Serological detection and epidemiology of Neospora caninum and Cryptosporidium parvum antibodies in cattle in southern Egypt

Fereig, Ragab M.; AbouLaila, Mahmoud; Mohamed, Samy G.A.; Mahmoud, Hassan Y.A.H.; Ali, Alsagher O.; Ali, Asmaa F.; Hilali, M.; Anis, Anis; Mohamed, Adel Elsayed Ahmed; Nishikawa, Yoshifumi

doi:10.1016/j.actatropica.2016.06.032

Cited by 32 publications

(28 citation statements)

References 36 publications

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“…In comparison, it was (53%) among positive samples Table (2), these results were agreed with that recorded by (Dubey et al, 1998) where the prevalence of N. caninum antibodies were 68% and 20.43% in buffalo and cattle, respectively in the delta region in Egypt. Our results are higher than that reported by (Fereig et al, 2016), where they revealed that 18.9% of examined cattle were positive for N. caninum. Also, NC prevalence was high compared to other studies that had reported prevalence ranging from 1.9%-39.7% (Konnai et al, 2008;Asmare et al, 2013).…”

Section: Discussioncontrasting

confidence: 94%

Investigation of The Association Between Bovine Viral Diarrhea Virus and Neospora caninum as a Cause of Abortion in Cattle

El-Mohamady

A.M.

Y.G.M.

2021

Journal of Applied Veterinary Sciences

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This study aimed to investigate the complementary association between Bovine Viral Diarrhea Virus (BVDV) and Neospora caninum (NC) in the induction of abortion in cattle. The study was carried out on 116 serum samples collected from 116 aborted cows in different Egyptian localities. All blood samples were immediately transported to the diagnostic laboratory. Serum was harvested after centrifugation at 1500×g for 10 min. All sera were divided equally into two microtubes and stored at -20 °C until laboratory testing. Double antibody sandwich immunoenzymatic assay (DS.ELISA) is used for bovine viral diarrhea virus (BVDV) p80/p125 antigen detection and Anti-Neospora caninum (NC) antibodies were detected using an indirect ELISA. Our results cleared that the incidences of BVDV and NC among examined cow's serum samples were 66 (56.9 %), where the incidences of BVDV and NC were 31 (26.72 %) and 35 (30.17 %), respectively. There were 18 (27.3 %) samples that showed mixed infection BVDV and NC incidences among examined cattle serum samples. The study concluded that the prevalence of BVDV and NC are high and widely spread in cattle farms in Egypt. There is a pronounced association between BVDV and NC and concurrent infection in cattle farms in Egypt. So we should coordinate surveillance and control programs on abortifacient pathogens (BVDV and NC) should be developed and implemented, which would reduce the losses associated with these diseases in cattle farms.

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Section: Discussioncontrasting

confidence: 94%

Investigation of The Association Between Bovine Viral Diarrhea Virus and Neospora caninum as a Cause of Abortion in Cattle

El-Mohamady

A.M.

Y.G.M.

2021

Journal of Applied Veterinary Sciences

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“…In animals, from 1999 to 2016 the prevalence rate of Cryptosporidium infection ranged between 2% and 69% among different species including cattle, buffalo calves, camels, sheep, goats, lambs, dogs, wild rats and quails. The highest prevalence rates were reported in governorates that located close to the River Nile [ 169 , 170 , 171 , 172 , 173 , 174 , 175 , 176 , 177 , 178 , 179 , 180 , 181 ]. In humans, between 1989 and 2016 Cryptosporidium infection has been reported in almost all Egyptian governorates with prevalence rates ranged between 3% and 50% or up to 91% in immunocompromised patients and diarrheic children [ 169 , 170 , 171 , 181 , 182 , 183 , 184 , 185 , 186 , 187 , 188 , 189 , 190 , 191 , 192 , 193 , 194 , 195 , 196 , 197 ].…”

Section: Parasitic Zoonosismentioning

confidence: 99%

A Comprehensive Review of Common Bacterial, Parasitic and Viral Zoonoses at the Human-Animal Interface in Egypt

2017

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Egypt has a unique geographical location connecting the three old-world continents Africa, Asia and Europe. It is the country with the highest population density in the Middle East, Northern Africa and the Mediterranean basin. This review summarizes the prevalence, reservoirs, sources of human infection and control regimes of common bacterial, parasitic and viral zoonoses in animals and humans in Egypt. There is a gap of knowledge conerning the epidemiology of zoonotic diseases at the human-animal interface in different localities in Egypt. Some zoonotic agents are “exotic” for Egypt (e.g., MERS-CoV and Crimean-Congo hemorrhagic fever virus), others are endemic (e.g., Brucellosis, Schistosomiasis and Avian influenza). Transboundary transmission of emerging pathogens from and to Egypt occurred via different routes, mainly importation/exportation of apparently healthy animals or migratory birds. Control of the infectious agents and multidrug resistant bacteria in the veterinary sector is on the frontline for infection control in humans. The implementation of control programs significantly decreased the prevalence of some zoonoses, such as schistosomiasis and fascioliasis, in some localities within the country. Sustainable awareness, education and training targeting groups at high risk (veterinarians, farmers, abattoir workers, nurses, etc.) are important to lessen the burden of zoonotic diseases among Egyptians. There is an urgent need for collaborative surveillance and intervention plans for the control of these diseases in Egypt.

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“…On the other hand, animal husbandry systems in Egypt, where most farms located near to rural areas of villages sus-tain the favorable environment for N. caninum, due to the presence of dogs with high numbers roaming freely around and enter the cattle farms and the lack of veterinary attention. The scarce researches on N. caninum in Egypt showed that it is endemic at moderate levels; 68%, in buffaloes (Dubey et al, 1998), 3.6% in camels (Hilali et al, 1998), 20.43% in cattle, 1.85% in rabbits and 7.92% in human in Northern Egypt (Ibrahim et al, 2009), 14.75% in chickens in Kafrelsheikh (Ibrahim et al, 2013), and 18.9% in cattle in southern Egypt (Fereig et al, 2016).…”

Section: Introductionmentioning

confidence: 99%

“…Antibodies in the serum of infected animal are very important for diagnosis of N. caninum infection, which could be detected by different serological tests, including: immunoblotting (IB), direct agglutination test (DAT), indirect fluorescent antibody test (IFAT) and enzyme-linked immunosorbent assays (ELISAs) (Söndgen et al, 2001, Moraveji et al, 2012, Moore et al, 2014, Hamidinejat et al, 2015. Serological surveys for capturing sero-prevalence data are much required for control neosporosis (Fereig et al, 2016). Therefore, a sero-survey for N. caninum infection among cows that aborted in dairy herds may highlight the importance of the neglected role of such protozoan for abortion in Egyptian farms and withdraw the attention towards different causes of abortion other than brucellosis.…”

Section: Introductionmentioning

confidence: 99%

Neosporosis: a neglected abortifacient disease in Egypt, seroprevalence and farmers’ knowledge, attitudes and practices

Gaber

Hegazy

Oreiby

et al. 2021

J Hellenic Vet Med Soc

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Neosporosis has emerged as a serious disease of cattle and dogs worldwide, causing abortions, neonatal mortalities and massive economic losses in dairy herds. The current study aimed to investigate the burden of Neospora (N.) caninum infection among cows that aborted and to assess knowledge, attitudes, and practices (KAPs) of farmers toward neosporosis in Kafrelsheikh governorate, Egypt. A total of 92 cows that aborted and 25 heifers born from cows that aborted and seropositive for N. caninum infection from 15 dairy herds in different districts of Kafrelsheikh governorate were examined serologically against N. caninum infection using ELISA. A structured questionnaire was built and distributed to 41 farmers in the study area. The overall seroprevalence of N. caninum infection among the examined cows that aborted was 38.04% (35/92). On the other hand, the prevalence of N. caninum infection among the 25 examined heifers born from seropositive cows that aborted was 28% (7/25). The KAPs analysis showed that farmers lack the required information on N. caninum infection and its consequences on dairy farms. The farmers performed risky practices which are responsible for disease entrance and spread on the farm; buy and keeping animals after abortion as well as their heifers. In addition, risk management practices were widespread in dairy farms such as: free movement of dogs, especially stray ones, in 100% of the farms, dogs were able to access and defecate in cattle feed and drinking water sources and had the chance to eat placentas and abortion materials. The current study points out neosporosis as a neglected cause of abortion among cattle in Kafrelsheikh governorate, as well as there is a lack of knowledge and risky practices by the local farmers. These findings may be extrapolated to nearby areas and countries of the same cattle husbandry practices, and veterinary services in such countries should consider neosporosis in their surveillance and control programs.

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Serological detection and epidemiology of Neospora caninum and Cryptosporidium parvum antibodies in cattle in southern Egypt

Cited by 32 publications

References 36 publications

Investigation of The Association Between Bovine Viral Diarrhea Virus and Neospora caninum as a Cause of Abortion in Cattle

Investigation of The Association Between Bovine Viral Diarrhea Virus and Neospora caninum as a Cause of Abortion in Cattle

A Comprehensive Review of Common Bacterial, Parasitic and Viral Zoonoses at the Human-Animal Interface in Egypt

Neosporosis: a neglected abortifacient disease in Egypt, seroprevalence and farmers’ knowledge, attitudes and practices

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