Serological survey of virus infection among wild house mice (<i>Mus domesticus</i>) in the UK

Becker, S.; Bennett, Malcolm J.; Stewart, James P.; Hurst, Jane L.

doi:10.1258/002367707780378203

Cited by 75 publications

(57 citation statements)

References 41 publications

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“…In total 1,147 blood samples were collected from rodents: 1,060 were live-trapped, wild animals from <20 sites (Table 1), and 87 blood samples were collected from a captive colony of wild house mice (9) and tested serologically.…”

Section: The Studymentioning

confidence: 99%

Host Range and Genetic Diversity of Arenaviruses in Rodents, United Kingdom

Blasdell¹,

Becker²,

Hurst³

et al. 2008

Emerg. Infect. Dis.

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Section: The Studymentioning

confidence: 99%

Host Range and Genetic Diversity of Arenaviruses in Rodents, United Kingdom

Blasdell¹,

Becker²,

Hurst³

et al. 2008

Emerg. Infect. Dis.

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“…MHV68 is genetically colinear to the human gammaherpesviruses and exhibits many similar pathogenic features (54,62). MHV68 is a natural pathogen of rodents (6,9,44), making the inoculation of mice with MHV68 a useful small-animal model to study gammaherpesvirus infection in vivo.…”

mentioning

confidence: 99%

Use of a Virus-Encoded Enzymatic Marker Reveals that a Stable Fraction of Memory B Cells Expresses Latency-Associated Nuclear Antigen throughout Chronic Gammaherpesvirus Infection

Nealy

Coleman

et al. 2010

J Virol

102

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An integral feature of gammaherpesvirus infections is the ability to establish lifelong latency in B cells. During latency, the viral genome is maintained as an extrachomosomal episome, with stable maintenance in dividing cells mediated by the viral proteins Epstein-Barr nuclear antigen 1 (EBNA-1) for Epstein-Barr virus and latencyassociated nuclear antigen (LANA) for Kaposi's sarcoma-associated herpesvirus. It is believed that the expression of episome maintenance proteins is turned off in the predominant long-term latency reservoir of resting memory B cells, suggesting that chronic gammaherpesvirus infection is primarily dormant. However, the kinetics of LANA/ EBNA-1 expression in individual B-cell subsets throughout a course of infection has not been examined. The infection of mice with murine gammaherpesvirus 68 (MHV68, ␥HV68) provides a model to determine the specific cellular and molecular events that occur in vivo during lifelong gammaherpesvirus latency. In work described here, we make use of a heterologously expressed enzymatic marker to define the types of B cells that express the LANA homolog (mLANA) during chronic MHV68 infection. Our data demonstrate that mLANA is expressed in a stable fraction of B cells throughout chronic infection, with a prominent peak at 28 days. The expression of mLANA was detected in naïve follicular B cells, germinal-center B cells, and memory B cells throughout infection, with germinalcenter and memory B cells accounting for more than 80% of the mLANA-expressing cells during the maintenance phase of latency. These findings suggest that the maintenance phase of latency is an active process that involves the ongoing proliferation or reseeding of latently infected memory B cells.Gammaherpesviruses such as Epstein-Barr virus (EBV), Kaposi's sarcoma-associated virus (KSHV, HHV-8), and murine gammaherpesvirus 68 (MHV68, ␥HV68) are associated with lymphoproliferative diseases and a variety of malignancies of both epithelial and lymphoid origin. The strict species specificity exhibited by gammaherpesviruses has limited research on the human viruses primarily to in vitro studies. MHV68 is genetically colinear to the human gammaherpesviruses and exhibits many similar pathogenic features (54, 62). MHV68 is a natural pathogen of rodents (6, 9, 44), making the inoculation of mice with MHV68 a useful small-animal model to study gammaherpesvirus infection in vivo.A hallmark of gammaherpesvirus infections is the establishment of lifelong latency in B cells. During latency, the viral genome is maintained as an extrachromosomal episome, viral gene expression is highly restricted, and no new progeny virions are generated. The stable maintenance of the episome in dividing cells requires regulated plasmid DNA replication and the efficient partitioning of replicated genomes to daughter cells. These processes are mediated by critical episome maintenance protein Epstein-Barr nuclear antigen 1 (EBNA-1) for EBV and latency-associated nuclear antigen (LANA) for KSHV. EBNA-1 and LANA facilitate the re...

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“…For the ELISAs using SeV-NP peptides, the dilution ratio of sera was 1:200, which was higher than that of the commercial ELISA. The antigen in the commercial ELISA was ether-treated entire SeV virions [18,19] [4]. We considered that the ELISAs using the SeV-NP peptides might be suitable for the wild house mouse, which is the same species as laboratory mice.…”

Section: Discussionmentioning

confidence: 99%

Epitope Mapping of the Nucleocapsid Protein of Sendai Virus and Application of Antigenic Epitopes for the ELISA-Based Diagnosis of Sendai Virus Infection

Asano

Torigoe

Sasaki

et al. 2013

The Journal of Veterinary Medical Science

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ABSTRACT. Sendai virus (SeV) is one of the most prevalent viral pathogens infecting laboratory mice and rats. To date, mature SeV virions have been used as antigens for serological diagnosis. To develop antigens that are more specific and easier to prepare for diagnosis, we examined the antigenic sites in the nucleocapsid protein (NP) of SeV with antisera from experimentally SeV-infected mice and a peptide array membrane containing overlapping 10-mer peptides covering the entire NP. We found antigenic linear sequences in two regions, amino acids 120-160 and 420-500, of the SeV-NP. From these antigenic sequences, we applied two synthesized peptides, IVKTRDMEYERTTEWL and FVTLHGAERLEEETNDE, which correspond to positions 119-134 and 458-474 of the SeV-NP, respectively, as antigens in an enzymelinked immunosorbent assay (ELISA). Evaluation of the ELISAs using these peptides revealed that they were specific to anti-SeV antisera. Furthermore, the ELISAs using these peptides were able to distinguish between SeV-positive and SeV-negative mouse sera to the same extent as a commercial ELISA kit. These results indicate that these peptides are useful for the serological diagnosis of SeV infection.

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Serological survey of virus infection among wild house mice (Mus domesticus) in the UK

Cited by 75 publications

References 41 publications

Host Range and Genetic Diversity of Arenaviruses in Rodents, United Kingdom

Host Range and Genetic Diversity of Arenaviruses in Rodents, United Kingdom

Use of a Virus-Encoded Enzymatic Marker Reveals that a Stable Fraction of Memory B Cells Expresses Latency-Associated Nuclear Antigen throughout Chronic Gammaherpesvirus Infection

Epitope Mapping of the Nucleocapsid Protein of Sendai Virus and Application of Antigenic Epitopes for the ELISA-Based Diagnosis of Sendai Virus Infection

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