Serological detection of chicken flocks naturally infected with salmonella enteritidis, using an enzyme‐linked immunosorbent assay based on monoclonal antibodies against the flagellar antigen

Zijderveld, F.G. van; Bemmel, A.M. van Zijderveld‐van; Brouwers, Rebecca; Vries, T. S. de; Landman, W. J. M.; Jong, Wibe A. de

doi:10.1080/01652176.1993.9694391

Cited by 23 publications

(8 citation statements)

References 11 publications

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“…Indirect ELISA was used as the reference method in the present work because of the easy availability of its reagents and of the simplicity and precision of the test procedure. Another ELISA technique, the double antibody sandwich (DAS) blocking ELISA, has been used in other works to test S. enteritidis-infected flocks ( VAN ZIJDERVELD et al, 1993;THORNS et al, 1996). However, the good specificity and better sensitivity of the indirect ELISA in comparison with the blocking ELISA was also demonstrated by a previous study (HOORFAR et al, 1994).…”

Section: Discussionmentioning

confidence: 99%

Detection of Antibodies to S. enteritidis in Broilers by Means of Indirect ELISA and Chemiluminescent Immunoassay (CLIA)

Zamora¹,

Hartung

Hildebrandt

et al. 1999

Journal of Veterinary Medicine, Series B

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This study was conducted to develop a serological detection system for the monitoring of broiler flocks for Salmonella enteritidis infections. A specific S. enteritidis antigen (FG-Antigen) was used to compare the sensitivity and the specificity of the chemiluminescent immunoassay (CLIA) with those of the indirect ELISA. This comparison was performed using a total of 578 sera, which, depending on the microbiological and vaccination history, were categorized into groups. Most of the serum samples which were classified as positive showed higher titers in CLIA than in ELISA. Using the prevalence of positive reactors, significant differences between Groups were additionally demonstrated. The absorbance values of the passively immunized group showed the highest and those of the Salmonella-negative group the lowest correlation-coefficient. Using the mean net absorbance of the prevalence group, the ELISA system exhibited a sensitivity of 100% and a specificity of 96.2%, while CLIA had a sensitivity and a specificity of 85.7% and 96.2%, respectively. ELISA and CLIA can be used in the examination of non vaccinated flocks for S. enteritidis-infections as alternative to the bacteriological culture method. CLIA is distinguished for its fast and convenient procedure as well as for its wider measurement spectrum, while the indirect ELISA is almost as efficient as CLIA and requires less investment.

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Section: Discussionmentioning

confidence: 99%

Detection of Antibodies to S. enteritidis in Broilers by Means of Indirect ELISA and Chemiluminescent Immunoassay (CLIA)

Zamora¹,

Hartung

Hildebrandt

et al. 1999

Journal of Veterinary Medicine, Series B

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“…Bacteriological examination for the detection of flock infections on a practical basis is laborious, time-consuming, and expensive, especially when a statistically reliable number of faecal samples per flock is tested to confirm that a flock found bacteriologically negative for S. enteritidis is truly free of S. enteritidis [11]. Bacteriological examination may also yield false negative results when S. enteritidis is overgrown by other Salmonella serotypes present in the flock [28]. Identification of infected birds is also difficult because S. enteritidis can induce a chronic carrier state, whereby apparently healthy birds excrete the organisms intermittently [31].…”

Section: Introductionmentioning

confidence: 99%

“…This paper evaluates the specificity, sensitivity and discrimination capacity of an indirect ELISA based on the use of four different antigens obtained from a clinical isolate of S. enteritidis, and compares the results to those obtained with the gm-flagellin based ELISA implemented in the Dutch S. enteritidis eradication program [27,28], for detecting infection in naturally infected chickens.…”

Section: Introductionmentioning

confidence: 99%

Enzyme-linked immunosorbent assay with a Salmonella enteritidis antigen for differentiating infected from vaccinated poultry

et al. 2000

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-The specificity and sensitivity of indirect ELISA, based on the use of four different antigenic extracts obtained from a clinical isolate of Salmonella enteritidis, were compared with those obtained with the gm-flagellin based ELISA (IDEXX). A total of 116 serum samples from salmonellae free, naturally infected and vaccinated hens were studied. The results showed that the indirect ELISA, based on lipopolysaccharide (LPS), O-polysaccharide (PS) or membrane sediment (SD) antigens, enable the identification of a greater number of infected birds and discriminated field antibody responses from vaccinal ones better than the commercial IDEXX test. The indirect ELISA that used a O-polysaccharide rich fraction (PS) proved to be the most specific and sensitive test, suggesting that this indirect ELISA could be used to confirm IDEXX results, especially when the differentiation between vaccinated and infected poultry is required. Salmonella / serological diagnosis / poultry / ELISARésumé -ELISA basé sur l'utilisation d'un antigène de Salmonella enteritidis pour la diffé-rentiation entre la volaille infectée et vaccinée. La spécificité et la sensibilité d'une nouvelle méthode ELISA indirecte, basée sur l'utilisation de quatre extraits antigéniques différents obtenus d'un isolat clinique de Salmonella enteritidis, ont été comparées avec celles d'un autre ELISA basé sur l'utilisation de la gm-flagelline (IDEXX). Un total de 116 échantillons de sérum provenant de poules saines (non-infectées avec Salmonella), infectées ou vaccinées, a été analysé par les deux techniques. Les résultats ont clairement démontré que la méthode ELISA indirecte, utilisant les antigènes LPS, une fraction riche en polysaccharide-O (PS) ou le sédiment de membranes SD (LPS-proteins complex), permettait l'identification d'un plus grand nombre d'animaux infectés, et une meilleure différentiation Vet. Res. 31 (2000) [491][492][493][494][495][496][497] 491

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“…A good example is the approach in the poultry industry. Several tests have been developed to determine whether a poultry farm is free of Salmonella enteritidis, the most common being the use of antibodies directed against a specific flagellar antigen, in an EIA (17). This test is very sensitive, and specific for S. enteritidis.…”

Section: Analysis To Enable Decision Makingmentioning

confidence: 99%

Interruption of bacterial cycles in animal production: Related to veterinary public health

Logtestijn

Urlings

Bijker

et al. 1993

Veterinary Quarterly

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Serological detection of chicken flocks naturally infected with salmonella enteritidis, using an enzyme‐linked immunosorbent assay based on monoclonal antibodies against the flagellar antigen

Cited by 23 publications

References 11 publications

Detection of Antibodies to S. enteritidis in Broilers by Means of Indirect ELISA and Chemiluminescent Immunoassay (CLIA)

Detection of Antibodies to S. enteritidis in Broilers by Means of Indirect ELISA and Chemiluminescent Immunoassay (CLIA)

Enzyme-linked immunosorbent assay with a Salmonella enteritidis antigen for differentiating infected from vaccinated poultry

Interruption of bacterial cycles in animal production: Related to veterinary public health

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