Serological Diagnosis of Toxoplasma Gondii Infection in Various Patient Population in the Armed Forces

Abstract

The study included 200 healthy age and sex matched controls (120 healthy volunteers and 80 healthy antenatal cases without any bad obstetric history) and 300 cases comprising patients with bad obstetric history (BOH) 80 cases, seizure or encephalitis 50 cases, cervical lymphadenopathy 30 cases, congenital deformity 30 cases, HIV infected 80 cases and patients on long term immunosuppression 30 cases. Anti-toxoplasma IgG & Igl\l were measured by ELISA and lndivlduals found positive for anti Toxoplasma gondii IgM or with high titre of IgG were subjected to repeat testing after 3 weeks for rise in titre. True sero-prevalence of T gondii infection was found to be 19% and it increased with increasing age in the population, 2.33% patients were found to have acute toxoplasma infection. Statistical significance was seen only in patients with BOH and children born with congenital abnormality. MJAFI 2001; 57: 298-301KEY WORDS: Bad obstetric history; Congenital abnormality; Serodiagnosis; Toxoplasmosis. 5 ml.of blood was collected and serum after separation was stored at -70 uC for testing. Serum was assayed for IgG and IgM antibody to T gondii by using commercial ELISA kits (United Biotech U.S.A). All the samples were tested in duplicate alongwith kit controls and the mean value of each sample was taken into consideration for calculating results. Manufacturer's instruction was followed for ELISA procedure. Serum samples from patients were re-tested for antitoxoplasma IgG antibody after 3 weeks if found positive for IgM isotype or with high titre of IgG antibody in the first testing. Statistical analysis was carried out by Chisquare test and odds ratio.

Toxoplasmosis Retinochoroiditis Masquerading as Endogenous Endophthalmitis in a Case of Congenital Long Qt Syndrome

“…3 There are, however, reports of marked increases in toxoplasma IgG titers greater than 4-fold above normal limits in cases of acute toxoplasmosis infection. 7 Although it is unclear why our patient's toxoplasma IgM and polymerase chain reaction studies were negative, her ophthalmic examination, multimodal imaging findings, history of travel and high-risk exposure, and positive response to antiparasitic therapy ultimately supported our clinical diagnosis of ocular toxoplasmosis.…”

Congenital Toxoplasmosis: An Overview on Transmission, Diagnosis and Treatment With Reference to Egypt

“…More specific confirmatory tests was used, like a dye test or immunoblotting (Lesle et al, 2011). Praharaj et al (2001) in India reported bad obstetric outcome (patients with history of repeated abortions, still births and giving birth to babies with congenital abnormalities) was one of the commonest presentations of Toxoplasma infection during pregnancy. Nogareda et al (2014) in France reported that repeated monthly testing continues during pregnancy in high-risk was a must.…”