Serological investigation of Gyrovirus homsa1 infections in chickens in China

Abstract

Background Gyrovirus homsa1 (GyH1) (also known as Gyrovirus 3, GyV3) is a non-enveloped, small, single-stranded DNA virus, which was first identified in children with acute diarrhea, and was subsequently detected in marketed chickens, broilers with transmissible viral proventriculitis (TVP), and mammals. GyH1 is a pathogenic virus in chickens, causing aplastic anemia, immunosuppression, and multisystem damage. However, the seroepidemiology of GyH1 infection in chickens remains unclear. Here, we investigated the seroprevalence of GyH1 in chickens by ELISA to reveal the endemic status of GyH1 in China. Results An indirect ELISA with high sensitivity and specificity was developed for investigation of seroepidemiology of GyH1 in chickens in China. The seropositive rate of GyH1 ranged from 0.6% to 7.7% in thirteen provinces, and ranged from 4.1% to 8.1% in eight species chickens. The seropositive rate of GyH1 in broiler breeders was significantly higher than that of in layers. There was a negative correlation between seropositive rate and age of chickens. The highest and lowest seropositive rate were present in chickens at 30–60 days and over 180 days, respectively. Conclusions The seroepidemiological investigation results demonstrated that natural GyH1 infection is widespread in chickens in China. Different species showed different susceptibility for GyH1. Aged chickens showed obvious age-resistance to GyH1. GyH1 has shown a high risk to the poultry industry and should be highly concerned.

Development of a DAS–ELISA for Gyrovirus Homsa1 Prevalence Survey in Chickens and Wild Birds in China

“…Importantly, GyH1 co–infection with CAV leads to higher mortality and more widespread lymphocyte apoptosis [ 19 ], which increases the risk posed and makes prevention and control more difficult. Specifically, epidemiology investigation suggests that GyH1 is widespread in China, which is consistent with our findings [ 17 , 18 , 20 ]. Thus, an accurate diagnosis of GyH1 is particularly important.…”

“…We found that the highest positive rate of GyH1 occurred in 2019, which may be because after the pathogenicity of GyH1 attracted widespread attention, breeders stepped up preventive measures to reduce the infection rate [ 14 ]. Furthermore, the molecular epidemiological and seroepidemiological results further confirm our conclusions [ 18 , 20 ].…”

The Synergy of Chicken Anemia Virus and Gyrovirus Homsa 1 in Chickens

“…GyH1 infection in chickens causes aplastic anemia, immunosuppression, and TVP [ 17 ]. Sero-epidemiological investigation indicates that GyH1 infection is widespread in chicken farms in China [ 18 ].…”

Elevation of serum human epididymis protein 4 (HE4) and N-terminal pro-B-type natriuretic peptide (NT-proBNP) as predicting factors for the occurrence of acute kidney injury on chronic kidney disease: a single-center retrospective self-control study

“…N-terminal pro-B-type natriuretic peptide (NT-proBNP), a member of the diuretic natriuretic peptide family secreted by the heart, regulates the homeostasis of blood pressure and blood volume and has a diuretic effect. NT-proBNP correlates with the occurrence of AKI and may be of great value as a biomarker for the early prediction of AKI ( Zhang et al, 2022 ). HE4, a novel tumor marker, has good predictive value for the progression of ovarian cancer ( DeBoer et al, 2018 ).…”