Seronegative HIV-2 carriers in India

Kageyama, Seiji; Maniar, Janak K.; Iwasaki, Masaomi; Zhang, J; Saple, D. G.; Tsuchie, Hideaki; Tanabe-Tochikura, Akiko; Taniguchi, Keigo; Shiraki, Kimíyasu; Kurimura, Takashi

doi:10.1258/0956462001914878

Cited by 8 publications

(5 citation statements)

References 12 publications

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“…30 Peripheral blood mononuclear cells (PBMCs) may yield a better outcome with regard to HIV-2 detection. 30,31 The limitations of this study include small sample size, quality and type of the specimens used as described here.…”

Section: Discussionmentioning

confidence: 99%

HIV-1/2 differentiation in a South African public laboratory

Mafuyeka¹,

Webber²,

Becker³

et al. 2021

South. Afr. j. HIV med.

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Background: The human immunodeficiency virus type-2 (HIV-2) prevalence in South Africa (SA) is unknown, however, sporadic cases have been reported. Human immunodeficiency virus -1 and 2 differentiation is not part of most South African public laboratories’ testing algorithm. Human immunodeficiency virus -2 diagnosis using serology assays may be complicated by HIV-1 and HIV-2 antibody cross-reactivity.Objectives: To determine the proportion of HIV-2 infections in specimens that tested HIV-1/2 positive at a public laboratory in Tshwane.Method: A total of 480 specimens that were previously tested with fourth generation ELISA platforms (Modular E170 [Roche, Switzerland] and Architect i2000 [Abbott, Germany]) were randomly selected. Human immunodeficiency virus -1 and 2 antibody differentiation testing was carried out using the Multispot HIV-1/2 rapid assay (Bio-Rad Laboratories, USA). An in-house nested HIV-2 PCR assay targeting the 5′-long terminal repeats (5′-LTR) region was evaluated and used as a confirmatory test.Results: The study tested 480 HIV-1/2 seropositive patients and their mean age was 36.7 years (range 3–82 years). Of the 480 patients, 292 (60.8%) were female, 182 (37.9%) were male and 6 (1.3%) were not specified. Human immunodeficiency virus differentiation results were as follows: 466 (97.1%) were positive for only HIV-1 antibodies, 11 (2.3%) [95%CI: (0.98%; 3.74%)] were positive for both HIV-1 and HIV-2 antibodies, 3 (0.6%) were negative for both antibodies and none were positive for only HIV-2 antibodies. Of the 11 specimens with both HIV-1 and HIV-2 antibodies, seven had sufficient volume for confirmatory testing and were all negative on the in-house HIV-2 PCR assay.Conclusion: The multispot HIV-1/2 rapid assay demonstrated cross-reactivity between HIV-1 and HIV-2 antibodies. Human immunodeficiency virus -2 infections were not detected.

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Section: Discussionmentioning

confidence: 99%

HIV-1/2 differentiation in a South African public laboratory

Mafuyeka¹,

Webber²,

Becker³

et al. 2021

South. Afr. j. HIV med.

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show abstract

“…The presence of certain HLA alleles with seronegativity to disease has been reported in malaria (10), HIV (16,17), rheumatoid arthritis (RA) (18) and spondylarthropathies (SpA) (19). HLA-DR1 was associated with seronegative/weakly seropositive RA and HLA-DR4 was associated with seropositive RA.…”

Section: Discussionmentioning

confidence: 99%

Contribution of HLA alleles in the regulation of antibody production in lyme disease

Wang¹

2001

Front Biosci

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A small subset of patients infected with Borrelia burgdorferi (Bb) does not produce Bb specific antibody. Our research provides additional evidence of a genetic predisposition for seronegativity in some individuals with Lyme disease. Because human leukocyte antigen (HLA) class II, a heterodimeric glycoprotein, plays an essential role in the regulation of antibody production, we investigated the difference in HLA genes between seropositive and seronegative patients with Lyme disease (LD). Our results show that HLA-DR7 was associated with anti-Bb antibody production. Nine out of the 22 seropositive LD patients (40.9%) had HLA-DRB1*0701, *0703, *0704 (HLA-DR7); only 1 out of the 18 seronegative LD patients (5.6%) had HLA-DR7 (odds ratio (OR)=11.8, P=0.0126). HLA-DRB1*01021 and HLA-DRB1*0101, *0104, *0105 (HLA-DR1) contributed negatively to anti-Bb antibody production. Seven of 18 seronegative LD patients had HLA-DR1, only 1 of 22 seropositive LD patients had HLA-DR1 (38.9% vs. 4.5%, OR=13.4, P=0.0138). These results suggest that the presence and or lack of production of specific antibody to Bb infection may be associated with particular HLA specificities of the Class II.

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Section: Discussionmentioning

confidence: 99%

Contribution of HLA alleles in the regulation of antibody production in lyme disease

Wang¹

2001

Front Biosci

View full text Add to dashboard Cite

A small subset of patients infected with Borrelia burgdorferi (Bb) does not produce Bb specific antibody. Our research provides additional evidence of a genetic predisposition for seronegativity in some individuals with Lyme disease. Because human leukocyte antigen (HLA) class II, a heterodimeric glycoprotein, plays an essential role in the regulation of antibody production, we investigated the difference in HLA genes between seropositive and seronegative patients with Lyme disease (LD). Our results show that HLA-DR7 was associated with anti-Bb antibody production. Nine out of the 22 seropositive LD patients (40.9%) had HLA-DRB1*0701, *0703, *0704 (HLA-DR7); only 1 out of the 18 seronegative LD patients (5.6%) had HLA-DR7 (odds ratio (OR)=11.8, P=0.0126). HLA-DRB1*01021and HLA-DRB1*0101, *0104, *0105 (HLA-DR1) contributed negatively to anti-Bb antibody production. Seven of 18 seronegative LD patients had HLA-DR1, only 1of 22 seropositive LD patients had HLA-DR1 (38.9% vs. 4.5%, OR=13.4, P=0.0138). These results suggest that the presence and or lack of production of specific antibody to Bb infection may be associated with particular HLA specificities of the Class II.

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Seronegative HIV-2 carriers in India

Cited by 8 publications

References 12 publications

HIV-1/2 differentiation in a South African public laboratory

HIV-1/2 differentiation in a South African public laboratory

Contribution of HLA alleles in the regulation of antibody production in lyme disease

Contribution of HLA alleles in the regulation of antibody production in lyme disease

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