Serological evidence of equine infectious anaemia, West Nile fever, surra and equine piroplasmosis in a herd of horses in northern Argentina

Hébert, Laurent; Polledo, Gonzalo; Lecouturier, Fanny; Giorgi, Mariana; Beck, Cécile; Lowenski, Steeve; Laroucau, Karine; Büscher, Philippe; Hans, Aymeric; Becú, Teótimo

doi:10.1016/j.vprsr.2021.100566

Cited by 3 publications

(3 citation statements)

References 17 publications

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“…Of this total, 164 sera were collected between January 2015 and December 2017 in France (a region of France where dourine, surra and nagana were not recorded over the last 40 years), 99 were seropositive for equine piroplasmosis, 5 seropositive for equine viral arteritis, 3 seropositive for equine infectious anaemia and 5 seropositive for equine herpesvirus. Seven sera were from a sampling campaign performed in Argentina 33 and 18 from a study involving the experimental infection of pony mares 34 , 35 .…”

Section: Methodsmentioning

confidence: 99%

Development of a microsphere-based immunoassay for the serological diagnosis of equine trypanosomosis

Verney

Gautron

Lemans

et al. 2022

Sci Rep

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Trypanozoon infections in equids are caused by three parasite species in the Trypanozoon subgenus: Trypanosoma equiperdum, T. brucei and T. evansi. They are respectively responsible for infectious diseases dourine, nagana and surra. Due to the threat that Trypanozoon infection represents for international horse trading, accurate diagnostic tests are crucial. Current tests suffer from poor sensitivity and specificity, due in the first case to the transient presence of parasites in the blood and in the second, to antigenic cross-reactivity among Trypanozoon subspecies. This study was designed to develop a microsphere‐based immunoassay for diagnosing equine trypanosomosis. We tested beads coated with eight Trypanosoma spp. recombinant antigens: enolase, GM6, PFR1, PFR2, ISG65, VSGat, RoTat1.2 and JN2118HU. Of these, GM6 was identified as the best candidate for the serological diagnosis of Trypanozoon infections in equids. Using a receiver operating characteristic (ROC) analysis on 349 equine sera, anti-GM6 antibodies were detected with an AUC value of 0.994 offering a sensitivity of 97.9% and a specificity of 96.0%. Our findings show that the GM6 antigen is a good target for diagnosing equine trypanosomosis using a microsphere‐based immunoassay. This promising assay could be a useful alternative to the official diagnostic tool for equine trypanosomosis.

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Section: Methodsmentioning

confidence: 99%

Development of a microsphere-based immunoassay for the serological diagnosis of equine trypanosomosis

Verney

Gautron

Lemans

et al. 2022

Sci Rep

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“…EIA virus (EIAV) infection leads to recurring episodes of fever in equids, thrombocytopenia, and wasting symptoms. EIA has been detected in different regions in Brazil ( 12–14 ) and Argentina ( 15 ) and several outbreaks were reported in different regions in mainland Ecuador ( 16 ).…”

Section: Introductionmentioning

confidence: 99%

Undetection of vector-borne viruses in equids of Galapagos Islands

Zanella,

Beck,

Valle-Casuso

et al. 2024

Front. Vet. Sci.

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Domestic species, including equids, were introduced in the Galapagos Islands in the XIX century. Equine vector-borne diseases are circulating in South America but their occurrence in the Galapagos Island was unknown. The objective of this study was to detect the occurrence of West Nile virus (WNV), Usutu virus (USUV) and equine infectious anemia virus (EIAV) in the four Galapagos Islands raising equids if they were present at a prevalence >1%. Serum samples were collected from 411 equids belonging to 124 owners from April to July 2019. All the results were negative to the ELISA tests used suggesting that WNV, USUV and EIAV are not circulating in the equine population of the Galapagos Islands.

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“…Currently, equine trypanosomosis in general and dourine in particular remain endemic in parts of Africa [8,9], Asia [10,11] and Latin America [12][13][14]. Apart from sporadic cases [15,16], many parts of the world are considered free of equine trypanosomosis, including Europe and North America [17].…”

Section: Introductionmentioning

confidence: 99%

European Inter-Laboratory Proficiency Test for Dourine Antibody Detection Using the Complement Fixation Test

Hébert,

Froger,

Madeline

et al. 2023

Veterinary Sciences

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Dourine is a sexually transmitted parasitic disease affecting equids. Its causative agent is referred to as Trypanosoma equiperdum and the prescribed serodiagnosis method is the complement fixation test (CFT). In the context of our European Reference Laboratory mandate for equine diseases (excluding African horse sickness), we organised dourine CFT inter-laboratory proficiency tests (ILPTs) in 2015, 2018 and 2022 to evaluate the performance of the European Union network of National Reference Laboratories (NRLs) for dourine. ILPT panels were composed of horse sera with or without antibodies against Trypanosoma spp. originating from non-infected, immunised or experimentally infected horses. Twenty-two NRLs participated in at least one of the three sessions. In 2015, 2018 and 2022, the percentage of laboratories obtaining 100% of the expected results was 57, 90 and 80, respectively. These dourine CFT ILPTs showed the benefits of standardising the method’s detection limit and underlined the constant need to evaluate NRLs to improve the network’s performance. These results also argue in favour of the need for a representative bio-bank to improve the representativeness of ILPT samples and to allow the adoption of alternative serological methods for international surveillance of dourine.

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Serological evidence of equine infectious anaemia, West Nile fever, surra and equine piroplasmosis in a herd of horses in northern Argentina

Cited by 3 publications

References 17 publications

Development of a microsphere-based immunoassay for the serological diagnosis of equine trypanosomosis

Development of a microsphere-based immunoassay for the serological diagnosis of equine trypanosomosis

Undetection of vector-borne viruses in equids of Galapagos Islands

European Inter-Laboratory Proficiency Test for Dourine Antibody Detection Using the Complement Fixation Test

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