Serological and molecular investigation of Newcastle disease in household chicken flocks and associated markets in Eastern Shewa zone, Ethiopia

Chaka, Hassen; Goutard, Flavie; Gil, Patricia; Abolnik, Célia; Almeida, Renata Servan de; Bisschop, Shahn P.R.; Thompson, Peter N.

doi:10.1007/s11250-012-0278-y

Cited by 37 publications

(39 citation statements)

References 42 publications

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“…In total, 8.4% of the swab samples were positive for NDV of which 38.5% were virulent strains. The finding is in agreement with earlier studies which revealed the circulation of virulent strains of NDV in village chickens in Ethiopia [18,19]. The first documented evidence of ND in Ethiopia dates back to 1978 when an outbreak occurred in Eritrea, the then, Northern part of the country.…”

Section: Discussionsupporting

confidence: 92%

Serological and Molecular Study of Newcastle Disease Virus in Village Chickens in Selected Rift-Valley Areas, Ethiopia

Terefe¹,

Belaineh²,

Chaka³

2015

J Veterinar Sci Technol

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Newcastle Disease (ND) is a serious and commonly fatal viral poultry disease, which is widely distributed throughout the world. In most developing countries including Ethiopia, ND is the most important disease causing huge economic losses. A cross sectional study was conducted in February 2014 in village chickens at three selected districts in and around rift valley lakes of Ethiopia to determine the prevalence of Newcastle disease virus (NDV) by serological and molecular methods. A total of 155 sera and 155 swab samples were collected from village chickens in Bishoftu, Tikur wuha and Ziway districts. The sera samples were analyzed by Competitive-ELISA. ND viruses were isolated from the tracheal swab specimens, through inoculation in embryonated chicken eggs and were characterized genetically by using molecular methods. Real-time RT-PCR targeting a conserved region of the M gene was employed to amplify all APMV1. M-gene positive samples were further analyzed by Real-time RT-PCR targeting F-gene to specifically amplify the virulent strains. The overall sero prevalence of ND was 11.6% with no statistically significant differences between the study districts at 95% confidence level. Thirteen isolates were positive for APMV1 out of which 38.4% (5/13) were virulent NDV strains. This study provides important information on serological and virological profile of NDV and highlights the importance of continuous surveillances to better understand the epidemiology of the disease.

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Section: Discussionsupporting

confidence: 92%

Serological and Molecular Study of Newcastle Disease Virus in Village Chickens in Selected Rift-Valley Areas, Ethiopia

Terefe¹,

Belaineh²,

Chaka³

2015

J Veterinar Sci Technol

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“…1999;Alexander and Senne, 2008). Also, its inestimable impact on food security is a course for concern due to the reduction in dietary protein supply mostly in developing countries (Aboe et al, 2006;Saidu et al, 2006;Olabode et al, 2008;Chaka et al, 2012;Solomon et al, 2012).…”

Section: Introductionmentioning

confidence: 99%

Comparative Haematology of Chickens and Turkeys Experimentally Infected with a Velogenic Newcastle Disease Virus

Okoroafor¹,

Okorie-Kanu²,

Animoke³

et al. 2019

Nig. Vet. J.

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This study compared the haematological findings in cockerels and turkeys infected with a velogenic Newcastle disease virus. One hundred and twenty day-old birds each of cockerels and turkeys, making a total of two hundred and forty were used for the study. The bird types were randomly divided into four groups each making a total of eight groups. Two groups in each species were vaccinated against NDV with La Sota vaccine at three weeks of age via drinking water. The vaccinated and unvaccinated cockerels and turkeys were inoculated with the velogenic NDV at six weeks of age while the control groups were not vaccinated and not inoculated. Blood samples were randomly collected from five birds in each group for haematological analyses at days 0, 3, 6, 10, 15 and 21 post inoculation (pi). The results showed significant (p < 0.05) reductions in packed cell volume, haemoglobin concentration and total red blood cell count in unvaccinated infected turkeys, but showed significant (p < 0.05) increase in total white blood cell (WBC) and heterophil counts throughout the days of the experiment. The increase in the total white blood cell counts was observed three days pi in the cockerels. There was reduction of lymphocytes in unvaccinated but infected cockerels and turkeys three days pi. The erythrocyte and leukocyte values in unvaccinated but infected cockerels and turkeys were similar. The values were lower than those recorded in vaccinated and infected birds. Thus, vaccination against the disease can ameliorate the adverse effect of the virus on haematological parameters. Vaccination of the birds against velogenic Newcastle disease virus reduced morbidity and mortality.

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“…These cause direct losses also interact with bacteria such as Mycoplasma gallisepticum (Mg) and Mycoplasma synoviae (Ms), which exacerbate respiratory diseases. Serology shows ND is widespread throughout Ethiopia (Tadesse et al 2005; Zeleke et al 2005; Regasa et al 2007; Mazengia et al 2010; Chaka et al 2012), and virulent and lentogenic strains have been isolated (Chaka et al 2013; Fentie et al 2014). Vaccines, produced locally, are used in commercial farms, multiplication centres (Dessie and Jobre 2004) and more recently in field trials for village birds (Nega et al 2012).…”

Section: Introductionmentioning

confidence: 99%

Detection of infectious bronchitis virus 793B, avian metapneumovirus, Mycoplasma gallisepticum and Mycoplasma synoviae in poultry in Ethiopia

Hutton

Bettridge

Christley

et al. 2016

Trop Anim Health Prod

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A survey was conducted into respiratory infectious diseases of poultry on a chicken breeder farm run by the Ethiopian Institute of Agricultural Research (EIAR), located in Debre Zeit, Ethiopia. Oropharyngeal swabs were collected from 117 randomly selected birds, and blood was taken from a subset of 73 of these birds. A combination of serological and molecular methods was used for detection of pathogens. For the first time in Ethiopia, we report the detection of variant infectious bronchitis virus (793B genotype), avian metapneumovirus subtype B and Mycoplasma synoviae in poultry. Mycoplasma gallisepticum was also found to be present; however, infectious laryngotracheitis virus was not detected by PCR. Newcastle disease virus (NDV) was not detected by PCR, but variable levels of anti-NDV HI antibody titres shows possible exposure to virulent strains or poor vaccine take, or both. For the burgeoning-intensive industry in Ethiopia, this study highlights several circulating infectious respiratory pathogens that can impact on poultry welfare and productivity.

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Serological and molecular investigation of Newcastle disease in household chicken flocks and associated markets in Eastern Shewa zone, Ethiopia

Cited by 37 publications

References 42 publications

Serological and Molecular Study of Newcastle Disease Virus in Village Chickens in Selected Rift-Valley Areas, Ethiopia

Serological and Molecular Study of Newcastle Disease Virus in Village Chickens in Selected Rift-Valley Areas, Ethiopia

Comparative Haematology of Chickens and Turkeys Experimentally Infected with a Velogenic Newcastle Disease Virus

Detection of infectious bronchitis virus 793B, avian metapneumovirus, Mycoplasma gallisepticum and Mycoplasma synoviae in poultry in Ethiopia

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