Serological detection of Toxoplasma gondii, Leishmania infantum and Neospora caninum in cats from an area endemic for leishmaniasis in Brazil

Sousa, Keyla Carstens Marques de; Herrera, Heitor Miraglia; Domingos, Iara Helena; Campos, João Bosco Vilela; Santos, Isabela Maria Campanelli dos; Neves, Haroldo Henrique de Rezende; Machado, Rosangela Zacarias; André, Marcos Rogério

doi:10.1590/s1984-29612014078

Cited by 29 publications

(24 citation statements)

References 45 publications

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“…The FeL seropositivity here was higher than that observed in Recife and Teresina, where 3.9% and 4% of the cats were found be seropositive for FeL using ELISA (SILVA et al, 2014;MENDONÇA et al, 2017). Sero-epidemiological investigations in other regions of the country found frequencies varying from 0.5% to 54%, using IFAT and the same cut-off value adopted here (1:40) (VIDES et al, 2011;CARDIA et al, 2013;BRAGA et al, 2014;SOUSA et al, 2014;OLIVEIRA et al, 2015;GODOI et al, 2016;COURA et al, 2018;MATOS et al, 2018). In canine leishmaniasis, high serological titers (four times the established cut-off value) with clinical signs compatible with leishmaniasis are diagnostic for the disease (SOLANO-GALLEGO et al, 2011).…”

Section: Introductionmentioning

confidence: 39%

“…In Brazil, studies on FeL using serological, parasitological, and molecular methods revealed that infection frequencies varied from 0% to 54% (BRESCIANI et al, 2010;VIDES et al, 2011;CARDIA et al, 2013;BRAGA et al, 2014;SILVA et al, 2014;SOUSA et al, 2014;OLIVEIRA et al, 2015;GODOI et al, 2016;MENDONÇA et al, 2017;COURA et al, 2018;MATOS et al, 2018). Only a small fraction of these studies was performed in the Northeast region that was home to 82.5% of all reported cases of human leishmaniasis in Brazil between 1980 and 2005, making this region an area of epidemiological relevance for this disease in Brazil (MAIA-ELKHOURY et al, 2008;SILVA et al, 2014;MENDONÇA et al, 2017).…”

Section: Introductionmentioning

confidence: 99%

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Serological and molecular investigation of Leishmania spp. infection in cats from an area endemic for canine and human leishmaniasis in Northeast Brazil

Bezerra

Oliveira

Yamakawa

et al. 2019

Rev. Bras. Parasitol. Vet.

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The aim of this study was to investigate the occurrence of Leishmania spp. antibodies, and its association with feline immunodeficiency virus (FIV) and feline leukemia virus (FeLV), in domestic cats from an area endemic for canine and human leishmaniasis in Rio Grande do Norte State, Brazil. Ninety-one cats were subjected to a complete clinical exam, and blood samples were collected. An epidemiological questionnaire was used to investigate the risk factors. IgG anti-Leishmania spp. antibodies were detected by immunofluorescence antibody test (IFAT), with a cut-off value of 1:40. Polymerase chain reaction (PCR) was performed to detect genetic material of Leishmania spp. in the blood samples. The presence of antibodies against FIV and antigens of FeLV was evaluated using an immunochromatographic test. Seropositivity for Leishmania spp., FIV, and FeLV was observed in 14/91 (15.38%), 26/91 (28.57%), and 3/91 (3.29%) cats, respectively. All samples gave negative results on PCR analysis. Based on these data, no significant statistical association was observed between seropositivity for Leishmania spp., and sex, age, presence of clinical signs, evaluated risk factors, and positivity for retroviruses. These findings demonstrated for the first time that cats from Mossoró, Rio Grande do Norte, are being exposed to this zoonosis and might be part of the epidemiological chain of transmission of visceral leishmaniasis.

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Section: Introductionmentioning

confidence: 39%

Section: Introductionmentioning

confidence: 99%

Serological and molecular investigation of Leishmania spp. infection in cats from an area endemic for canine and human leishmaniasis in Northeast Brazil

Bezerra

Oliveira

Yamakawa

et al. 2019

Rev. Bras. Parasitol. Vet.

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“…Ten microliters of two fold dilutions of sera (cut-off of 1:40) were placed in wells on antigen slides. Known positive serum samples for the studied agents were obtained from naturally infected deer [26] and dogs [27] from Brazil. Negative serum samples were obtained from wild deer captured in Brazil [26], and dogs maintained in the kennel of the Department of Veterinary Pathology, UNESP, Jaboticabal, São Paulo, Brazil, that had not been exposed to these agents, according to negative PCR and IFAT results.…”

Section: Serology For Babesia Vogeli Anaplasma Phagocytophilum Ehrlmentioning

confidence: 99%

Arthropod-borne agents in wild Orinoco geese ( Neochen jubata ) in Brazil

Werther

Luzzi

Gonçalves

et al. 2017

Comparative Immunology, Microbiology and Infectious Diseases

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Although Orinoco goose (Neochen jubata) is an anatid species widely distributed in South America, scarce are the reports on the occurrence of arthropod-borne pathogens in this avian species. The present work aimed to verify, by serological and molecular methods, the occurrence of haemosporida piroplasmids and Anaplasmataceae agents in wild Orinoco geese captured in Brazil. Between 2010 and 2014, 62 blood samples were collected from free-living geese captured in the Araguaia River, Goiás State, Brazil. Six geese (10%) were seropositive for Anaplasma phagocytophilum, showing titers ranging from 40 and 80. Twenty out of 62 blood samples (32.25%) were positive in nested PCR for hemosporidia (cytochrome b gene). Fifteen and five sequences shared identity with Haemoproteus and Plasmodium, respectively. Six out of 62 blood samples (9.68%) were positive in nested PCR for Babesia spp. (18S rRNA gene); one sequence showed to be closely related to Babesia vogeli. Thirty (48.38%) out of 62 Orinoco geese blood samples were positive in nested cPCR assays for Anaplasmataceae agents (16S rRNA gene): three for Anaplasma spp. and 27 for Ehrlichia. Six geese were simultaneously positive to Haemoproteus and Ehrlichia; three animals were co-positive to different Ehrlichia species/genotypes; and one goose sample was positive for both Anaplasma and Ehrlichia. The present work showed the occurrence of Ehrlichia, Anaplasma, Babesia, Plasmodium, and Haemoproteus species in free-living N. jubata in Brazil. The threat of these arthropod-borne pathogens in Orinoco goose's fitness, especially during the breading season, should be assessed in the future.

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“…In dogs, T. gondii generally does not cause primary infection unless preceded by an immunosuppressive disease [33]. A significant association has previously been found in several studies between L. infantum and T. gondii in dogs, suggesting that the immunosuppression caused by L. infantum may enhance the susceptibility of dogs to T. gondii [31,32,34]. However, this hypothesis lacks scientific corroboration.…”

Section: Similar Findings Have Been Reported By Other Researchersmentioning

confidence: 99%

Seroepidemiological study of canine <i>Leishmania infantum</i> and <i>Toxoplasma gondii</i> infections in Shanghai, China, and analysis of risk factors

Jiang¹,

Wang²,

Liu³

et al. 2016

Ann Agric Environ Med.

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The aim of this study was to determine the seroprevalence of Leishmania infantum and Toxoplasma gondii among household dogs in Shanghai (the most important industrial and commercial city in China), and to assess the possible risk factors associated with the infection. During 2014-2015, a total of 408 sera were collected from healthy household dogs and tested for L. infantum and T. gondii infection using commercial ELISA kits. The endemic characteristics according to gender, age group and breed were revealed by statistical descriptions and inference. The positive rates of L. infantum infection (24/408, 5.9%) were lower than those of T. gondii infection (37/408, 9.1%), and co-infection with both parasites was detected in seven dogs (7/408, 1.7%). Seropositivity for either parasite was more likely associated with age: the seroprevalence of T. gondii infection ranged from 1.3% (dogs≤1 year) -18.7% (dogs>6 years), whereas that of L. infantum ranged from 1.3% (dogs≤1 year) -9.9 % (dogs>6 years). Interestingly, the rates of exposure to both L. infantum and T. gondii were higher in males than in females. Relatively higher exposure rates for L. infantum and T. gondii were also observed in crossbred dogs compared with purebred dogs. However, neither gender nor breed is likely a determining factor for infection with these two parasites (P > 0.05). Identification of the risk factors that underlie these differences may help in the prevention of L. infantum and T. gondii infection in household dogs. To the best of our knowledge, this is the first report of L. infantum and T. gondii infection in household dogs in Shanghai, which shows that these two important parasites are still prevalent in this region. Therefore, it is necessary to take integrated strategies for prevention and control of infection in animals, which could help to reduce human infection in the region. et al. Seroepidemiological study of canine Leishmania infantum and Toxoplasma gondii …

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Serological detection of Toxoplasma gondii, Leishmania infantum and Neospora caninum in cats from an area endemic for leishmaniasis in Brazil

Cited by 29 publications

References 45 publications

Serological and molecular investigation of Leishmania spp. infection in cats from an area endemic for canine and human leishmaniasis in Northeast Brazil

Serological and molecular investigation of Leishmania spp. infection in cats from an area endemic for canine and human leishmaniasis in Northeast Brazil

Arthropod-borne agents in wild Orinoco geese ( Neochen jubata ) in Brazil

Seroepidemiological study of canine <i>Leishmania infantum</i> and <i>Toxoplasma gondii</i> infections in Shanghai, China, and analysis of risk factors

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