Serological and Molecular Investigation of Cutaneous Leishmaniasis in Healthy Individuals from an American Cutaneous Leishmaniasis-Endemic Region

Ribas-Silva, Rejane Cristina; Navasconi, Taísa Rocha; Braga, Laís de Souza; Leatte, Elen Paula; Skraba, Cissiara Maneti; Conter, Carolina Cella; Borelli, Sueli Donizete; Silveira, Thaís Gomes Verzignassi

doi:10.3844/ajidsp.2015.20.25

Cited by 1 publication

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“…Blood samples were collected from patients diagnosed with clinical CL caused by L. braziliensis. This is the main parasite that causes the cutaneous disease in this state of Brazil according to Szargiki et al [ 3 ] and Ribas-Silva et al [ 26 ]. Volunteers came from the Federal University of Paraná's Hospital or several regional departments of health in the same state.…”

Section: Methodsmentioning

confidence: 99%

Synthetic Peptides as Potential Antigens for Cutaneous Leishmaniosis Diagnosis

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Alban

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et al. 2017

Journal of Immunology Research

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This work's goal was to research new candidate antigens for cutaneous leishmaniosis (CL). In order to reach the goal, we used random peptide phage display libraries screened using antibodies from Leishmania braziliensis patients. After selection, three peptides (P1, P2, and P3) were synthesized using Fmoc chemistry. The peptides individually or a mixture of them (MIX) was subsequently emulsified in complete and incomplete Freund's adjuvant and injected subcutaneously in golden hamsters. Sera from the hamsters administered with P1 presented antibodies that recognized proteins between 76 and 150 kDa from L. braziliensis. Sera from hamsters which had peptides P2 and P3, as well as the MIX, administered presented antibodies that recognized proteins between 52 and 76 kDa of L. braziliensis. The research on the similarity of the peptides' sequences in protein databases showed that they match a 63 kDa glycoprotein. The three peptides and the MIX were recognized by the sera from CL patients by immunoassay approach (ELISA). The peptides' MIX showed the best performance (79% sensitivity) followed by the P1 (72% sensitivity), and the AS presented 91% sensitivity. These results show a new route for discovering molecules for diagnosis or for immunoprotection against leishmaniosis.

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Section: Methodsmentioning

confidence: 99%