SEROLOGICAL STUDY OF
            <i>BORDETELLA PERTUSSIS</i>
            AND RELATED SPECIES

Eldering, Grace; Hornbeck, Chester; Baker, Julia

doi:10.1128/jb.74.2.133-136.1957

Cited by 84 publications

(40 citation statements)

References 1 publication

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“…Agglftinogen serotping. B. pertussis strains were serotyped by the microagglutination assay (19a) using typing sera prepared by G. Eldehng (11,12).…”

Section: Methodsmentioning

confidence: 99%

Filamentous hemagglutinin has a major role in mediating adherence of Bordetella pertussis to human WiDr cells

Urisu¹,

Cowell²,

Manclark³

1986

Infect Immun

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35S]methionine-labeled Bordetella pertussis adhered to monolayers of WiDr cells, an epitheliumlike cell line from a humah intestinal carcinoma. Adherence was proportional to the density of the WiDr cells and to the concentration of B. pertussis in the assay. Adherence of virulent phase I strains Tohama phase I, 114, and BP338 was much greater than adherence of avirtilent strains Tohama phase Ill and 423 phase IV. Muta,nts deficient in the production of the filamentous hemaggilitinin (FHA) were hemagglutination negative and adhered to WiDr cells much less efficiently than the parent strains. Preincubation of B. pertussis cells with FHA increased their hemagglutination activity and adherence to WiDr cells. Goat antibody to FHA inhibited, in a dbse-dependent manner, the adherence of strain Tohama I but not the adherence of FHA-deficient mutant Tohaina 325. At similar protein concentrations, normnal goat antibody, goat antibody to-pertussis toxin, or the Fab fragments of goat antibody to serotype 2 fimbriae had nb effect on adherence. Also, an FHA-positive strain withoit fimbriae showed high adherence, while a fimbriated FHA-defident mutant adhered poorly. Our data indicate that FHA plays a major role in adherence ofB. pertussis to human WilDr cells. Fimbriae do not appear to mediate attachment of B. pertussis to WiDr cells.

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“…Agglftinogen serotping. B. pertussis strains were serotyped by the microagglutination assay (19a) using typing sera prepared by G. Eldehng (11,12).…”

Section: Methodsmentioning

confidence: 99%

Filamentous hemagglutinin has a major role in mediating adherence of Bordetella pertussis to human WiDr cells

Urisu¹,

Cowell²,

Manclark³

1986

Infect Immun

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“…One explanation may be that B. pertussis serotype I .2 bacteria have more structural similarity to B. parapertussis than to B. pertussis type 1.3 bacteria. Adsorption with B. parapertussis cells is usually not included in factor serum production (4, 6,14,18). Our study illustrates potential difficulties in serotyping with polyclonal antisera rendered specific by adsorption only with heterologous B. pertussis strains.…”

Section: Temperature-sensitive Agglutination With the Agla Monoclonalmentioning

confidence: 80%

THE SPECIFICITY OF ANTISERA AGAINST BORDETELLA PERTUSSIS EXAMINED BY BACTERIAL AGGLUTINATION

Fredriksen¹,

Frøholm²,

Kjennerud³

1987

Acta Pathologica Microbiologica Scandinavica Series B: Microbio

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The specificity of conventional, adsorbed antisera against agglutinogens 1, 2, and 3 of Bordetella pertussis was examined by slide agglutination and by agglutination in microtitre wells. Unadsorbed hyperimmune sera showed higher agglutinating activity against autologous or homologous cells than against cells of heterologous serotype. Adsorption of sera with heterologous cells increased the serotype specificity considerably. In spite of extensive adsorption, these anti‐agglutinogen sera were still found to cross‐react with B. parapertussis and/or B. bronchiseptica strains. Adsorption experiments with B. pertussis hyperimmune sera against serotype 1‐, 1.2‐, and 1.3‐organisms demonstrated that the cross‐reacting surface antigens differed from the agglutinogens 1, 2, and 3. Thus, in making species‐specific reagents for diagnostic use it may be of value to include adsorption with B. parapertussis and probably with B. bronchiseptica. Limited data indicated that there is no need to use B. avium for adsorption. The agglutination assays were also used to test three monoclonal antibodies stated to be specific for the agglutinogens 1, 2, and, 3, respectively. Some anomalous behaviour for the anti‐agglutinogen 1 reagent was found, whereas the anti‐agglutinogen 2 and 3 reagents corresponded well with the present polyclonal factor sera.

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“…It is possible that this data may be of more general application in analyzing the relationship between host and parasite and the mechanism of pathogenesis and of immunological defense in bacterial infection, especially for organisms inhabiting the respiratory tract. These results may be particularly pertinent to Bordetella pertussis, which has antigenically similar hemagglutinin (8), dermonecrotic toxin, and K-agglutinogen in common with B. bronchiseptica (2,5,9,16). Accordingly, we are hoping to clarify the mechanisms which enable the complete clearance of organisms from the respiratory tract, especially from the nasal cavity.…”

Section: Results Lmentioning

confidence: 95%

Protection against experimental Bordetella bronchiseptica infection in mice by active immunization with killed vaccine

Sekiya¹,

Kawahira²,

Nakase³

1983

Infect Immun

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Mice immunized with a killed vaccine of phase I Brodetella bronchiseptica were challenged with various numbers of virulent B. bronchiseptica by intraperitoneal, intracerebral, or intranasal routes. The course of infection was compared among these routes, and the protective effect of vaccination was quantitatively analyzed. In ddN mice infected intraperitoneally with 1.8 x 108 cells (ca. 80 times the 50% lethal dose [LD50]) the organisms rapidly increased in the intraperitoneal fluid, spleen, and liver within few days and caused splenic atrophy, septicemia, and death. However, immunizations with 5 x 109 cells gave the mice a high agglutinin titer and suppressed the increase in the number of organisms. With four immunizations, the lungs and livers were clear within 3 days, and with one or two immunizations, they were clear within 7 days. These immunizations effectively protected the mice from death but did not protect them from splenic atrophy. In the intracerebral infection with 1.4 x 106 cells (ca. 1.2 x 105 LD50), the number of organisms rapidly increased in the brain and caused encephalitis, splenic atrophy, and death. However, four or five immunizations completely suppressed the increase in the brain and protected the mice from death and splenic atrophy. After intranasal infection with 4 x 106 cells (ca. 25 LD50), the organisms rapidly increased in the nasal cavity and lungs and caused pneumonia and death.

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SEROLOGICAL STUDY OF BORDETELLA PERTUSSIS AND RELATED SPECIES

Cited by 84 publications

References 1 publication

Filamentous hemagglutinin has a major role in mediating adherence of Bordetella pertussis to human WiDr cells

Filamentous hemagglutinin has a major role in mediating adherence of Bordetella pertussis to human WiDr cells

THE SPECIFICITY OF ANTISERA AGAINST BORDETELLA PERTUSSIS EXAMINED BY BACTERIAL AGGLUTINATION

Protection against experimental Bordetella bronchiseptica infection in mice by active immunization with killed vaccine

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