Serological screening and genetic analysis of<scp>RhCE</scp>variants in the Chinese Southern Han donors

Jia, Shuangshuang; Chen, Jingwang; Wen, Jinxiu; Wang, Zhen; Wei, Ling; Fu, Yongshui; Luo, Guopei; Ji, Yanli

doi:10.1111/tme.12788

Cited by 2 publications

(2 citation statements)

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“…Their frequencies vary significantly by race and ethnicity ( 13 ). African descendants and individuals of mixed ancestry are more likely to carry RHCE variants than Caucasians and Asians ( 15 – 27 ). Using both in-house and commercial genotyping assays, researchers have investigated the diversity and frequency of RH alleles in blood samples and/or subjects with sickle cell disease at high risk of alloimmunization ( 15 – 28 ).…”

Section: Discussionmentioning

confidence: 99%

Genotype analysis to clarify RhD variants in discrepant samples of Chilean population

Aburto,

Zapata,

Retamales

et al. 2023

Front. Immunol.

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IntroductionThe D antigen variants are classified as weak, partial, and extremely weak (DEL) and can be differentiated using molecular tests. In Chile, the laboratories of local blood centers do not identify variants of the D antigen, referring them for study to the Reference Laboratory of the Public Health Institute of Chile. So, our aim was to talk about the results of the molecular analysis of variants of the D antigen in samples that had different results in the serological classification.MethodsIn the D antigen classification of the Rh system, 479 samples with serological discrepant results were sent for molecular analysis. The Rh phenotype was performed with monoclonal anti-C, anti-c, anti-E, and anti-e antisera by direct agglutination. To find the D antigen, researchers used direct agglutination with monoclonal antisera and indirect antiglobulin testing with the column (gel) agglutination method. Molecular analysis was performed with a polymerase chain reaction with sequence-specific primers (SSP-PCR) and sequencing.Results and discussionThe presence of D antigen variants was confirmed in 332 samples (69.3%), with an initial discrepancy in serological classification. In this group of discrepant samples, the frequency of weak RhD variants was 66% (219/332), that of extremely weak RhD was 28% (93/332), and that of partial RhD was 6% (20/332). The weak variants type 2 (27.4%), type 3 (8.4%), type 48 (8.4%), and type 1 (8.1%) were the next most prevalent variants after RHD*DEL43 (28%). The ccEe (R2r) phenotype was the most frequently detected (38.4%) and is present in 87% of the RHD*DEL43 samples. The E antigen is associated with the presence of this variant. Our analyses give the first description of D antigen variants in Chile. The most common variants are DEL type (RHD*DEL43) and weak (weak type 2), which are linked to the ccDEe (R2r) phenotype. These findings allow us to characterize the variants of the D antigen in Chile and, according to the obtained data, to design strategies for the management of donors, patients, and pregnant women.

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Section: Discussionmentioning

confidence: 99%

Genotype analysis to clarify RhD variants in discrepant samples of Chilean population

Aburto,

Zapata,

Retamales

et al. 2023

Front. Immunol.

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“…The uncommon DCE haplotype is found in individuals with the DCcEe or DCcEE phenotype. The number of available RhC antigen sites of CDE/cDE haplotypes was estimated as 8500–9800 per red cell, compared to 45,700–56,400 for the common CDe/Cde haplotypes [ 1 , 20 ]. In addition, the expression of RHCE*CE coding region in K562 cells showed normal expression of RhE antigen, but negative expression of RhC antigen, when tested with different anti-C MoAbs (monoclonal antibodies).…”

Section: Discussionmentioning

confidence: 99%

Five-Years Review of RHCE Alleles Detected after Weak and/or Discrepant C Results in Southern France

Pedini

Filosa

Bichel

et al. 2022

Genes

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Immunohematology laboratories are regularly facing transfusion issues due to serological weaknesses. Altered (partial) RH antigens account for most of them. In some situations, RHCE variant alleles are involved. Herein we present our three-step molecular exploration, with allele frequencies, that has efficiently untangled RH2 phenotype weaknesses and discrepancies in our 2017–2021 cohort. In the last 5 years, the PACA Corse EFS molecular platform received 265 samples from healthy blood donors or patients with C and C/e typing difficulties. The first-intention technique (DNA array and real time PCR for RHCE*CeRN research) detected RHCE variant alleles in 143 cases (54%). The RHCE alleles classically found in African populations were the most frequent, with RHCE*CeRN allele in 40 cases (15%) and (C)ces haplotype type 1 and 2 in 26 cases (10%). A “CE” effect haplotype was suspected in 56 cases, due to the uncommon DCE haplotype that may explain the low C expression. When there were no RHCE*Ce or RHCE*CE alleles, we then searched for RHD polymorphisms by DNA array. We detected the RHD*DAU5 and RHD*DIVa in 18 and 7 cases respectively, suggesting that C ambiguity is related to the presence of these alleles which has never been described with DAU5. If no variant RHCE and RHD alleles were detected, we finally sequenced the 10 exons of both RHCE and RHD genes according to the clinical context and found seven new RHCE alleles. Thus, this molecular strategy would improve the knowledge of RHCE variants’ expression and, thus, optimize the transfusion management.

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Serological screening and genetic analysis ofRhCEvariants in the Chinese Southern Han donors

Cited by 2 publications

References 35 publications

Genotype analysis to clarify RhD variants in discrepant samples of Chilean population

Genotype analysis to clarify RhD variants in discrepant samples of Chilean population

Five-Years Review of RHCE Alleles Detected after Weak and/or Discrepant C Results in Southern France

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