Serological responses of equids fed <i>Toxoplasma gondii</i> oocysts

Dubey, J. P.; Desmonts, G

doi:10.1111/j.2042-3306.1987.tb01426.x

Cited by 716 publications

(312 citation statements)

References 2 publications

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“…Tissue imprints of the lungs and brains of the inoculated mice that died were examined for T. gondii tachyzoites or tissue cysts. The survivors were bled on day 45 post-infection, and a 1:25 dilution of the serum was tested for T. gondii antibodies by means of the modifi ed agglutination test (MAT), as described by Dubey and Desmonts (11) . The mice were sacrifi ced on day 60 post-infection, and the brains of all the mice were examined for tissue cysts.…”

Section: Mouse Bioassaymentioning

confidence: 99%

Epidemiological aspects of Toxoplasma gondii infection in riverside communities in the Southern Brazilian Amazon

Vitaliano

Mendonça

Sandres

et al. 2015

Rev. Soc. Bras. Med. Trop.

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Introduction:Toxoplasma gondii infection is widely prevalent in humans and other animals worldwide. Information on the prevalence of T. gondii infection is scarce in some regions of Brazil, including riverside communities along the Amazon River basin. Methods: The prevalence of T. gondii in 231 people, aged 1-85 years, who were living in four riverside communities along the Purus River, Lábrea, State of Amazonas, Brazil, was determined. Antibodies against T. gondii were assayed using a commercial enzyme-linked immunosorbent assay (ELISA) kit. The hearts and brains of 50 chickens, which were raised freerange in the communities, were pooled according to the community of origin and bioassayed in mice. The isolates were genotyped using polymorphisms at 12 nuclear markers (SAG1, 5' and 3'-SAG2, alt.SAG2, SAG3, BTUB, GRA6, c22-8, c29-2, L358, PK1, Apico and CS3). Results: The overall seroprevalence of T. gondii was 56.7% (131/231). IgG antibodies were presented by 117 (89.3%) and IgM by 14 (10.7%) of the 131 positive individuals. No association between age group and gender with prevalence was observed (chi-square test, p > 0.05); however, the comparison between localities showed that the seroprevalence of T. gondii was signifi cantly lower among the individuals living in the Boca do Ituxi (p < 0.05) community. Five isolates of T. gondii were obtained in the mouse bioassay, and genotyping revealed two complete genotypes that had not been described previously and three mixed isolates. Conclusions: These results support previous fi ndings that T. gondii population genetics are highly diverse in Brazil and that T. gondii infection is active in these riverside communities.

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Section: Mouse Bioassaymentioning

confidence: 99%

Epidemiological aspects of Toxoplasma gondii infection in riverside communities in the Southern Brazilian Amazon

Vitaliano

Mendonça

Sandres

et al. 2015

Rev. Soc. Bras. Med. Trop.

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“…Initially, sera were screened at 1:16 dilution and then end titrated using a 2-fold serial dilution. Later, chicken sera were also tested at a 1:25 dilution for antibodies to T. gondii using the MAT as described previously by Dubey and Desmonts (1987). The antigen was prepared at the Laboratory of Parasitology, National Reference Centre on Toxoplasmosis (Reims, France), as described (Dubey et al, 2011) and mailed by air to Universidade de São Paulo, Brazil, from the Animal Parasitic Diseases Laboratory, USA.…”

Section: Serologic Examination Of Chickensmentioning

confidence: 99%

Seroprevalence and isolation of Toxoplasma gondii from free-range chickens from Espírito Santo state, southeastern Brazil

Beltrame

Pena

Ton

et al. 2012

Veterinary Parasitology

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“…All mice were euthanized 74 days after inoculation by cervical dislocation with prior sedation for blood collection and detection of Toxoplasma gondii. The serum was examined by the modiϐied agglutination test (MAT) (Dubey & Desmonts 1987). All mice used in the bioassay were examined for T. gondii in the tissues, as previously described (Dubey & Beattie 1988).…”

Section: Methodsmentioning

confidence: 99%

“…The carcasses were left with the owners for incineration. Serum samples were examined by modiϐied agglutination test (MAT) (Dubey & Desmonts 1987). The antigen was kindly provided by Dr. J.P. Dubey Laboratory of Parasitic Diseases of Animals, Department of Agriculture of the United States.…”

Section: Methodsmentioning

confidence: 99%

Serologic and molecular diagnostic and bioassay in mice for detection of Toxoplasma gondii in free ranges chickens from Pantanal of Mato Grosso do Sul

et al. 2012

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The aim of this study was to investigate the occurrence of Toxoplasma gondii and compare the results obtained in the Modiϐied Agglutination Test (MAT), Polimerase Chain Reaction (PCR) and bioassay in mice. In order to accomplish this, 40 free-range chickens from eight farms in neighboring areas to the Pantanal in Nhecolândia, Mato Grosso do Sul, were euthanized and blood samples, brain and heart were collected. The occurrence of anti-T. gondii antibodies found in chickens was 67.5% (27 samples), considering as a cutoff point the dilution 1:5. Among the samples analyzed, 7 (25.9%) were positive in the dilution 1:5, 3 (11.1%) in 1:10, 2 (7.4%) in 1:20, 3 (11.1%) in 1:320, 1 ( 3.7%) in 1:640, 3 (11.1%) in 1:1280, 2 (7.4%) in 1:2560, 4 (14.8%) in 1:5120 and 2 (7.4%) in 1:10.240. From the mixture of tissue samples (brain and heart) from the chickens analyzed, 16 (40%) presented electrophoretic bands compatible with T. gondii by PCR (gene B1). In the comparison of techniques, 59.26% positivity in PCR was revealed among animals that were seropositive in MAT (cutoff 1:5). From 141 inoculated mice, six (4.44%) died of acute toxoplasmosis between 15 and 23 days after inoculation. Surviving mice were sacriϐiced at 74 days after inoculation, and a total of 28 cysts were found in the brains of 10 distinct groups. From the seropositive hens, 27 bioassays were performed and 11 (40.7%) isolates were obtained. A greater number of isolations happened in mice that were inoculated with tissues from chickens that had high titers for anti-T. gondii antibodies. Chronic infection in mice was observed in nine groups (33.3%) from ϐive different properties. Among the surviving mice, 25.6% were positive for T. gondii in MAT (1:25). From mice positive in PCR, 87.5% were also positive in MAT. Among the PCR-negative mice, 5.2% were positive for T. gondii in MAT. It can be concluded through this study that the occurrence of infecton by T. gondii in the rural properties studied was high, that PCR directed to gene B1 does not conϐirm the viability of the parasite, but it can be used as a screening method for the selection of chickens infected by T. gondii, that the animals with titer greater than 10 must be prioritized for the selection of animals for bioassay, since for them, the chances of isolating the parasite are greater and that seroconversion in experimentally infected mice is not a good indicator for isolating the agent.

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Serological responses of equids fed Toxoplasma gondii oocysts

Cited by 716 publications

References 2 publications

Epidemiological aspects of Toxoplasma gondii infection in riverside communities in the Southern Brazilian Amazon

Epidemiological aspects of Toxoplasma gondii infection in riverside communities in the Southern Brazilian Amazon

Seroprevalence and isolation of Toxoplasma gondii from free-range chickens from Espírito Santo state, southeastern Brazil

Serologic and molecular diagnostic and bioassay in mice for detection of Toxoplasma gondii in free ranges chickens from Pantanal of Mato Grosso do Sul

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