Serological diagnosis of erythema migrans disease and related disorders

Wilske, Bettina; Schierz, G; Preac-Mursic, V.; Weber, Klaus; Pfister, Hans‐Walter; Einhäupl, Karl M.

doi:10.1007/bf01651147

Cited by 144 publications

(55 citation statements)

References 13 publications

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“…Hansen et al (1988) assumed that serological tests using whole-cells antigens are of low diagnostic specificity. Several strategies for increasing both sensitivity and specificity have been developed, such as preabsorbtion of cross-reactive antibodies with Treponema phagadensis (Wilske et al 1984), the use of detergent extracts of Borrelia burgdorferi s.l. (Bergström et al 1991) and the use of purified flagella or various recombinant antigens (Hansen et al 1991;Lenčáková et al 2006).…”

Section: Discussionmentioning

confidence: 99%

Some epidemiological and epizootiological aspects of Lyme borreliosis in Slovakia with the emphasis on the problems of serological diagnostics

et al. 2008

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The paper analyses the results of serological examinations of domestic, farm and free-living animals from different regions of Slovakia, Southern Moravia, Southern Bohemia and Southern Poland using ELISA, indirect hemagglutination assay (IHA) and Western blot (WB). In Slovakia, significantly higher seroprevalence was recorded in dogs (33.5%) than in horses (26.5%), cattle (22.5%), sheep (16.6%) and rodents (17.8%) by using a mixture of Borrelia garinii, B. afzelii, B. burgdorferi sensu stricto (s.s.) antigens in ELISA. Seroprevalence in horses was significantly higher than in sheep and rodents, and seroprevalence in cattle was significantly higher than in rodents. By using IHA in free-living species, the highest seropositivity rates were detected in fallow deer (40.7%) compared with moufflons (16.6%), pheasants (8.0%) and pigeons (1.2%). When testing sera of horses, dogs and cattle from Slovakia by using different Slovak B. burgdorferi sensu lato (s.l.) isolates as antigens in ELISA, significantly higher seroprevalence of anti-Borrelia IgG antibodies and consistency of positive and negative findings was detected in comparison when American isolates were used. In WB analyses using the Eurocarpathian antigens, dog sera from Eastern Slovakia and Southern Moravia showed statistically insignificant differences in sensitivity and consistency of positive and negative findings. By using different methods and antigens in the same group of dog sera, significant differences in seroprevalence were only found in IHA with a mixture of Euroamerican B.b.s.l and WB CB26 B.b.s.s. In addition to other factors, the complexity of the standardization of the assay system with regard to the genetic and geographical heterogeneity of B. burgdorferi s.l. isolates used as antigens is also discussed.

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Section: Discussionmentioning

confidence: 99%

Some epidemiological and epizootiological aspects of Lyme borreliosis in Slovakia with the emphasis on the problems of serological diagnostics

et al. 2008

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“…Another factor that lowers the titer and is considered diagnostic is whether a "sorbent" material is used. In most reports the adsorbing material was derived from Treponema phagedenis biotype Reiter cells (2, 54,89,126,128,130,177,178). While the adsorption step reduces antibody titers to both B. burgdorferi and T. phagedenis in Lyme disease patients, the magnitude of the drop is greater against T. phagedenis.…”

Section: Differential Diagnosismentioning

confidence: 99%

Laboratory aspects of Lyme borreliosis

Barbour

1988

Clin Microbiol Rev

130

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Lyme borreliosis (Lyme disease), a common tick-borne disorder of people and domestic animals in North America and Europe, is caused by the spirochete Borrelia burgdorferi. Following the discovery and initial propagation of this agent in 1981 came revelations that other tick-associated infectious disorders are but different forms of Lyme borreliosis. A challenge for the clinician and microbiology laboratory is confirmation that a skin rash, a chronic meningitis, an episode of myocarditis, or an arthritic joint is the consequence of B. burgdorferi infection. The diagnosis of Lyme borreliosis may be established by (i) directly observing the spirochete in host fluid or tissue, (ii) recovering the etiologic spirochete from the patient in culture medium or indirectly through inoculation of laboratory animals, or (iii) carrying out serologic tests with the patient's serum or cerebrospinal fluid. The last method, while lacking in discriminatory power, is the most efficacious diagnostic assay for most laboratories at present.

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“…These include the use of detergent extracts to isolate proteins of interest by molecular weight (9), preabsorption of sera with other bacteria to eliminate cross-reactive antibodies (64), and the use of purified proteins (22). More recently, third-generation serologic tests using sensitive synthetic peptides and recombinant proteinbased techniques have been reported (23,35,36).…”

mentioning

confidence: 99%

Evaluation of the Recombinant VlsE-Based Liaison Chemiluminescence Immunoassay for Detection ofBorrelia burgdorferiand Diagnosis of Lyme Disease

Ledue

Collins

Young

et al. 2008

Clin Vaccine Immunol

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Recent efforts to improve the serologic diagnosis of Lyme disease have included the use of a synthetic peptide (C6) that reproduces the sequence of invariable region 6 of VlsE, the variable surface antigen of Borrelia burgdorferi. In the present study, the diagnostic performance of DiaSorin's recombinant VlsE-based chemiluminescence immunoassay in 1,947 human serum samples was evaluated. Sensitivity was determined using two serum panels from the CDC. For panel I, we observed sensitivities of 68.4% and 75.6% for subjects with early, localized (n ‫؍‬ 19) or disseminated (n ‫؍‬ 41) disease, respectively. For panel II, we observed sensitivities of 61.5% and 100% for subjects with early (n ‫؍‬ 26) or late-stage (n ‫؍‬ 11) disease, respectively. We observed a specificity of 99.5% for healthy donors (n ‫؍‬ 600) living either in regions of the United States where the disease is endemic or in regions where it is not endemic. Overall, specificity among 207 potentially cross-reactive sera from subjects who had other spirochetal infections, nonspirochetal infections including bacterial and viral infections, or autoimmune or neurologic disease; who were positive for rheumatoid factor or anti-mouse antibodies; or who had been previously vaccinated for Lyme disease was 93.7%. In a direct comparison of 1,038 prospectively collected samples for Lyme disease testing we observed a relative sensitivity of 70%, a relative specificity of 99.1%, and an overall agreement of 97.1% between the DiaSorin recombinant VlsE chemiluminescence immunoassay and the Immunetics peptide-based C6 enzyme-linked immunosorbent assay.

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Serological diagnosis of erythema migrans disease and related disorders

Cited by 144 publications

References 13 publications

Some epidemiological and epizootiological aspects of Lyme borreliosis in Slovakia with the emphasis on the problems of serological diagnostics

Some epidemiological and epizootiological aspects of Lyme borreliosis in Slovakia with the emphasis on the problems of serological diagnostics

Laboratory aspects of Lyme borreliosis

Evaluation of the Recombinant VlsE-Based Liaison Chemiluminescence Immunoassay for Detection ofBorrelia burgdorferiand Diagnosis of Lyme Disease

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