Serological relationships between two microsporidian parasites of fish

Leiro, J.; Estévez, Juan José Ibáñez; Ubeira, F. M.; Santamariña, Marta; Sanmartín, M. L.

doi:10.1016/0044-8486(94)90276-3

Cited by 10 publications

(3 citation statements)

References 26 publications

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“…Xenomas were not found in intestine, liver, muscle, or stomach. Xenomas in donor fish ( (Lom 1969, Awakura 1974, McVicar 1975, Leiro et al 1994. Water-borne infections can result from direct immersion in a spore solution (Awakura 1974(Awakura , T'sui et al 1988, or by feeding a crustacean, previously exposed to spores, to fish (Awakura 1974, Olson 1976, 1981.…”

Section: Resultsmentioning

confidence: 99%

Modes of transmission of Loma salmonae (Microsporidia)

Shaw¹,

Kent²,

Adamson³

1998

Dis. Aquat. Org.

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Loma salmonae (Putz, Hoffman and Dunbar, 1965) Morrison and Sprague, 1981 (Microsporidia) causes prominent gill disease in pen-reared chinook salmon Oncorhynchus tshawytscha in the Pacific Northwest. Transmission of the parasite was examined by exposing Pacific salmon Oncorhynchus spp. to infectious spores by various routes: per OS, intraperitoneal, intramuscular, and intravascular injection, by cohabitation with infected fish, and by placement of spores directly on the gill. All exposure methods led to infections except placement of spores on the gill. Putative sporoplasms were visible in epithelial cells of the alimentary canal within 24 h of p e r os exposure. L. salmonae may initially infect alimentary epithelia1 cells and then migrate into the lamina propria to access the blood stream. Positive results obtained by intravascular injection suggest that autoinfec.tion from spores of ruptured xeno~nas in the endothelium may also occur. The cohab~tation cxpc!r~ment demonstr~ttes that flsh may become infected by spores released from live fish.

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Section: Resultsmentioning

confidence: 99%

Modes of transmission of Loma salmonae (Microsporidia)

Shaw¹,

Kent²,

Adamson³

1998

Dis. Aquat. Org.

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“…Gurley (Leiro et al . ) was detected. In any case, cross‐reactive antigens are potential targets for multifunctional vaccines against various pathogens.…”

Section: Discussionmentioning

confidence: 93%

Immunohistochemical characterization of polyclonal antibodies against Enteromyxum leei and Enteromyxum scophthalmi (Myxozoa: Myxosporea), intestinal parasites of fish

Estensoro

Redondo

Álvarez-Pellitero

et al. 2013

Journal of Fish Diseases

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The enteric myxozoan parasites Enteromyxum leei (Diamant, Lom et Dyková) and Enteromyxum scophthalmi Palenzuela, Redondo et Álvarez-Pellitero are responsible for high weight loss in infected fish, which leads to subchronic disease and low mortality rates in gilthead sea bream (GSB), Sparus aurata L., and to high mortality rates in turbot, Psetta maxima (L.). The detection of initial parasite stages in histological sections is particularly difficult, but can be simplified by means of specific antibodies. Rabbit polyclonal antibodies (pAbs) were raised against E. scophthalmi and E. leei, and direct enzyme-linked immunosorbent assay (ELISA) and immunohistochemistry were used to characterize their sensitivity and specificity. Both pAbs were adsorbed (apAb) with non-infected intestines to avoid non-specific labelling of fish tissues and to improve their specificity. The highest titre obtained in ELISA was 1: 32 000 for apAb-Eleei and 1:16 000 for apAb-Escoph. Working dilutions in immunohistochemistry were 1:1000 for apAb-Eleei and 1:8000 for apAb-Escoph. Both apAbs labelled proliferative and sporogonic stages with high specificity. apAb-Escoph was very specific, whereas apAb-Eleei cross-reacted with Sphaerospora dicentrarchi Sitjà-Bobadilla et Álvarez-Pellitero and Sphaerospora testicularis Sitjà-Bobadilla et Álvarez-Pellitero, suggesting the presence of shared antigens. These pAbs stand as new tools for antigenic characterization and the diagnosis of both Enteromyxum species.

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“…The lower antibody response in turbot may also be explained by differences in type of ELISA test used and the reagents necessary to determine the levels of antibody and/or by the fact that their adaptive immune response is less developed than that of higher vertebrates [31,32]. Differences between the immune responses of mice and fish have also been reported for other antigen preparations of parasites [33].…”

Section: Antiseramentioning

confidence: 95%