Serological crossreactivity between Brucella abortus and Yersinia enterocolitica 0:9 III. Specificity of the in vitro antigen-specific gamma interferon test for bovine brucellosis diagnosis in experimentally Yersinia enterocolitica 0:9-infected cattle

Kittelberger, Reinhold; Reichel, Michael P.; Joyce, Maree A.; Staak, C.

doi:10.1016/s0378-1135(97)00110-7

Cited by 29 publications

(10 citation statements)

References 21 publications

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“…This could be attributed to the lower specificity of the ELISA. Previous studies have reported cross-reactivity between Yersinia, Brucella (2,(17)(18)(19), R. rickettsii (2,23), and TSI (1,2,13,24). Cross-reactivity between F. tularensis and Brucella spp.…”

Section: Discussionmentioning

confidence: 95%

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Evaluation of a Western Blot Method for the Detection of Yersinia Antibodies: Evidence of Serological Cross-Reactivity between Yersinia Outer Membrane Proteins and Borrelia burgdorferi

Rawlins

Gerstner

Hill

et al. 2005

Clin Vaccine Immunol

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Yersinia enterocolitica and Yersinia pseudotuberculosis have been identified as causative organisms of reactive arthritis in humans. We evaluated a Western blot assay which uses Yersinia outer membrane proteins as antigens for the detection of Yersinia antibodies as a replacement for the complement fixation (CF) assay. Clinical agreement, sensitivity, and specificity were determined by testing 19 positive and 21 negative serum samples by the CF assay, Western blot assay, and enzyme-linked immunosorbent assay (ELISA). The CF assay and ELISA were compared to the Western blot assay, which was the reference method used in this study. Sera with antibodies that could potentially cross-react with Yersinia were also tested by the Western blot assay. The agreement, sensitivity, and specificity of the CF method were 61%, 26%, and 95%, respectively; and those for the ELISA were 89%, 95%, and 82%, respectively. The prevalences of Yersinia antibodies in 50 healthy donors were 6% for immunoglobulin G (IgG), 2% for IgA, and 2% for IgM. Sera positive for Bartonella henselae, Brucella, Chlamydia pneumoniae, and Rickettsia rickettsii antibodies showed cross-reactivity by the Western blot assay. The highest cross-reactivity was observed with Borrelia burgdorferi; 5 of 11 (45%) specimens were cross-reactive by the IgM-specific assay. Overall, the Western blot assay performs acceptably and is more sensitive than the CF assay, warranting replacement of the CF assay in the laboratory. Due to the evidence of cross-reactivity, particularly with B. burgdorferi, which can cause an oligoarthritis similar to reactive arthritis, the diagnosis of reactive arthritis should be based on clinical findings and complete serologic analysis of the potential causative infectious pathogens.

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Section: Discussionmentioning

confidence: 95%

“…The cross-reactivity of Yersinia with other bacterial species, such as Borrellia burgdorferi (3,25), Rickettsia rickettsii (2,23), and Brucella spp. (2,(17)(18)(19), has been reported. Additionally, crossreactivity between Yersinia and thyroid-stimulating immunoglobulin (TSI) in patients with Graves ' disease has been shown (1,2,13,24).…”

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confidence: 99%

Evaluation of a Western Blot Method for the Detection of Yersinia Antibodies: Evidence of Serological Cross-Reactivity between Yersinia Outer Membrane Proteins and Borrelia burgdorferi

Rawlins

Gerstner

Hill

et al. 2005

Clin Vaccine Immunol

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“…, serum dilution, antigen concentration, cut-off, etc) as recommended by manufacturers in domestic livestock [14,17,47], and therefore without adequate validation for the corresponding wild species tested. Moreover, the problem of the false-positive serological reactions induced by gram-negative bacteria sharing common epitopes with Brucella [48,49], is also an important issue to properly assess brucellosis prevalence. Hence, recent studies suggest the need for better diagnostic tools to obtain reliable results in serological studies on brucellosis in wildlife [17].…”

Section: Discussionmentioning

confidence: 99%

Spatial distribution and risk factors of Brucellosis in Iberian wild ungulates

et al. 2010

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BackgroundThe role of wildlife as a brucellosis reservoir for humans and domestic livestock remains to be properly established. The aim of this work was to determine the aetiology, apparent prevalence, spatial distribution and risk factors for brucellosis transmission in several Iberian wild ungulates.MethodsA multi-species indirect immunosorbent assay (iELISA) using Brucella S-LPS antigen was developed. In several regions having brucellosis in livestock, individual serum samples were taken between 1999 and 2009 from 2,579 wild bovids, 6,448 wild cervids and4,454 Eurasian wild boar (Sus scrofa), and tested to assess brucellosis apparent prevalence. Strains isolated from wild boar were characterized to identify the presence of markers shared with the strains isolated from domestic pigs.ResultsMean apparent prevalence below 0.5% was identified in chamois (Rupicapra pyrenaica), Iberian wild goat (Capra pyrenaica), and red deer (Cervus elaphus). Roe deer (Capreolus capreolus), fallow deer (Dama dama), mouflon (Ovis aries) and Barbary sheep (Ammotragus lervia) tested were seronegative. Only one red deer and one Iberian wild goat resulted positive in culture, isolating B. abortus biovar 1 and B. melitensis biovar 1, respectively. Apparent prevalence in wild boar ranged from 25% to 46% in the different regions studied, with the highest figures detected in South-Central Spain. The probability of wild boar being positive in the iELISA was also affected by age, age-by-sex interaction, sampling month, and the density of outdoor domestic pigs. A total of 104 bacterial isolates were obtained from wild boar, being all identified as B. suis biovar 2. DNA polymorphisms were similar to those found in domestic pigs.ConclusionsIn conclusion, brucellosis in wild boar is widespread in the Iberian Peninsula, thus representing an important threat for domestic pigs. By contrast, wild ruminants were not identified as a significant brucellosis reservoir for livestock.

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“…False positive results for B. abortus antibody can occur in cattle vaccinated against B. abortus <4 months prior to testing, or in milk containing colostrum or from cows with mastitis. Infection with other pathogens, such as Yersinia enterocolitica, can also cause nonspecific positive reactions (Kittelberger et al, 1997). Comparable results are obtainable with the fluorescence polarisation assay (Nielsen and Gall, 2001), and this newer test can be used in the field.…”

Section: Milkmentioning

confidence: 99%

Moving past serology: Diagnostic options without serum

Reichel

Lanyon

Hill³

2016

The Veterinary Journal

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Detecting antibodies formed in serum in response to infection is the traditional function of serology. Diagnostic modalities have included complement fixation tests, agar gel immune-diffusion, radioimmunoassay, ELISA and immunofluorescence. More recent technology now allows for the direct detection of pathogens by PCR. This review details the options for diagnostic testing using specimen types other than serum, identifying the advantages and disadvantages of these options and providing evidence for more widespread use of these techniques and specimen types.

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Serological crossreactivity between Brucella abortus and Yersinia enterocolitica 0:9 III. Specificity of the in vitro antigen-specific gamma interferon test for bovine brucellosis diagnosis in experimentally Yersinia enterocolitica 0:9-infected cattle

Cited by 29 publications

References 21 publications

Evaluation of a Western Blot Method for the Detection of Yersinia Antibodies: Evidence of Serological Cross-Reactivity between Yersinia Outer Membrane Proteins and Borrelia burgdorferi

Evaluation of a Western Blot Method for the Detection of Yersinia Antibodies: Evidence of Serological Cross-Reactivity between Yersinia Outer Membrane Proteins and Borrelia burgdorferi

Spatial distribution and risk factors of Brucellosis in Iberian wild ungulates

Moving past serology: Diagnostic options without serum

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