Serology and Genetics of an MNSs‐Associated Antigen Dantu

Abstract

Dantu, a previously undescribed low-incidence red cell antigen, is inherited as a Mendelian dominant character. The Dantu antigen is associated with very weak s antigen, protease resistant N antigen and either very weak or no U antigen. Two of the propositi had previously been shown to have an unusual hybrid MNSs sialoglycoprotein, and it is probably this which carries these unusual N, s and U antigens as well as the Dantu antigen. A study of the family of one propositus suggests, by conventional genetics, that Dantu is not controlled by the MNSs locus; a possible explanation is given. Several examples of anti-Dantu are known, one was found to cause a positive direct antiglobulin reaction on neonatal red cells.

Blood Grouping With Monoclonal Anti‐n Antibodies

“…The Dantu antigen has recently been shown to be associated with an unusual protease-resistant N antigen (Contreras et al, 1984). N3 has been shown to agglutinate cells of the Dantu/MS, Dantu/Ms and Dantu/Mu types, presumably due to recognition of the "N' antigen borne by the Dantu sialoglycoprotein molecule (Moores, personal communication).…”

Hybrid glycophorins from human erythrocyte membranes. I. Isolation and complete structural analysis of the hybrid sialoglycoprotein from Dantu-positive red cells of the N. E. variety

“…Anti-Dantu belongs to the group of antibodies against low-frequency antigens that occur frequently in human sera under conditions of increased erythrocyte destruction [13]. There is evidence that the formation of some of these antibodies might be triggered by cryptic structures in normal glycoproteins that are covered by carbohydrate chains [S, 421. Since the Dantu antigen appears to be located in a carbohydrate-free region of the hybrid molecule, the hypothesis mentioned above does not provide an explanation for the frequent occurrence of anti-Dantu.…”

“…(genotype DantulMu) described previously [I, 2, 12, 131 and an additional Black donor (genotype DantulMs). Some studies were also performed on a pool of Dantu-positive erythrocytes from ten unrelated individuals detected in Chicago or Houston or by several colleagues (T. Stout, Vancouver; M. Contreras, London [13]; K. Skradsky, Minneapolis). Two human anti-S sera containing anti-Dantu and an anti-Dantu made by a pregnant woman (H.P.…”

Anti-Gerbich Antibodies and Gerbich-Negative Dantu-Positive Red Blood Cells in a Woman from South Africa

“…The membranes of the cells contain a hybrid 8-a form of SGP of which two variants, Ph and NE, are known [7]. The antigens be sides Dantu encoded by the Dantu gene complex are variable strength M and N, no or very weak U and an unusual form of In this paper, studies with the blood of a family of mixed ethnic origin from Cape Town are reported.…”

“…Dantu is a low-frequency red cell anti gen of the MNSs system found so far mainly in people of Negro origin [7]. The membranes of the cells contain a hybrid 8-a form of SGP of which two variants, Ph and NE, are known [7].…”