Serological detection of exposure to Cryptosporidium parvum in cattle by ELISA and its evaluation in relation to coprological tests

Nydam, D.V.; Lindergard, Gabriella; Guard, C.L.; Schaaf, Stephanie L.; Wade, Susan E.; Mohammed, Hussni O.

doi:10.1007/s00436-002-0665-9

Cited by 9 publications

(3 citation statements)

References 24 publications

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“…Previously, when serological techniques were used to detect Cryptosporidium infection rates of 30.4% (61/201) in Gonghe and 36.8% (82/223) in Qilian were found. We detected a lower prevalence with microscopic diagnosis (20.2% in Gonghe) or PCR (28.2% in Qilian) [20], in agreement with a previous report that serological tests have a higher prevalence rate than microscopic or PCR-based diagnosis [29]. The infection rates of 36.2% (85/235) found in Huangyuan was higher than that found in a previous study which utilized microscopic diagnoses and found an infection rate of 30.6% (26/85) [30].…”

Section: Discussionsupporting

confidence: 92%

Prevalence and Genetic Characterization of Cryptosporidium in Yaks in Qinghai Province of China

Wang

Li³

et al. 2013

PLoS ONE

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The objective of this study was to determine the prevalence, species and subtypes of Cryptosporidium infecting yaks in the Qinghai Province of Northwestern China. The prevalence of Cryptosporidium spp. was detected by microscopy and nested-PCR. A total of 586 fecal samples were collected from yaks in 6 counties, of which 142 (24.2%) samples tested positive for Cryptosporidium. The small subunit (SSU) rRNA gene of fifty-five samples were amplified and sequenced successfully and demonstrated that Cryptosporidium bovis (31/55, 56.4%) was the most common species, followed by C. parvum (16/55, 29.1%) and C. ryanae (5/55, 9.0%). Mixed infections of C. parvum and C. bovis (n = 2), C. ryanae and C. bovis (n = 1) were also detected. All three species were found in yaks ranging in age from <1 year, 1–2 years, to >2 years. Cryptosporidium was most commonly detected in spring (28.4%), followed by summer (20.9%), then winter (17.5%). Cryptosporidium parvum positive samples were subtyped using the 60 kDa glycoprotein (gp60) gene. Subtypes IIaA15G2R1 (n = 8), IIaA16G2R1 (n = 2), IIaA14G1R1 (n = 1), IIaA14G2R1 (n = 1) and IIaA16G3R1 (n = 1) were detected. All of these subtypes are zoonotic, and may pose a potential threat to human health.

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Section: Discussionsupporting

confidence: 92%

Prevalence and Genetic Characterization of Cryptosporidium in Yaks in Qinghai Province of China

Wang

Li³

et al. 2013

PLoS ONE

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“…Given there was little agreement between serum ELISA and fecal tests on samples collected from adult cows [17], serological assays should be used to support fecal examinations. The reported infection rate of randomly selected inhabitants in Seoul was only 0.5% [18], whereas another report identified that the majority (94.6%) of oocyst-positive cases were people over 50 yr of age [19].…”

Section: Discussionmentioning

confidence: 99%

Antibody Responses to Cryptosporidium Antigen in HIV-positive Patients in the Republic of Korea

et al. 2008

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Abstract:The diagnosis of cryptosporidiosis has been carried out using coprologic techniques in the Republic of Korea. However, antibody responses to Cryptosporidium have rarely been studied. Serum antibodies from HIV-positive/oocyst-positive Korean patients recognized significantly 31 and 27 kDa antigens, and HIV-negative/oocyst-positive individuals clearly reacted to 15/17 kDa antigens. Compared with oocyst-positive cases, 18.7% and 75.8% of sera from HIV-positive patients reacted to 31 and 27 kDa antigens. Only 11.1% of HIV-negative individuals reacted to 15/17 kDa. Based on these findings, serum antibody responses were different between HIV-positive and HIV-negative individuals infected with Cryptosporidium, and it is suggested that HIV-positive patients are more frequently exposed to C. parvum compared to HIV-negative individuals.

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“…The serological study by ELISA had a high sensitivity and low specificity [13]. In the present study, the infection of C. parvum was investigated by using an ELISA technique.…”

Section: Discussionmentioning

confidence: 99%

Concurrent response to challenge infection withCryptosporidium parvumin immunosuppressed C57BL/6N mice

Surl

Kim

2006

J Vet Sci

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We investigated the response to challenge infection with Cryptosporidium parvum oocysts in immunosuppressed C57BL/6N mice. In the primary infection, fecal oocyst shedding and parasite colonization were greater in immunosuppressed mice than in nonimmunosuppressed mice. Compared with primary infection, challenge infection with C. parvum didn't show any oocyst shedding and parasite colonization. Especially, oocyst shedding and parasite colonization from the mice infected with heat-killed oocysts were not detected. After challenge infection with C. parvum oocysts, however, these mice were shedding small numbers of oocysts and parasite colonization. Except normal control and uninfected groups, the antibody titers of other groups appear similar. Based on the fecal oocyst shedding, parasite colonization of ilea, and antibody titers in the mice, these results suggest that the resistance to challenge infection with C. parvum in immunosuppressed C57BL/6N mice has increased.

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Serological detection of exposure to Cryptosporidium parvum in cattle by ELISA and its evaluation in relation to coprological tests

Cited by 9 publications

References 24 publications

Prevalence and Genetic Characterization of Cryptosporidium in Yaks in Qinghai Province of China

Prevalence and Genetic Characterization of Cryptosporidium in Yaks in Qinghai Province of China

Antibody Responses to Cryptosporidium Antigen in HIV-positive Patients in the Republic of Korea

Concurrent response to challenge infection withCryptosporidium parvumin immunosuppressed C57BL/6N mice

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