2020
DOI: 10.36103/ijas.v51i4.1100
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Serological and Molecular Diagnosis of Toxoplasma Gondii Among Ewes and Horses in Duhok Province-Iraq

Abstract: This study was aimed to demonstrate the seroprevalence and molecular detection of Toxoplasma gondii among ewes and horses as well as to determine the risk factors for infection in Duhok province. Sera and blood samples from 700 ewes and 62 horses were collected. Sera were examined by indirect ELISA for detection of anti-T. gondii IgM antibodies and in molecular study, DNA was extracted, then by PCR, B1 gene was amplified and the product visualized and sent off for sequencing. Serologically, the prevalence of t… Show more

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“…By molecular findings using PCR, the prevalence of toxoplasmosis among sheep was revealed to be 31.3% in Sulaymaniyah province (northern region of Iraq); similarly, toxoplasmosis has been reported from sheep with various prevalence rates: 21.7% (Mikaeel and Al-Saeed, 2020 ), a low prevalence of 8.75%, and 9% was reported from other regions of Iraq by Al-Shaibani et al ( 2019 ) and Al-Abodi ( 2021 ), respectively.…”
Section: Discussionmentioning
confidence: 83%
“…By molecular findings using PCR, the prevalence of toxoplasmosis among sheep was revealed to be 31.3% in Sulaymaniyah province (northern region of Iraq); similarly, toxoplasmosis has been reported from sheep with various prevalence rates: 21.7% (Mikaeel and Al-Saeed, 2020 ), a low prevalence of 8.75%, and 9% was reported from other regions of Iraq by Al-Shaibani et al ( 2019 ) and Al-Abodi ( 2021 ), respectively.…”
Section: Discussionmentioning
confidence: 83%