype.…”

Section: Discussionmentioning

confidence: 99%

“…Reports reveals that the majority of rotavirus strain from foals are either G3P [12] or G14P [12] 5,7,9, [11][12][13][14]16,17 . The other strains detected in foals belonging to genotype G5, G8, G10, G13 and P [18] . But there is no report on the G and P genotypes of equine rotavirus in Turkey.…”

Section: Makale Kodu (Article Code): Kvfd-2012-8186mentioning

confidence: 99%

“…For G typing, primers for G3, G13, G14 types 14 and End9 23 were used 14 . For P typing, the second round PCR was performed with primer Con3 24 and primer specific for genotypes P [12] and P [18] 15 . Additionally, the expected size amplicons were produced for NSP4 gene region from all samples positive for rotavirus with primers and protocols elsewhere 25 .…”

Section: Rna Extraction Rt-pcr and Genotyping Of Ervmentioning

confidence: 99%

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Rotavirus Diarrhea Outbreaks in Arabian Thoroughbred Foals in a Stud Farm, Turkey

Alkan

Timurkan²,

Karayel³

2013

Kafkas Univ Vet Fak Derg

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SummaryIn this study, the aetiological agent of diarrhea in Arabian thoroughbred foals housed in a stud farms and it's molecular characterization were reported. Out of sampled seven foals with diarrhoea, five were detected positive by RT-PCR depending on the amplification of the VP6 gene of rotavirus. Then the aetiological agent was characterized genetically by sequence analysis of the genome segments encoding VP6, VP7, VP4 and NSP4 of rotavirus. Findings revealed that the Turkish equine rotavirus circulating within this stud farm belongs to G3 and P[12] genotype with E2 NSP4 and I6VP6. This is the first study to report the G and P genotypes of equine group A rotaviruses in Turkey. Keywords: Rotavirus, Equine, Genotyping, Diarrhoea, Turkey Türkiye'de Bir Arap Atı İşletmesindeki Taylarda Rotavirus İshali Salgını ÖzetBu çalışmada, bir at yetiştiriciliği işletmesinde bulunan safkan arap taylarındaki ishal olgusunun etiyolojik ajanının tespiti ve moleküler karakterizasyonu bildirildi. İshal semptomlu yedi taydan sağlanan materyallerin beşinde RT-PCR tekniği ile rotavirus VP6 geni yönünden pozitiflik saptandı. Daha sonra enfeksiyona neden olan virusun VP6, VP7, VP4 ve NSP4 gen bölgelerini kodlayan gen bölgelerinin dizi analizi yapıldı. Elde edilen verilere dayanılarak söz konusu at yetiştiriciliği işletmesinde enfeksiyona neden olan ERV saha suşunun E2 NSP4 ve I6VP6 ile ilişkili G3P[12] genotipinde olduğu belirlendi. Bu çalışma, Türkiye'de atlardaki grup A rotavirusların G ve P genotiplerinin bildirildiği ilk çalışmadır.

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Application of Organ Culture of Small Intestine to the Investigation of Enterocyte Damage by Equine Rotavirus

Batt,

Embaye,

van de Waal

et al. 1995

J. pediatr. gastroenterol. nutr.

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SummaryWe used organ culture of jejunal mucosal explants obtained from ponies aged between 2 and 12 months to study enterocyte damage by group A strains of equine rotavirus. Electron microscopy of jejunal explants maintained for 48 h in the presence of organ culture medium alone showed that entcrocytes were structurally intact and had a densely packed brush border and overlying mucus. Similarly, examination of explants maintained in the presence of rotavirus for 48 h revealed no apparent ultrastructural abnormalities. However, obvious replication and assembly of virus in enterocytes had occurred following exposure to equine rotavirus strains H2 (three of eight occasions), trypsinactivaled H2 (two of four), L338 (five of five), and EQ124 (two of two) although brush borders and intracellular organelles ap peared unaltered. Despite the absence of obvious ultra structural damage, analytical subcellular fractionatior showed that rotavirus infection can result in decreasec activities of microvillar membrane enzymes, with a par ticular loss of the main brush border peak of modal den sity 1.21 g/ml. These findings demonstrate that organ cul ture of small intestine may be applied to the investigatior of rotavirus pathogenicity; they further suggest that cer tain strains impair enterocyte function by affecting the turnover of microvillar membrane proteins.

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Serological and genomic characterization of L338, a novel equine group A rotavirus G serotype

Cited by 92 publications

References 34 publications

Genetic Characterization of a Novel, Naturally Occurring Recombinant Human G6P[6] Rotavirus

Genetic Characterization of a Novel, Naturally Occurring Recombinant Human G6P[6] Rotavirus

Rotavirus Diarrhea Outbreaks in Arabian Thoroughbred Foals in a Stud Farm, Turkey

Application of Organ Culture of Small Intestine to the Investigation of Enterocyte Damage by Equine Rotavirus

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