Serological Reactivity of Sera from Scrub Typhus Patients against Weil‐Felix Test Antigens

Amano, Ken‐ichi; Suzuki, Norihiro; Fujita, Miki; Nakamura, Yoko; Suto, Tsunehisa

doi:10.1111/j.1348-0421.1993.tb01726.x

Cited by 30 publications

(18 citation statements)

References 18 publications

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“…tsutsugamushi serotypes Karp, Kato, and Gilliam [29]. In addition, we also performed IFA for Rickettsia typhi , Rickettsia felis , Rickettsia conorii , and Coxiella burnetii .…”

Section: Methodsmentioning

confidence: 99%

Use of eschar swabbing for the molecular diagnosis and genotyping of Orientia tsutsugamushi causing scrub typhus in Quang Nam province, Vietnam

et al. 2017

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BackgroundScrub typhus is a rickettsiosis which is caused by Orientia tsutsugamushi and occurs throughout the Asia-Pacific region. Molecular diagnosis of rickettsioses using eschar swabs has recently emerged, and may be very useful for the diagnosis of these diseases in tropical settings.Methodology/Principal findingsQuantitative polymerase chain reaction (qPCR) was used to detect O. tsutsugamushi DNA in whole blood and eschar swab specimens of 67 patients who were clinically suspected of scrub typhus in Quang Nam province, Vietnam. Among the 20 patients for whom both eschar and whole blood were obtained, 17 (85%) of the eschar specimens and 5 (25%) of the whole blood specimens tested positive for O. tsutsugamushi. Genetic analysis of the 56-kDa TSA gene sequences demonstrated that the 14 sequences obtained in this study, including 12 eschar swabs and 2 whole blood specimens, were related to 4 groups: Karp, Kawasaki, Gilliam (JG-v and TG-v) and TA716. The majority (9/14; 64.4%) of contemporary O. tsutsugamushi genotypes in Quang Nam province were related to the Karp group.ConclusionsThese results suggest that polyclonal antigen pools used for serological testing in the future should contain at least Karp, Kawasaki, Gilliam and TA716 antigens for Vietnamese patients, as well as patients who have traveled to Vietnam. qPCR after eschar swabbing should be considered for molecular diagnosis of scrub typhus in endemic patients as well as in travelers, since it is easy to perform and appears very useful for the rapid detection of Orientia tsutsugamushi in the early phase of infection.

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“…tsutsugamushi serotypes Karp, Kato, and Gilliam [29]. In addition, we also performed IFA for Rickettsia typhi , Rickettsia felis , Rickettsia conorii , and Coxiella burnetii .…”

Section: Methodsmentioning

confidence: 99%

Use of eschar swabbing for the molecular diagnosis and genotyping of Orientia tsutsugamushi causing scrub typhus in Quang Nam province, Vietnam

et al. 2017

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“…Specific microimunofluorescence (IFA) assays were performed in Marseille, France, by using whole-cell antigens of O. tsutsugamushi serotypes Karp, Kato, Gilliam, and Kawasaki ( 12 ) and with Bartonella henselae , Coxiella burnetii , R. conorii subsp. indica , R. felis , R. heilongjiangensis , R. helvetica , R. honei , R. japonica , Rickettsia "ATI," R. slovaca , and R. typhi ( 13 – 15 ).…”

Section: Methodsmentioning

confidence: 99%

Rickettsial Infections and Fever, Vientiane, Laos

Phongmany

Rolain

Phetsouvanh

et al. 2006

Emerg. Infect. Dis.

204

186

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“…18 The WF test was the first technique developed, 19 but it is neither sensitive nor specific and now, rarely used. 20 The IFA test 16 and the IIP 21 test are now considered the assays of choice.…”

Section: Discussionmentioning

confidence: 99%

Dot-ELISA Rapid Test Using Recombinant 56-kDa Protein Antigens for Serodiagnosis of Scrub Typhus

Rodkvamtook¹,

Zhang²,

Chao³

et al. 2015

The American Society of Tropical Medicine and Hygiene

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Abstract. We developed a rapid dot-enzyme-linked immunosorbent assay (dot-ELISA) using the combination of recombinant 56-kDa protein antigens that exhibited broad reactivity with serum antibodies against the four most prevalent strains (Karp, Kato, Gilliam, and TA763) of Orientia tsutsugamushi. The assay is rapid (30 minutes), and can be done at room temperature, and results can be read by the naked eye. Only a simple shaker is required to wash the membrane. Sera from 338 patients suspected of being ill with scrub typhus from rural hospitals around Thailand were tested using this dot-ELISA. Seventy-five (22.2%) patients were found to be positive. The sensitivity and specificity of dot-ELISA were determined using the indirect immunofluorescent assay (IFA) test as the gold standard, with the cutoff titer of immunoglobulin peroxidase conjugate M (IgM)/G (IgG) greater than 1:400/1:400. The dot-ELISA had a sensitivity of 98.5%, a specificity of 96.3%, a positive predictive value of 86.7%, and a negative predictive value of 99.6% for the acutephase specimens. The results indicate that dot-ELISA rapid test using recombinant 56-kDa protein antigen was comparable with the IFA test and may be very useful for the diagnosis of scrub typhus in rural hospitals, where IFA is not available.

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Serological Reactivity of Sera from Scrub Typhus Patients against Weil‐Felix Test Antigens

Cited by 30 publications

References 18 publications

Use of eschar swabbing for the molecular diagnosis and genotyping of Orientia tsutsugamushi causing scrub typhus in Quang Nam province, Vietnam

Use of eschar swabbing for the molecular diagnosis and genotyping of Orientia tsutsugamushi causing scrub typhus in Quang Nam province, Vietnam

Rickettsial Infections and Fever, Vientiane, Laos

Dot-ELISA Rapid Test Using Recombinant 56-kDa Protein Antigens for Serodiagnosis of Scrub Typhus

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