Serological Specificities of Murine Hybridoma Monoclonal Antibodies against<i>Neisseria meningitidis</i>Serogroups B, C, Y, and W135 and Evaluation of Their Usefulness as Serogrouping Reagents by Indirect Whole-Cell Enzyme-Linked Immunosorbent Assay

Tsang, Raymond S. W.; Zollinger, Wendell D.

doi:10.1128/cdli.12.1.152-156.2005

Cited by 12 publications

(14 citation statements)

References 32 publications

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“…Serogroup determination by standard bacterial agglutination test (24) was performed with rabbit antisera against all 13 different serogroups (A, B, C, D, H, I, K, L, W135, X, Y, Z, and 29E). Isolates that were autoagglutinable or nongroupable were tested by an indirect whole-cell enzymelinked immunosorbent assay (ELISA) using specific monoclonal antibodies to the common serogroups of B, C, Y, and W135 (33). Serotyping and serosubtyping of meningococci were done by an indirect whole-cell ELISA (1) using monoclonal antibodies against the serotype antigens 1, 2a, 2b, 4, 14, and 15 and serosubtype antigens P1.1, P1.2, P1.4, P1.5, P1.6, P1.7, P1.9, P1.10, P1.12, P1.13, P1.14, P1.15, and P1.16 (Rijksinstituut voor Volksgezondheid en Milieu, National Institute of Public Health, Bilthoven, The Netherlands).…”

Section: Methodsmentioning

confidence: 99%

Genetic and Antigenic Analysis of Invasive Serogroup Y Neisseria meningitidis Isolates Collected from 1999 to 2003 in Canada

Tsang¹,

Henderson²,

Cameron³

et al. 2007

J Clin Microbiol

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One hundred forty serogroup Y Neisseria meningitidis isolates recovered from patients with invasive meningococcal disease (IMD) in Canada from 1999 to 2003 were analyzed by genetic and serological methods. Seventy-four isolates (52.9%) belonged to serotype 2c, and most have serosubtype antigen P1.5,2 (37 isolates, 26%) or P1.5 (31 isolates, 22%). Forty-eight isolates (34.3%) belonged to serotype 14 and have serosubtype antigen P1.5,2 (13 isolates, 9%) or P1.5 (7 isolates, 5%) or were nonserosubtypeable (27 isolates, 19%). Thirteen isolates (9.3%) were nonserotypeable. Multilocus sequence typing identified two unrelated clonal populations of serogroup Y meningococci causing invasive disease in Canada: ST-23 and ST-167 clonal complexes. Almost all ST-167-related isolates were typed as 2c:P1.5, while strains of the ST-23 clonal complex were either serotype 14 or 2c but with the serosubtype antigen P1.5,2. In contrast to previous reports that patients with serogroup Y disease are usually older, 26% of the Canadian serogroup Y cases were found in the 10-to-19-year-old age group and another 11% were in the 20-to-39-year-old age group.

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Section: Methodsmentioning

confidence: 99%

Genetic and Antigenic Analysis of Invasive Serogroup Y Neisseria meningitidis Isolates Collected from 1999 to 2003 in Canada

Tsang¹,

Henderson²,

Cameron³

et al. 2007

J Clin Microbiol

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“…In addition, antibodies with binding site specificity for a small OS may functionally discriminate between simple haptens consisting of those OSs and a PS of multiple RU of the same OS (29). High-affinity immune recognition of the bacterial CPS, rather than smaller OS fragments, would lead to activation of immune effector mechanisms (31).…”

Section: Discussionmentioning

confidence: 99%

“…In the absence of immunological properties that might direct the immune response away from recognition of the sialic acid residues in each polysaccharide, it might be anticipated that sera induced by the two antigens would include populations of antibody that are highly cross-reactive and another population directed toward the surface containing C-4 of the hexose residue that would be highly specific. However, in reality in the case of the cross-reacting specificities, this is not significant, as serogrouping antisera produced in rabbits are typically used without cross-adsorption with related serogroups of meningococci (29).…”

Section: Structures Of Gymps Andmentioning

confidence: 99%

Epitope Specificities of the Group Y and W-135 Polysaccharides ofNeisseria meningitidis

Moore

Uitz

Ling

et al. 2007

Clin Vaccine Immunol

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Previous studies have identified the length dependency of several polysaccharide (PS) protective epitopes. We have investigated whether meningococcal polysaccharides Y and W-135 possess such epitopes. Oligosaccharides (OSs) consisting of one or more disaccharide repeating units (RU) were derived from the capsular PSs of group Y and W-135 meningococci (GYMP and GWMP, respectively) by mild acid hydrolysis. The relative affinities of anticapsular antibodies binding to derivative OSs of different chain lengths were measured in inhibition enzyme-linked immunosorbent assays. As OS size increased from two to three RU, there was a notable increase in binding inhibition of rabbit anti-group Y antiserum. This pattern of antibody binding inhibition was also observed for rabbit antiserum to group W-135, though the inhibition increase was much more pronounced. In the cases of both OS species, the concentration of inhibiting antigen required to achieve 50% inhibition of rabbit immunoglobulin binding increased progressively as the inhibiting disaccharide chain length increased from 1 RU through greater than 50 RU. These data suggest that antibodies directed against both of these meningococcal PSs recognize conformational epitopes only fully expressed in higher-molecularweight forms of these antigens.

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“…Although various methods, such as latex agglutination (25), coagglutination (32), the antiserum agar method (3), countercurrent immunoelectrophoresis (20), and monoclonal antibodies with enzyme-linked immunosorbent assays (ELISAs) (37), have been proposed to improve the determination of the serotype and serogroup antigens, none has gained wide acceptance, possibly because either reliable reagents are not available commercially or the procedure is tedious or requires large volumes of antisera.…”

Section: Discussionmentioning

confidence: 99%

International Circumpolar Surveillance Interlaboratory Quality Control Program for Serotyping Haemophilus influenzae and Serogrouping Neisseria meningitidis, 2005 to 2009

et al. 2012

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Discrepancies were observed most frequently for serogroups W135, X, Z, and 29E. The overall serotype concordance for H. influenzae was 98% (125/127 attempts). The two discrepant results involved a serotype c strain and a serotype e strain, and in both cases, the serotypeable H. influenzae isolates were misidentified as being nontypeable. These data demonstrate a high degree of concordance for serogroup and serotype determinations of N. meningitidis and H. influenzae isolates, respectively, among the five laboratories participating in this quality control program.

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Serological Specificities of Murine Hybridoma Monoclonal Antibodies againstNeisseria meningitidisSerogroups B, C, Y, and W135 and Evaluation of Their Usefulness as Serogrouping Reagents by Indirect Whole-Cell Enzyme-Linked Immunosorbent Assay

Cited by 12 publications

References 32 publications

Genetic and Antigenic Analysis of Invasive Serogroup Y Neisseria meningitidis Isolates Collected from 1999 to 2003 in Canada

Genetic and Antigenic Analysis of Invasive Serogroup Y Neisseria meningitidis Isolates Collected from 1999 to 2003 in Canada

Epitope Specificities of the Group Y and W-135 Polysaccharides ofNeisseria meningitidis

International Circumpolar Surveillance Interlaboratory Quality Control Program for Serotyping Haemophilus influenzae and Serogrouping Neisseria meningitidis, 2005 to 2009

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