Serological diagnosis of parainfluenza virus infections by enzyme immunoassay with special emphasis on purity of viral antigens

Julkunen, Ilse

doi:10.1002/jmv.1890140212

Cited by 60 publications

(35 citation statements)

References 19 publications

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“…These results were similar to those of Julkunen (1984) who found that hyperimmune sera against the envelope glycoproteins of mumps virus and parainfluenza type 3 virus did not cross-react with the heterologous virus. In the present study, nine monoclonal antibodies directed against Sendai virus reacted either with mumps virus or parainfluenza type 3 virus, but no monoclonal antibody against Sendai virus reacted with both heterologous viruses simultaneously.…”

Section: Reactivity Of Eight Monoclonal Antibodies Directed Against Dsupporting

confidence: 90%

“…antibodies can be employed in a particular study. Intertypic antigenic relationships between paramyxoviruses have been studied previously both with monoclonal (Goswami & Russell, 1983;Ray & Compans, 1986) and polyclonal sera (Goswami & Russell, 1982;Julkunen, 1984). In the present study, three mumps virus monoclonal antibodies directed against different structural proteins reacted with Sendai virus.…”

Section: Reactivity Of Parainfluenza Type 3 Virus Monoclonal Antibodimentioning

confidence: 70%

“…In the present study, three mumps virus monoclonal antibodies directed against different structural proteins reacted with Sendai virus. Immunological reactivity between Sendai virus and antibodies against mumps virus was also described by Julkunen (1984). A reciprocal immunological relationship existed between the two viruses, as six monoclonal antibodies against the HN protein and one against the NP protein of Sendai virus reacted with mumps virus.…”

Section: Reactivity Of Parainfluenza Type 3 Virus Monoclonal Antibodimentioning

confidence: 97%

“…In fact, it was found that hyperimmune sera against purified Sendai virions could precipitate the M protein of parainfluenza type 3 virus (C. Orvell, unpublished data). An antigenic relationship between the HN and NP proteins of Sendai and parainfluenza type 3 viruses has been described previously (Goswami & Russell, 1982Julkunen, 1984;Ray & Compans, 1986). From cloning experiments it has been deduced that the HN proteins of Sendai virus and parainfluenza type 3 virus show similarities in the amino acid sequence of a hydrophobic region near the N terminus of the protein (Elango et al, 1986;Blumberg et al, 1985).…”

Section: Reactivity Of Eight Monoclonal Antibodies Directed Against Dmentioning

confidence: 99%

“…In a recent study using a large collection of monoclonal antibodies directed against the HN glycoprotein of parainfluenza 3 virus, a few antibodies were found to react with Sendal virus (Ray & Compans, 1986). In another recent study, Julkunen (1984) reported marked cross-reactivity between rabbit hyperimmune sera against parainfluenza type 1 and 3 viruses in tests with both purified envelope components and nucleocapsid preparations of the two viruses. In the same study, it was also reported that antibodies against mumps virus reacted with both the HN and fusion (F) envelope glycoproteins of parainfluenza type 1 virus.…”

Section: Introductionmentioning

confidence: 99%

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Immunological Relationships between Mumps Virus and Parainfluenza viruses Studied with Monoclonal Antibodies

Örvell¹,

Rydbeck

Löve

1986

Journal of General Virology

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SUMMARYThe immunological relationships between mumps virus and parainfluenza viruses were investigated with 74, 78 and 80 previously developed monoclonal antibodies directed against five major structural proteins of mumps virus, Sendal virus (a murine parainfluenza type 1 virus) and parainfluenza type 3 virus. These monoclonal antibodies were reacted with the three viruses, with parainfluenza type 2 virus and with Newcastle disease virus,(NDV) in ELISA and immunofluorescence (IF) tests. In addition, immunoprecipitation tests with [35S]methionine-labelled extracellular virions were carried out with cross-reacting monoclonal antibodies. None of all 232 monoclonal antibodies against the three viruses cross-reacted with either parainfluenza type 2 virus or NDV in ELISA and IF tests. In the collection of 74 mumps virus monoclonal antibodies, three directed against the nucleocapsid (NP) protein, polymerase protein, and fusion protein cross-reacted with Sendal virus. Two Sendal virus monoclonal antibodies directed against two different epitopes of the haemagglutinin-neuraminidase (HN) protein cross-reacted with parainfluenza type 3 virus. Six other Sendai virus monoclonal antibodies directed against four different epitopes of the HN protein and one directed against the NP protein cross-reacted with mumps virus. Eight out of 80 monoclonal antibodies directed against parainfluenza type 3 virus crossreacted with Sendal virus. One was directed against the HN protein, four were directed against a minimum of two epitopes of the matrix protein and three were directed against three different epitopes of the NP protein. The different cross-reactions found show that Sendai virus is antigenically related to both mumps virus and parainfluenza type 3 virus, In contrast, no antigenic relationship could be demonstrated between mumps virus and parainfluenza type 3 virus.

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Section: Reactivity Of Eight Monoclonal Antibodies Directed Against Dsupporting

confidence: 90%

Section: Reactivity Of Parainfluenza Type 3 Virus Monoclonal Antibodimentioning

confidence: 70%

Section: Reactivity Of Parainfluenza Type 3 Virus Monoclonal Antibodimentioning

confidence: 97%

Section: Reactivity Of Eight Monoclonal Antibodies Directed Against Dmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Immunological Relationships between Mumps Virus and Parainfluenza viruses Studied with Monoclonal Antibodies

Örvell¹,

Rydbeck

Löve

1986

Journal of General Virology

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Rapid and sensitive detection of mumps virus RNA directly from clinical samples by real-time PCR

Uchida

Shinohara²,

Shimada³

et al. 2005

J. Med. Virol.

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A rapid, sensitive, and specific assay to detect mumps virus RNA directly from clinical specimens using a real-time PCR assay was developed. The assay was capable of detecting five copies of standard plasmid containing cDNA from the mumps virus F gene. No cross-reactions were observed with other members of Paramyxoviridae, or with viruses or bacteria known to be meningitis pathogens. Seventy-three clinical samples consisting of throat swabs collected from patients with parotitis, and cerebrospinal fluid (CSF) collected from patients with aseptic meningitis, were examined with a real-time PCR assay developed by the authors, reverse-transcription nested-PCR (RT-n-PCR), and virus isolation using cell culture. Like the RT-n-PCR assay, the real-time PCR assay could detect mumps virus RNA in approximately 70% of both throat swabs and CSF samples, while, by tissue culture, mumps virus was isolated from only approximately 20% of CSF and 50% of throat swab samples. In addition, the real-time PCR assay could be developed easily into a quantitative assay for clinical specimens containing more than 1,800 copies of mumps virus RNA/ml by using serial dilutions of the standard plasmid. The results suggest that the real-time PCR assay is useful for identification of mumps virus infections, not only in typical cases, but also in suspected cases, which show only symptoms of meningitis or encephalitis.

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Determination of Antibody to Respiratory Pathogens

Schwartzman¹,

Kamforin²

1989

Reviews on Immunoassay Technology

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Qualitative evaluation and quantitative determination of antibody to respiratory pathogens represents a substantial part of research and applied virology. These investigations can serve quite different purposes. Epidemiologists need the serological data necessary for the control of influenza and other respiratory infections and for elucidating aetiological peculiarities of past infections. A system of constant surveillance of influenza morbidity and seroepidemiological status of the population has been organized in the USSR. The herd immunity is assessed as to circulating influenza viruses and some older strains which have gone out of circulation. The results obtained are important not only for current epidemiological analysis, but also because they enable the prognosis, to some extent, of the epidemic process, the evaluation of danger in shift of influenza viruses when their reappearance in the circulation is anticipated, as was the case in 1976 in the USA and in 1977 in China and in the USSR.Specialists who are developing new vaccines or improving existing ones are interested in determining the immunological status of those vaccinated. These results are essential for immunologists in order to elucidate mechanisms of humoral immunity. Based on serological information, the impact of chemoprophylactic and chemotherapeutic drugs on the immunological system can be evaluated. Determination of immune response to an infectious agent contributes essentially to a reliable diagnosis; as a result, the improvement of serological techniques is perpetuated in further investigations. It goes without saying that neither a single technique nor even a limited number of them can provide a solution to all the problems mentioned above.The purpose of this review is consideration of the main serological techniques available and their application in serological investigations of acute respiratory infections.

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Serological diagnosis of parainfluenza virus infections by enzyme immunoassay with special emphasis on purity of viral antigens

Cited by 60 publications

References 19 publications

Immunological Relationships between Mumps Virus and Parainfluenza viruses Studied with Monoclonal Antibodies

Immunological Relationships between Mumps Virus and Parainfluenza viruses Studied with Monoclonal Antibodies

Rapid and sensitive detection of mumps virus RNA directly from clinical samples by real-time PCR

Determination of Antibody to Respiratory Pathogens

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