Serological Detection of Caseous Lymphadenitis in Sheep and Goats Using a Commercial ELISA in Grenada, West Indies

Hariharan, Harry; Tiwari, Keshaw; Kumthekar, Sachin; Thomas, D.; Hegamin-Younger, Cecilia; Edwards, Bryan; Sharma, Rakesh

doi:10.5171/2015.473459

Cited by 4 publications

(3 citation statements)

References 16 publications

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“…Nos testes ELISA têm sido utilizado vários preparados antigênicos como a parede celular bacteriana, que contém muitas proteínas imunologicamente ativas (STING et al, 1998) Comercialmente, estão disponíveis "kits" de ELISA, como o ELITEST CLA, um produto comercial da Hyphen BioMed, na França, que detecta anticorpos IgG específicos para o agente causal da LC, em soros ovinos e caprinos. Este teste foi utilizado por Hariharan et al, (2015), em Granada, em estudo de prevalência da LC em pequenos ruminantes. Habasha e Al-Badrawi (2016) também fizeram uso deste kit para detectar anticorpos IgG anti-PLD, mas em soros de ovinos em Bagdá e arredores.…”

Section: Ensaio De Imunoabsorção Enzimática (Elisa)unclassified

Ciências Rurais em Foco - Volume 10

2023

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Modo de acesso: World Wide Web Inclui bibliografia 1. Agricultura 2. Meio Ambiente 3. Zootecnia 4. Ciências Agrárias I. REDIN, Ezequiel II. Título. CDD-630 Sônia Márcia Soares de Moura -CRB 6/1896 O conteúdo deste livro está licenciado sob a Licença de Atribuição Creative Commons 4.0. Com ela é permitido compartilhar o livro, devendo ser dado o devido crédito, não podendo ser utilizado para fins comerciais e nem ser alterada. O conteúdo dos artigos e seus dados em sua forma, correção e confiabilidade são de responsabilidade exclusiva dos seus respectivos autores www.poisson.com.br

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Section: Ensaio De Imunoabsorção Enzimática (Elisa)unclassified

Ciências Rurais em Foco - Volume 10

2023

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“…CLA is usually confirmed by isolation, culture and biochemical identification of C. pseudotuberculosis to differentiate it from other bacterial organisms that are characterized by formation of pus in goats though this may not be efficient in chronic infection where the external lesions contain little pus and viable organisms (Ribeiro et al, 2013). Serological test like ELISA and PCR are more efficient in diagnosis because they can be used to detect asymptomatic infection in a herd Ribeiro et al, 2013;Hariharan et al, 2015;Nassar et al, 2015) Treatment Treatment with antibiotics have been ineffective (Hariharan et al, 2015) though other methods such as administration of vaccine of the strain isolated from the herd, lancing, flushing and surgical removal of abscess with identification and culling of affected animals have been used (Washburn et al, 2013). Though these methods involve heavy economic implications (Washburn et al, 2013).…”

Section: Diagnosismentioning

confidence: 99%

Nanoparticles Antibiotic Delivery System: An Alternative Treatment Protocol for Caseous Lymphadenitis.

IDRIS,

ARIFAH

2023

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Caseous lymphadenitis is an infectious zoonotic disease of goats with severe economic implications. A major reason why management of the disease is a problem in the small ruminant livestock industry is because Corynebacterium pseudotuberculosis forms biofilm in the host and is resistant to antibiotic drugs resulting in a chronic granulomatous condition. The use of nanoparticles as a means of direct drug delivery to this organism may provide a solution to tackling it. This review focuses on caseous lymphadenitis and the different nanoparticles for antimicrobial drug delivery

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“…It can also infect humans upon occupational exposure [4, 5]. CLA is prevalent in several countries around the world [6–21] and causes significant economic losses due to low carcass quality, a decrease in the production of meat, wool and milk [22, 23], while also causing animal mortality due to suppurative meningoencephalitis [24]. The available methods for CLA diagnosis or treatment are not effective enough and require further research to tackle the threats posed by C. pseudotuberculosis .…”

Section: Introductionmentioning

confidence: 99%

In silico identification of essential proteins in Corynebacterium pseudotuberculosis based on protein-protein interaction networks

et al. 2016

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Background Corynebacterium pseudotuberculosis (Cp) is a gram-positive bacterium that is classified into equi and ovis serovars. The serovar ovis is the etiological agent of caseous lymphadenitis, a chronic infection affecting sheep and goats, causing economic losses due to carcass condemnation and decreased production of meat, wool, and milk. Current diagnosis or treatment protocols are not fully effective and, thus, require further research of Cp pathogenesis.ResultsHere, we mapped known protein-protein interactions (PPI) from various species to nine Cp strains to reconstruct parts of the potential Cp interactome and to identify potentially essential proteins serving as putative drug targets. On average, we predict 16,669 interactions for each of the nine strains (with 15,495 interactions shared among all strains). An in silico sanity check suggests that the potential networks were not formed by spurious interactions but have a strong biological bias. With the inferred Cp networks we identify 181 essential proteins, among which 41 are non-host homologous.ConclusionsThe list of candidate interactions of the Cp strains lay the basis for developing novel hypotheses and designing according wet-lab studies. The non-host homologous essential proteins are attractive targets for therapeutic and diagnostic proposes. They allow for searching of small molecule inhibitors of binding interactions enabling modern drug discovery. Overall, the predicted Cp PPI networks form a valuable and versatile tool for researchers interested in Corynebacterium pseudotuberculosis.Electronic supplementary materialThe online version of this article (doi:10.1186/s12918-016-0346-4) contains supplementary material, which is available to authorized users.

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Serological Detection of Caseous Lymphadenitis in Sheep and Goats Using a Commercial ELISA in Grenada, West Indies

Cited by 4 publications

References 16 publications

Ciências Rurais em Foco - Volume 10

Ciências Rurais em Foco - Volume 10

Nanoparticles Antibiotic Delivery System: An Alternative Treatment Protocol for Caseous Lymphadenitis.

In silico identification of essential proteins in Corynebacterium pseudotuberculosis based on protein-protein interaction networks

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