Serology of Severe Acute Respiratory Syndrome: Implications for Surveillance and Outcome

Chen, Xinchun; Zhou, Boping; Li, Meizhong; Liang, Xiaorong; Wang, Huo-sheng; Yang, Guang; Wang, Hui; Le, Xiaohua

doi:10.1086/380397

Cited by 68 publications

(57 citation statements)

References 9 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…All patients had a history of exposure to SARS patients. During hospital admission, all patients had evidence of SARS-CoV infection detected by ELISA for anti-SARS-CoV-specific IgG Ab in the serum and/or RT-PCR for the SARS virus, as described previously (21). In the convalescent cohort study, PBMC were isolated from whole blood by Ficoll-Hypaque density gradient centrifugation for an immediate IFN- γ ELISPOT assay.…”

Section: Methodsmentioning

confidence: 99%

T Cell Responses to Whole SARS Coronavirus in Humans

et al. 2008

The Journal of Immunology

492

537

View full text Add to dashboard Cite

Section: Methodsmentioning

confidence: 99%

T Cell Responses to Whole SARS Coronavirus in Humans

et al. 2008

The Journal of Immunology

492

537

View full text Add to dashboard Cite

“…Conventional IFAs (cIFAs) using virus-infected African green monkey kidney cells (VERO E6) spotted on glass slides and ELISAs using extracts or supernatant of infected cells were among the first assays used in serological diagnosis of SARS-CoV Chen et al, 2004a;Hsueh et al, 2003;Ksiazek et al, 2003;Peiris et al, 2003;Wu et al, 2004). Both cIFAs and ELISAs for SARS-CoV were relatively easy to set up for experienced laboratories, as they primarily only required susceptible cell cultures and the virus.…”

Section: Virus-based Serological Assaysmentioning

confidence: 99%

Serological assays for emerging coronaviruses: Challenges and pitfalls

2014

View full text Add to dashboard Cite

More than a decade after the emergence of severe acute respiratory syndrome coronavirus (SARS-CoV) in 2002/2003 the occurrence of a novel CoV termed Middle East respiratory syndrome (MERS) CoV challenges researchers and public health authorities. To control spread and finally contain novel viruses, rapid identification and subsequent isolation of infected individuals and their contacts is of utmost importance. Next to methods for nucleic acid detection, validated serological assays are particularly important as the timeframe for antibody detection is less restricted. During the SARS-CoV epidemic a wide variety of serological diagnostic assays were established using multiple methods as well as different viral antigens. Even though the majority of the developed assays showed high sensitivity and specificity, numerous studies reported on cross-reactive antibodies to antigens from wide-spread common cold associated CoVs. In order to improve preparedness and responsiveness during future outbreaks of novel CoVs, information and problems regarding serological diagnosis that occurred during the SARS-CoV should be acknowledged. In this review we summarize the performance of different serological assays as well as the applicability of the two main applied antigens (spike and nucleocapsid protein) used during the SARS-CoV outbreak. We highlight challenges and potential pitfalls that occur when dealing with a novel emerging coronavirus like MERS-CoV. In addition we describe problems that might occur when animal sera are tested in serological assays for the identification of putative reservoirs. Finally, we give a recommendation for a serological testing scheme and outline necessary improvements that should be implemented for a better preparedness.

show abstract

“…Serologic testing is the most sensitive test but it takes several weeks for antibodies to develop, with a mean time to seroconversion of approximately 18 to 20 days. 106,107 Treatment of SARS is supportive, with little evidence of any effective antivirals or other pharmacologic therapies. 108 A novel coronavirus was discovered in September 2012, which has now been referred to as the Middle East respiratory syndrome (MERS) coronavirus.…”

Section: Severe Acute Respiratory Syndrome and Other Coronavirusesmentioning

confidence: 99%

Influenza and Endemic Viral Pneumonia

Ramsey

Kumar

2013

Critical Care Clinics

View full text Add to dashboard Cite

Viruses are a common and important cause of severe community-acquired pneumonia, and may lead to severe respiratory disease and admission to the intensive care unit. Influenza is the most common virus associated with severe viral pneumonia, although other important causes include respiratory syncytial virus, adenovirus, metapneumonia virus, and coronaviruses. Viral pneumonias tend to have a seasonal predilection and are often preceded by a typical viral prodrome. This article focuses on severe influenza pneumonia, including the 2009 H1N1 pandemic, and briefly discusses other causes of severe respiratory disease of viral etiology.

show abstract

Serology of Severe Acute Respiratory Syndrome: Implications for Surveillance and Outcome

Abstract: Detection of antibody to SARS CoV is useful in the diagnosis of SARS; however, at the incubation and initial phases of the illness, serological assay is of little value, because of late seroconversion in most patients.

Cited by 68 publications

References 9 publications

T Cell Responses to Whole SARS Coronavirus in Humans

T Cell Responses to Whole SARS Coronavirus in Humans

Serological assays for emerging coronaviruses: Challenges and pitfalls

Influenza and Endemic Viral Pneumonia

Contact Info

Product

Resources

About