Serological follow-up of patients with paracoccidioidomycosis treated with itraconazole using Dot-blot, ELISA and Western-blot

Martins, Rosana Aparecida Rodrigues; Marques, Sílvio Alencar; Alves, M. A. B.; Fecchio, Denise; Franco, Marcello

doi:10.1590/s0036-46651997000500004

Cited by 30 publications

(25 citation statements)

References 14 publications

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“…Some authors attempted to demonstrate that there was an effective relationship between the clinical and serological characteristics during the evolution of PCM [56,57]. Campos et al [56] compared various serological tests, such as CF, ID, CIE, and Magnetic-ELISA (MELISA) to find out the most effective tool for monitoring patients under antimycotic therapy.…”

Section: Follow-up Of Pcm Patients Based On Antibody Detectionmentioning

confidence: 99%

Serology of paracoccidioidomycosis

Camargo

2008

Mycopathologia

114

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This review provides the background for understanding the role of a battery of diagnostic methods in paracoccidioidomycosis (PCM). This systemic mycosis is a disease endemic in many regions of Latin America, with sporadic cases also occurring throughout the world (mycosis of importation). Although excellent laboratory methods for diagnosis are available, there are deficiencies that must be met by continued research. Understanding the uses and limitations of a battery of laboratory methods is essential to diagnose PCM. Clinicians and laboratory directors must be familiar with the uses and limitations of a battery of serologic and mycological tests to accurately diagnose of PCM. Antibody and antigen detections are valuable adjuncts to histopathology and culture. More recently, the gp43 and gp70 antigen detection assay have improved the methodology of diagnosis of this mycosis, which improves reproducibility and facilitates monitoring antigen clearance during antifungal treatment. Furthermore, detection of antigen in cerebrospinal fluid and in bronchoalveolar lavage fluid increases the sensitivity for diagnosis of PCM in central nervous system and in pulmonary infections, respectively.

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Section: Follow-up Of Pcm Patients Based On Antibody Detectionmentioning

confidence: 99%

Serology of paracoccidioidomycosis

Camargo

2008

Mycopathologia

114

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“…Several serological tests for detection of anti-P. brasiliensis antibodies, such as complement fixation (14), double immunodiffusion (DID) (6), counterimmunoelectrophoresis (8), the enzyme-linked immunosorbent assay (ELISA) (11), and dot blot analysis (11,21), can be used in the diagnosis of PCM. However, none of these has the characteristics of high sensitivity and specificity needed for an accurate diagnosis (1,8,10).…”

mentioning

confidence: 99%

Serological Diagnosis of Paracoccidioidomycosis through a Western Blot Technique

Perenha-Viana

Gonzales

Brockelt

et al. 2012

Clin. Vaccine Immunol.

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ABSTRACTParacoccidioidomycosis (PCM) is a serious infectious disease that progresses toward death if untreated. Its confirmatory diagnosis is made by the detection of the fungusParacoccidioides brasiliensisin a direct mycological examination or by histopathology. However, these techniques are of low sensitivity. Serological tests seem to be more promising. The objective of this study was to test Western blot (WB) analysis using sera from patients suspected of PCM to determine whether it represents a safe and sensitive serological technique for a rapid and effective diagnosis for this disease. Sera from 517 patients were analyzed through WB analysis and double-immunodiffusion (DID) techniques using a crude exoantigen ofP. brasiliensis339. DID gave positive reactions for 140 sera (27%) and WB for 250 sera (48.4%). All sera that had a positive reaction by DID also had a positive result with a 43-kDa glycoprotein by WB analysis. Among the 377 samples that were negative by DID, 29.1% were reactive in WB analysis. For the cutoff dilution used (1:400), a positive reaction was not observed with any of the 102 sera from patients with other diseases in regions where such diseases are endemic and 30 healthy individuals tested as negative controls. These results prove WB analysis to be a sensitive technique and suggest its inclusion among routine laboratory assays as a safe method for PCM diagnosis.

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“…However, due to the time and material required to perform these techniques, serologic tests have been developed to detect both antibodies (7,19,30,31) and antigens (14,15,16,22). Serologic tests are important tools when other procedures are not available as well as for posttherapy follow-up (21). The immunodiffusion (ID) test is one of the most widely used techniques due to the simplicity and low costs involved in its execution.…”

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confidence: 99%

Negative Immunodiffusion Test Results Obtained with Sera of Paracoccidioidomycosis Patients May Be Related to Low-Avidity Immunoglobulin G2 Antibodies Directed against Carbohydrate Epitopes

Neves

Mamoni

Rossi

et al. 2003

Clin Vaccine Immunol

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Immunodiffusion (ID) is the serologic test most frequently used for the diagnosis and posttherapy follow-up of patients with paracoccidioidomycosis (PCM). The ID test is highly specific (100%), but its sensitivity is relatively low (90%), leading to false-negative results. The aim of this study was to determine the profiles of antibodies in sera from patients with proven PCM and with negative results in the ID test (ID neg ) versus positive results in the ID test (ID pos ). We analyzed 46 sera from patients with active PCM for total immunoglobulin G (IgG) and IgG subclass responses to Paracoccidioides brasiliensis gp43 antigen (treated or not treated with sodium metaperiodate) by enzyme-linked immunosorbent assay and immunoblotting. Immunoblotting showed that both ID neg and ID pos sera recognized predominantly the gp43 fraction of the P. brasiliensis antigen used in the ID test. ID neg sera contain low-avidity antibodies, low levels of specific IgG (total) and IgG1, and high levels of IgG2 compared with ID pos sera. The antibodies present in ID neg sera were predominantly directed against carbohydrate epitopes, since treatment with sodium metaperiodate resulted in a significant decrease in antibody reactivity. These data suggest that the lack of reactivity of sera from PCM patients in the ID test may be related to the production of low-avidity IgG2 antibodies directed against carbohydrate epitopes.

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Serological follow-up of patients with paracoccidioidomycosis treated with itraconazole using Dot-blot, ELISA and Western-blot

Cited by 30 publications

References 14 publications

Serology of paracoccidioidomycosis

Serology of paracoccidioidomycosis

Serological Diagnosis of Paracoccidioidomycosis through a Western Blot Technique

Negative Immunodiffusion Test Results Obtained with Sera of Paracoccidioidomycosis Patients May Be Related to Low-Avidity Immunoglobulin G2 Antibodies Directed against Carbohydrate Epitopes

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