Serological and molecular screening of umbilical cord blood for <i>Toxoplasma gondii</i> infection

Botein, Eman F; Darwish, Ahmad; El-Tantawy, Nora; Elbaz, Rizk; Eid, Mohamed I; Shaltot, Ali M

doi:10.1111/tid.13117

Cited by 11 publications

(11 citation statements)

References 69 publications

(76 reference statements)

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“…Molecular findings of T. gondii DNA among pregnant women were also found in 24% in the first PCR and nested PCR (10%) of T. gondii targeting at B1 gene, which are varied from the previous molecular detection of T. gondii DNA in line with 10.5% -28.6% (24,30) . However, these results were lower than the former reports of 50% in Libya (20) ; 100% in placental tissue from Wasit province (31) ; 41.7% (PCR), and 86.7% (nested PCR) in Iran (32) .…”

Section: The Agreement Results Among Lat and Pcr Methods Of T Gondii ...mentioning

confidence: 83%

Serological and Molecular Detection of Toxoplasma Gondii among Pregnant Women Giving Birth at the University Of Gondar Specialized Hospital in Gondar Town, Northwest Ethiopia

Yirsa¹,

Indracanti²,

Bandike³

et al. 2022

IJST

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Objective: Congenital toxoplasmosis can be presented when immunosuppressed women are reactivating of this infection acquired before pregnancy in addition to women recently exposed to this disease during pregnancy, which causes of fetal death, neonatal death or congenital defects. Women mainly get this infection by ingested improperly cooked or raw meat consisting of viable tissue cysts or by contaminated foods and water within sporulated oocysts or through the placenta. Thus, this study aimed to detect Toxoplasma gondii in pregnant women using serologically and molecularly at study area. Methods: A cross-sectional study was conducted from September 2019 to October 2020 by collecting a total of 200 umbilical cord blood and matching discharged placental tissue samples from systematic randomly selected pregnant women giving birth at University of Gondar specialized hospital. Latex agglutination test and nested polymerase chain reaction were used for the detections of this infection. Findings: The overall serological, first polymerase chain reaction and nested polymerase chain reaction findings of this study were 46%, 24% and 10%, respectively. The univariant analysis indicated that seropositivity of Toxoplasma gondii antibodies were also significantly influenced by eating raw meat, eating raw vegetables, drinking raw milk, presence of domestic cat contacts and source of water (P ≤ 0.05). In the case of multivariate analysis drinking raw milk and source of water were also significantly associated to the seropositivity of Toxoplasma gondii. Moreover, there were also fair concordant between latex agglutination within first polymerase reaction (Kappa: 0.290

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Section: The Agreement Results Among Lat and Pcr Methods Of T Gondii ...mentioning

confidence: 83%

Serological and Molecular Detection of Toxoplasma Gondii among Pregnant Women Giving Birth at the University Of Gondar Specialized Hospital in Gondar Town, Northwest Ethiopia

Yirsa¹,

Indracanti²,

Bandike³

et al. 2022

IJST

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“…Serum IgM and IgG anti-T. gondii antibodies have been investigated in blood donors aiming to determine the prevalence of infection in different countries as reviewed by Foroutan-Rad and colleagues (Foroutan-Rad et al, 2016;Foroutan et al, 2018) as well as to estimate the risk of transfusional transmission of this parasite (Siransy et al, 2016;Ferreira et al, 2017;Botein et al, 2019;El-Tantawy et al, 2019). However, correlations between serum IgG anti-T. gondii antibody concentrations and the parasitemia determined by molecular methods have not been explored in healthy blood donors.…”

Section: Discussionmentioning

confidence: 99%

“…Molecular methods, such as conventional PCR, Nested PCR, and real-time PCR have been used either in isolation or in association, to detect T. gondii parasitemia in acute and chronically infected individuals since they show high sensitivity (Brenier-Pinchart et al, 2015;Dard et al, 2016;Camilo et al, 2017;Roux et al, 2018;Botein et al, 2019;Greigert et al, 2019). In this study, we used the Nested PCR to target the B1 gene which is one of the most used tests in the literature for detecting T. gondii parasitemia (Okay et al, 2009;Mattos et al, 2011;Teixeira et al, 2013;Roux et al, 2018).…”

Section: Discussionmentioning

confidence: 99%

Serum IgG Anti-Toxoplasma gondii Antibody Concentrations Do Not Correlate Nested PCR Results in Blood Donors

Nakashima

Pardo

Miola

et al. 2020

Front. Cell. Infect. Microbiol.

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Background: Toxoplasma gondii infects millions of individuals worldwide. This protozoan is food and water-borne transmitted but blood transfusion and organ transplantation constitute alternative forms for transmission. However, the influence of IgG anti-T. gondii antibodies in molecular analysis carried out in peripheral blood still remain unclear. This study aimed to investigate the serum IgG anti-T. gondii antibody concentrations correlate Nested PCR results in blood donors. Methods: 750 blood donors were enrolled. IgM and IgG anti-T. gondii antibodies were assessed by ELISA (DiaSorin, Italy). Nested PCR was performed with primers JW62/JW63 (288 bp) and B22/B23 (115 bp) of the T. gondii B1 gene. The mean values of IgG concentration were compared for PCR positive and PCR Negative blood donors using the t-test or Mann-Whitney according to the normal distribution (p-value ≤ 0.05). Results: 361 (48.1%) blood donors presented positive serology as follow: IgM + /IgG − : 5 (0.6%); IgM + /IgG + : 21 (2.8%); IgM − /IgG + : 335 (44.7%) and 389 (51.9%), negative serology. From 353 blood donors with positive serology tested, the Nested PCR was positive in 38 (10.8%) and negative in 315 (89.2%). There were no differences statistically significant between the mean values of serum IgG anti-T. gondii antibody concentrations and the Nested PCR results. Conclusions: In conclusion, our data show that variations in the serum IgG anti-T. gondii antibody concentrations do not correlate T. gondii parasitemia detected by Nested PCR in chronically infected healthy blood donors.

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“…This technique was developed to increase sensitivity and specificity (Jones et al, 2000;Fallahi et al, 2014a) (Table 5). The B1 gene is primarily used for the identification of T. gondii with this type of PCR (Botein et al, 2019;Nakashima et al, 2020;Ngui et al, 2020). However, the Me49, GRA6, and Rep529 genes have also been used as targets (Mousavi et al, 2016;Bahadori et al, 2019;Shahbazi et al, 2019).…”

Section: Polymerase Chain Reaction (Pcr)mentioning

confidence: 99%

Review on diagnosis and molecular characterization of Toxoplasma gondii in humans and animals

A.H.M.M.¹

2021

Trop Biomed

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Toxoplasma gondii is an obligate intracellular protozoon which causes toxoplasmosis, an important zoonotic disease that is endemic worldwide. Common sources of T. gondii infection in humans are food or water contaminated with oocysts and raw or undercooked meat with cysts. In animals, common sources of infection include feed, water, or litter contaminated with oocysts. The diagnosis and molecular characterization of T. gondii infection in humans and animals is crucial due to public and veterinary health importance. Various traditional and serological methods have been used in clinical practice for toxoplasmosis diagnosis, but interpreting the results remains a challenge. Several molecular techniques have also been used for the detection and genetic characterization of T. gondii, but primarily in research settings. In this paper, we review the techniques that are currently used for the diagnosis and genetic characterization of T. gondii in humans and animals, along with their advantages and disadvantages. The techniques reviewed have laid the groundwork for the future development of more effective and precise detection and characterization of T. gondii. These advances will contribute to a better understanding of epidemiology, prevention and control of toxoplasmosis. Thus, this review would be of particular interest to clinical physicians, veterinarians and researchers.

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Serological and molecular screening of umbilical cord blood for Toxoplasma gondii infection

Cited by 11 publications

References 69 publications

Serological and Molecular Detection of Toxoplasma Gondii among Pregnant Women Giving Birth at the University Of Gondar Specialized Hospital in Gondar Town, Northwest Ethiopia

Serological and Molecular Detection of Toxoplasma Gondii among Pregnant Women Giving Birth at the University Of Gondar Specialized Hospital in Gondar Town, Northwest Ethiopia

Serum IgG Anti-Toxoplasma gondii Antibody Concentrations Do Not Correlate Nested PCR Results in Blood Donors

Review on diagnosis and molecular characterization of Toxoplasma gondii in humans and animals

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