Serological comparison of systemic lupus erythematosus with neuropsychiatric lupus using synthetic nucleic acid antigens

Khatri, Sangita; Psaraftis, Nikos; Funaro, Alessia; Arinuma, Yoshiyuki; Fujieda, Yuichiro; Mader, Simone; Jorgensen, C; Astakhova, Kira

doi:10.1016/j.jtauto.2020.100068

Cited by 3 publications

(6 citation statements)

References 33 publications

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“…Estos nuevos antígenos demostraron una sensibilidad analítica hasta 10 veces mayor que el ADN de timo de ternero de control y el kit comercial de anti-ssDNA. Se encontró una asociación significativa entre características clínicas específicas y anticuerpos antinucleares, y se confirmó una asociación entre un anticuerpo anti-dsDNA particular, D5, y el índice de actividad de la enfermedad del LES (SLEDAI) (11).…”

Section: Anticuerpos Anti-adn De Doble Cadenaunclassified

Lupus eritematoso Sistémico: Manifestaciones Neurológicas y Neuropsiquiátricas

Stacey Bustamante,

Lituma Lituma,

Rodríguez Álvarez

et al. 2024

Ciencia Latina

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El lupus eritematoso sistémico (LES) se manifiesta con una amplia gama de síntomas, patrones impredecibles y manifestaciones ocasionales específicas de órganos, lo que dificulta su diagnóstico. A pesar de la falta de pruebas diagnósticas específicas, la evaluación clínica sigue siendo crucial, aunque en algunos casos no se obtienen resultados serológicos positivos. Los últimos criterios diagnósticos, como los criterios EULAR/ACR 2019, facilitan una clasificación más temprana y precisa. Existen varios subtipos de la enfermedad, con algunos pacientes que presentan inicialmente síntomas leves que pueden progresar a una enfermedad grave con el tiempo. Uno de los subtipos del LES son los síndromes neuropsiquiátricos cuya nomenclatura en inglés es NPSLE, se trata sobre una manifestación menos comprendida y posiblemente más prevalente del lupus. Su patogénesis implica varios factores, incluyendo citocinas inflamatorias, autoanticuerpos y complejos inmunes, resultando en daño neuronal. El manejo de LESNP es variado, incluyendo antiinflamatorios, anticoagulantes e inmunosupresores. El tratamiento tiene como objetivo garantizar la supervivencia del paciente, prevenir brotes y daño orgánico, y mejorar la calidad de vida. La terapia inmunosupresora de alta intensidad se utiliza a menudo inicialmente para casos que amenazan la vida u órganos, seguida de una terapia de mantenimiento. En esta investigación detallaremos los principales síntomas neurológicos y neuropsiquiátricos asociados al LES.

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Section: Anticuerpos Anti-adn De Doble Cadenaunclassified

Lupus eritematoso Sistémico: Manifestaciones Neurológicas y Neuropsiquiátricas

Stacey Bustamante,

Lituma Lituma,

Rodríguez Álvarez

et al. 2024

Ciencia Latina

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“…Conventionally for diagnosis and following- up the disease activity, antids-DNA antibodies can be detected by immune assay like immunoassay like enzyme-linked immunosorbent assay (ELISA) [15]. As a comparison of patients with LS, Khatri et al [13 ▪ ] also measured the diversity of antids-DNA antibodies against DNA and LNA/DNA antigens from ds-DNA. In patients with active SLE, higher binding levels for all antigens were found with a lower sensitivity to a particular sequence.…”

Section: Autoantibodies Against Doubles-tranded Dna In Systemic Lupus...mentioning

confidence: 99%

“…Nevertheless, the influence of LNA was similar for LS and SLE. Therefore, they also conducted the study by using DNA and LNA/DNA antigens to test whether there was utility in SLE, especially focusing on discriminating NPSLE from SLE without NPSLE [13 ▪ ].…”

Section: Autoantibodies Against Doubles-tranded Dna In Systemic Lupus...mentioning

confidence: 99%

“…Khatri et al [13 ▪ ] conducted the study to reveal differences of the reactivity in serum antinucleic antibodies against synthetic antigens between NPSLE and SLE using ELISA and affinity chromatography. The major goal of their study was to develop a sensitive, specific and reproducible method to detect autoantibodies in patients with NPSLE.…”

Section: Synthetic Nucleic Acid Antigens In Systemic Lupus Erythemato...mentioning

confidence: 99%

“…To understand NPSLE is difficult because of the absence of a single gold standard to diagnose it. Recently, experiments have focused on the association of autoantibodies against their novel antigens coming from doublestranded DNA (ds-DNA) and NPSLE, especially by the novel methods of using synthetic nucleic acids [13 ▪ ]. First, in this essay, I will review the possibility of synthetic nucleic acids regarding one of the specific epitopes of DNA, RNA or locked nucleic acid (LNA) for detecting SLE and discriminating NPSLE from SLE without NPSLE as a diagnostic marker.…”

Section: Introductionmentioning

confidence: 99%

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The expanding role of synthetic nucleic acids for diagnosis and treatment

Arinuma¹

2022

Current Opinion in Neurology

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Purpose of reviewThe presence of autoantibodies is a characteristic and diagnostic index of systemic lupus erythematosus (SLE). Antidouble-stranded DNA (antids-DNA) antibodies are the most frequent autoantibodies found in SLE related to the diagnosis and disease activity of SLE, and are measured by established methods like ELISA as a polyclonal autoantibody. However, there is no reliable data on the relationship between the respective reactivity of these polyclonal antids-DNA antibodies against different epitopes generated from the original antigen and the disease phenotype. Of the complications in SLE, neuropsychiatric SLE (NPSLE) is a troublesome and frequent phenotype of the disease but no specific diagnostic autoantibodies in serum have been found. First in this review, the possibility of antids-DNA antibodies for identifying primary NPSLE in patients with SLE based on the reactivity of different synthetic nucleic acids is described as a diagnostic marker. The purpose of this review is to examine diagnostic and therapeutic opportunities to modulate autoimmune in the central nervous system (CNS) developing the CNS inflammatory disorders. Recent findingsKhatri et al. investigated antids-DNA antibodies in order to develop a reliable method based on the application of synthetic nucleic acids and protein-based antigen arrays to characterize autoreactive antibodies specially for NPSLE. They found autoantibodies in three particular synthetic double stranded antigens and the antinuclear antibody patterns in ordinary lupus and NPSLE. These discoveries are leading to precision medicine in the CNS inflammatory disorders.

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The Discrepancy of ANA and Compartment Bead Patterns Suggestive of a Neuropsychiatry Systemic Lupus Erythematosus (NPSLE)

Fitriah,

Rahmawati,

Wulanda

et al. 2023

Case Reports in Psychiatry

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Neuropsychiatric systemic lupus erythematosus (NPSLE) exhibits neurological and psychiatric manifestations in systemic lupus erythematosus (SLE) patients, which NPSLE diagnosis can be challenging for rheumatologists. An Indonesian female, 44 years old, complained of two times seizures with 10-min duration, which during seizures were stiff, eyes rolled up, foaming at the mouth, wet the bed, and fainting afterward. The patient also has a history of SLE and received cyclophosphamide therapy 5 years ago. Her clinical condition showed facial and lingual palsy, with central type on the right. Antinuclear antibody indirect immunofluorescence (ANA IF) positive using cytobead ANA with a homogenous pattern and cytoplasmic speckled titer 1/80. Confirmation beads showed positive of dsDNA only. ANA profile showed positive antinucleosome, antihistone, and AMA-M2, and also increased anticardiolipin antibody that supports the diagnosis of NPSLE. The difference in the pattern of ANA IF with confirmation beads suggests the presence of other autoantibodies in NPSLE.

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Serological comparison of systemic lupus erythematosus with neuropsychiatric lupus using synthetic nucleic acid antigens

Cited by 3 publications

References 33 publications

Lupus eritematoso Sistémico: Manifestaciones Neurológicas y Neuropsiquiátricas

Lupus eritematoso Sistémico: Manifestaciones Neurológicas y Neuropsiquiátricas

The expanding role of synthetic nucleic acids for diagnosis and treatment

The Discrepancy of ANA and Compartment Bead Patterns Suggestive of a Neuropsychiatry Systemic Lupus Erythematosus (NPSLE)

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