Serological crossreactivity between Brucella abortus and Yersinia enterocolitica 0:9 I Immunoblot analysis of the antibody response to Brucella protein antigens in bovine brucellosis

Kittelberger, Reinhold; Hilbink, F.; Hansen, Michael A.; Penrose, M.; Lisle, Geoffrey W. de; Letesson, Jean‐Jacques; Garin‐Bastuji, Bruno; Searson, J. E.; Fossati, Carlos A.; Cloeckaert, Axel

doi:10.1016/0378-1135(95)00122-0

Cited by 33 publications

(33 citation statements)

References 27 publications

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“…These protein bands disappeared completely on the Coomassie-stained gel after enzymatic digestion, but the silver-stained gels still showed strong staining for digested antigen preparations. The staining patterns were strong and diffuse over a wide MW range (20-200 kDa), which is typical for smooth lipopolysaccharides (SLPSs) (Tomás et al, 1986;Kittelberger et al, 1995). After running a commercial rabbitanti Klebsiella serum and a number of NZSL samples in the Klebs-WB, it became obvious that SLPS was immunodominant and that the three antigen preparations were immunologically identical.…”

Section: Optimization Of Wb Methods (Klebs-wb and Igg-wb)mentioning

confidence: 99%

“…The supernatants were used as antigens, and they were designated AG1 (K. pneumoniae 1), AG2 (K. pneumoniae 2), and AG3 (K. oxytoca). To further characterize antigens (lipopolysaccharides or proteins) and to optimize the WB technique, aliquots of the three crude antigens were incubated with proteinase K (pK, at 60 mAU/ml) at 56 C for 1 hr, and then they were analyzed by SDS-PAGE using Coomassie and silver staining methods as described by Kittelberger et al (1994).…”

Section: Preparation Of Antigensmentioning

confidence: 99%

“…Gels were run for 40 min at 200 V, and then either used for WB or for gel staining (Kittelberger et al, 1994). Gels with 10 or 15 wells were used for the analysis of antigens for the WB optimization; preparative gels were used for the screening of serum samples for anti-Klebsiella antibodies (Klebs-WB).…”

Section: Sds-page and Wbmentioning

confidence: 99%

“…(Aschfalk et al, 2002;Nielsen et al, 2005), the Western blot (WB) technique may represent an alternative to ELISA. The WB has shown comparable sensitivity and specificity for detection of antibodies against a variety of bacterial infections (Kittelberger et al, 1995). Unlike ELISA, the WB is not as dependent upon a negative serum panel for the calculation of a specific cut-off value.…”

Section: Introductionmentioning

confidence: 99%

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Humoral Immune Response to Klebsiella Spp. In New Zealand Sea Lions (Phocarctos Hookeri) and the Passive Transfer of Immunity to Pups

Castinel

Kittelberger²,

Pomroy

et al. 2008

Journal of Wildlife Diseases

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ABSTRACT:During the 2001-02 and 2002-03 breeding seasons, epizootics of Klebsiella pneumoniae resulted in a dramatic increase of pup mortality in New Zealand sea lions (Phocarctos hookeri; NZSLs) on Enderby Island (Auckland Islands). To estimate the prevalence of infection in the NZSL population, a serologic test was developed using a Western blot and a polysaccharide antigen derived from a K. pneumoniae isolate from a NZSL pup. All archived serum samples collected between 1997 and 1998 and 2004 and 2005 at Sandy Bay Beach rookery, Enderby Island, were tested (314 pups and 302 adult females). Anti-Klebsiella antibodies were detected throughout this period, but overall, only 16% of NZSL pups between birth and 5 mo of age were seropositive compared with 95.7% of adults. There was no apparent change in antibody prevalence as a result of the two epizootics. A method to determine total immunoglobulin G (IgG) levels in sea lion serum also was developed to investigate passive immunoglobulin transfer to neonates and development of an acquired immune response. The IgG concentration was significantly lower in pups (median 2.1 mg/ml) than in adult females (median 80 mg/ml). Based on serologic results, it was not possible to determine whether K. pneumoniae was an endemic or a novel pathogen to the NZSL population because the test was not able to discriminate between Klebsiella species. However, this study suggested that the transfer of passive immunity to neonates was very low in the NZSL, especially for anti-Klebsiella antibodies.

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Section: Optimization Of Wb Methods (Klebs-wb and Igg-wb)mentioning

confidence: 99%

Section: Preparation Of Antigensmentioning

confidence: 99%

Section: Sds-page and Wbmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Humoral Immune Response to Klebsiella Spp. In New Zealand Sea Lions (Phocarctos Hookeri) and the Passive Transfer of Immunity to Pups

Castinel

Kittelberger²,

Pomroy

et al. 2008

Journal of Wildlife Diseases

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“…25 Previous studies have noted cross-reactivity of Y09 with B. abortus serologic tests because of common antigenic epitopes of the smooth LPS O chain between these species. 13,19,24 Although marine mammal infection with Y09 has not yet been reported, and a previous study did not detect Y09 antibodies in serum from various marine mammals, 33 the potential for transmission theoretically exists. Knowledge of potential cross-reaction of the marine cELISA with Y09 antibodies will help differentiate between these pathogens.…”

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confidence: 98%

Development, Validation, and Utilization of a Competitive Enzyme-Linked Immunosorbent Assay for the Detection of Antibodies against Brucella Species in Marine Mammals

et al. 2010

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Abstract. A competitive enzyme-linked immunosorbent assay (cELISA) was developed by using a wholecell antigen from a marine Brucella sp. isolated from a harbor seal (Phoca vitulina). The assay was designed to screen sera from multiple marine mammal species for the presence of antibodies against marine-origin Brucella. Based on comparisons with culture-confirmed cases, specificity and sensitivity for cetacean samples tested were 73% and 100%, respectively. For pinniped samples, specificity and sensitivity values were 77% and 67%, respectively. Hawaiian monk seal (Monachus schauinslandi; n 5 28) and bottlenose dolphin (Tursiops truncatus; n 5 48) serum samples were tested, and the results were compared with several other assays designed to detect Brucella abortus antibodies. The comparison testing revealed the marine-origin cELISA to be more sensitive than the B. abortus tests by the detection of additional positive serum samples. The newly developed cELISA is an effective serologic method for detection of the presence of antibodies against marine-origin Brucella sp. in marine mammals.

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Y. enterocolitica and Y. pseudotuberculosis

Carniel¹,

Autenrieth²,

Cornelis³

et al. 2006

The Prokaryotes

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Serological crossreactivity between Brucella abortus and Yersinia enterocolitica 0:9 I Immunoblot analysis of the antibody response to Brucella protein antigens in bovine brucellosis

Cited by 33 publications

References 27 publications

Humoral Immune Response to Klebsiella Spp. In New Zealand Sea Lions (Phocarctos Hookeri) and the Passive Transfer of Immunity to Pups

Humoral Immune Response to Klebsiella Spp. In New Zealand Sea Lions (Phocarctos Hookeri) and the Passive Transfer of Immunity to Pups

Development, Validation, and Utilization of a Competitive Enzyme-Linked Immunosorbent Assay for the Detection of Antibodies against Brucella Species in Marine Mammals

Y. enterocolitica and Y. pseudotuberculosis

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