Serological studies in patients on platelet‐ and granulocyte‐substitution therapy

Pegels, J. G.; Bruynes, E.C.E.; Engelriet, C.P.; Borne, A. E. G. Kr.

doi:10.1111/j.1365-2141.1982.tb03861.x

Cited by 76 publications

(30 citation statements)

References 25 publications

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“…The finding of a low frequency (less than 1%) of plateletspecific antibodies after one series of blood transfusions is in accordance with the results of some previous studies in multiply transfused patients [5][6][7][8] but not with those of oth er studies [1][2][3][4] in which the incidence of platelet antibodies was estimated to be in the range of20-50%.…”

Section: Discussionsupporting

confidence: 80%

“…The frequency of alloantibodies directed against platelet-specific antigens in multiply transfused patients is controversial. Several authors consider that they are fre quent with an incidence in the range of 15-50% [1][2][3][4]. In contrast, other authors using specific assays have reported that platelet-specific antibodies occur in about 1-5% of pa Patients and Materials 2 Preoperative blood samples were investigated for HLA antibodies, platelet-specific antibodies and platelet counts.…”

Section: Introductionmentioning

confidence: 99%

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Platelet Alloimmunization after Transfusion

Taaning¹,

Simonsen²,

Hjelms³

et al. 1997

Vox Sanguinis

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Background and objectives: The frequency of platelet-specific antibodies after one series of blood transfusions has not been reported, and in multiply transfused patients is controversial. Materials and methods: We studied the frequency of alloimmunization against platelet antigens in 117 patients who received a single series of blood transfusions. They received mostly saline-adenine-glucose + mannitol red blood cell components (poor in leukocytes and platelets) in connection with cardiac surgery. Platelet-specific antibodies were detected with the platelet ELISA and the monoclonal-antibody-specific immobilization of platelet antigen assay. HLA antibodies were detected by the standard lymphocyte cytotoxicity techniques. Results: We found platelet-specific anti-HPA-5b (anti-Bra) in 2 cases (1.7%). One antibody was the result of de novo immunization. We detected lymphocytotoxic HLA antibodies in 21 patients (17.9%), of whom 18 (15.4%) had had no detectable antibodies before transfusion. There was a positive correlation between the transfused load of immunogenic materials and the frequency of alloimmunization against HLA antigens. In one third of the immunized patients, there was no history of previous immunization. Conclusion: There was a low incidence of platelet-specific antibodies after one series of blood transfusions in this group of patients. This is similar to the results of some previous studies in multiply transfused patients, but not with those of others who found a higher incidence.

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Section: Discussionsupporting

confidence: 80%

Section: Introductionmentioning

confidence: 99%

Platelet Alloimmunization after Transfusion

Taaning¹,

Simonsen²,

Hjelms³

et al. 1997

Vox Sanguinis

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“…Immunization may develop after a min imal number of platelet transfusions [1,2], but some patients remain responsive to random platelets for years having received hundreds of donor units [3,4]. After the introduction of platelet transfusions the reported inci dence of immunization varies between 35 and 100% [2][3][4][5][6][7][8][9][10],…”

Section: Introductionmentioning

confidence: 99%

Alloimmunization after Leukocyte-Depleted Multiple Random Donor Platelet Transfusions

et al. 1988

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In a prospective study we investigated the development and the course of alloimmunization after leukocyte-depleted red cell and multiple random donor platelet transfusions in 335 patients. Of these 335 patients, who had a negative antibody screening on admission and a negative transfusion history, 69 (21%) developed either transient (n= 18) or permanent (n = 51 ) lymphocytotoxic antibodies, but only 31 patients (9%; 95% confidence limits 6-12%) developed multispecific alloantibodies necessitating HLA-matched platelet transfusions. There was no difference with regard to the development of antibodies and platelet refractoriness between leukemia patients receiving cytostatic treatment and patients with aplastic anemia receiving prednisone and antithymocyte globulin. Females with previous pregnancies developed platelet refractoriness with an increased incidence (χ^2 13.38; p<0.001) compared to females without previous pregnancies, males, and children.

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“…Immune complexes are common in patients with fever and may increase the platelet turnover. It was the contribution of Pegels et al [13], who demonstrated that patients with septicemia may produce de novo platelet-specific antibodies. How does fever trigger the production of platelet antibodies?…”

Section: Discussionmentioning

confidence: 99%

Platelet Antibodies and Fever: Their Association in Multitransfused Patients with Hemato-Oncological Diseases

Kurz¹,

Eichelberger²,

Greinix³

et al. 1999

Transfus Med Hemother

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The posttransfusion platelet increment depends significantly on the clinical condition of the patient and the presence of platelet-reactive antibodies. We prospectively determined the development of platelet-reactive antibodies in weekly intervals at the time of chemotherapy in 17 multitransfused patients with hemato-oncological diseases. Sera were tested by both the MAIPA technique and the lymphocytotoxicity test (LCT) for antiplatelets against HLA class I antigens. In addition, we applied the MAIPA technique to determine the presence of platelet-reactive antibodies against human platelet-specific alloantigens (HPA-1, -2, -3, -5) and the platelet membrane glycoproteins (GP) Ia/IIa, Ib/IX, IIb/IIIa (panreactive). Platelet-reactive HLA antibodies were the most common antibodies, found in the sera of 8 patients. They were transient in 5 patients and were constantly detectable throughout the observation period in the other 3. Ten sera from 7 patients were positive for HLA antibodies by MAIPA, but negative by LCT. Of note, in 5 patients the presence of antibodies was indicated by MAIPA, but the LCT became positive only in the consecutive sample.

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Serological studies in patients on platelet‐ and granulocyte‐substitution therapy

Cited by 76 publications

References 25 publications

Platelet Alloimmunization after Transfusion

Platelet Alloimmunization after Transfusion

Alloimmunization after Leukocyte-Depleted Multiple Random Donor Platelet Transfusions

Platelet Antibodies and Fever: Their Association in Multitransfused Patients with Hemato-Oncological Diseases

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