Serology

Abstract

Serological diagnosis is noninvasive and relatively economic and has less possibility of showing false-negative results in the situations of hemorrhagic ulcer or taking medications like antibiotics. In addition, it performs mass epidemiological survey simultaneously. However, it is not recommended for monitoring changes after antimicrobial treatment because this diagnosis hardly differentiates past Helicobacter pylori ( H. pylori ) infection and current active infection. There are various types of serological diagnoses of H. pylori existence such as bacterial agglutination, complement fi xation, indirect immunofl uorescence test (IIF), enzyme immunoassay (EIA), and enzyme-linked immunosorbent assay (ELISA). Among those, ELISA is the most popular method in the world. KeywordsHelicobacter pylori • Serology • Diagnosis • ELISA 8.1

Trends in the seroprevalence of Helicobacter pylori infection and its putative eradication rate over 18 years in Korea: A cross-sectional nationwide multicenter study

“…To reduce the false-positive rate, subjects undergoing HP eradication therapy were excluded because seroconversion is very slow after HP eradication, and the anti- HP specific IgG may remain positive even in eradicated cases [ 32 ]. However, serologic tests are widely available, noninvasive, inexpensive and appropriate for screening in large epidemiologic studies [ 12 ]. Second, the medium-to-high socioeconomic status of our study subjects might also lead to selection bias.…”

“…HPG kits (Immulite 2000 CMIA, Siemens, UK) were used at the SNUHGC, and Genedia HP ELISA kits (Green Cross Medical Science Corp., Eumsung, Korea) were used at the remaining centers [ 10 ]. The HPG kit used a chemiluminescent enzyme immunoassay and had a sensitivity of 91% and a specificity of 100% [ 12 , 13 ]. Anti- HP IgG levels higher than 1.10 IU/mL were regarded as positive in the Immulite 2000 assay, while values in the range from 0 to 1.09 IU/mL were considered negative.…”