Seroprevalence and B1 gene genotyping of Toxoplasma gondii in farmed European mink in the Republic of Tatarstan, Russia

Shamaev, Nikolai D.; Shuralev, Eduard A.; Petrov, S. V.; Kazaryan, G G; Александрова, Н. М.; Valeeva, Anna R.; Khaertynov, Kamil S.; Mukminov, Malik N.; Kitoh, Katsuya; Takashima, Yasuhiro

doi:10.1016/j.parint.2020.102067

Cited by 5 publications

(10 citation statements)

References 24 publications

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“…T. gondii-infected rodents were also found 2.5 km from the nearest human settlement in a rural area of London, UK 23 . In rural areas, the home-range size of free-ranging domestic cats is wider than that in urban areas [8][9][10][11][12][13] . Thus, it is possible that in the rural areas of the Tatarstan, Russian Federation, stray or free-ranging domestic cats deeply invade the forests and spread T. gondii oocysts in their fecal droppings.…”

Section: Discussionmentioning

confidence: 99%

“…Brain tissue samples were prepared as described previously 12 . Brain samples stored at − 20°С were transferred to a − 86°С deep freezer.…”

Section: Dna Extraction and Pcrmentioning

confidence: 99%

“…Nested PCR was performed with the Takara PCR Ampli cation Kit (Takara Bio Inc., Foster City, California, USA) according to the manufacturer's instructions. The primer sets and PCR conditions used to detect the B1 gene from T. gondii were those described previously 12 .…”

Section: Dna Extraction and Pcrmentioning

confidence: 99%

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Prevalence of Toxoplasma gondii infection among small mammals in Tatarstan, Russian Federation

Shamaev

Shuralev

Nikitin

et al. 2021

Preprint

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Toxoplasma gondii is a zoonotic parasite with a wide host range that includes humans, domestic animals and wild animals. Small mammals serve as intermediate hosts for T. gondii and may contribute to the persistence of this parasite in the environment. Mass mortality in wild animals and deaths in rare endemic species make the study of this parasite of growing importance. In this study, T. gondii infection prevalence was evaluated in brain tissues from 474 small mammals captured at 26 trapping points in urban and rural areas of Tatarstan, Russian Federation. Nested PCR was used to detect the T. gondii B1 gene in the samples. Overall, 40/474 samples (8.44 %) showed B1 gene positivity. T. gondii infection among the wild small mammals trapped in the rural area was significantly higher as a whole than that of the urban area as a whole. Multivariate logistical regression analysis also showed that the trapping area (rural or urban) significantly contributed to T. gondii positivity. Vegetation in the trapping points, small mammal species, sex, age or distance from the trapping points to the nearest human settlements did not significantly affect T. gondii positivity in the sampled small mammals.

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Section: Discussionmentioning

confidence: 99%

“…Brain tissue samples were prepared as described previously 12 . Brain samples stored at − 20°С were transferred to a − 86°С deep freezer.…”

Section: Dna Extraction and Pcrmentioning

confidence: 99%

See 1 more Smart Citation

Prevalence of Toxoplasma gondii infection among small mammals in Tatarstan, Russian Federation

Shamaev

Shuralev

Nikitin

et al. 2021

Preprint

Self Cite

View full text Add to dashboard Cite

show abstract

“…T. gondii -infected rodents were also found 2.5 km from the nearest human settlement in a rural area of London, UK 2 . In rural areas, the home-range size of free-ranging domestic cats is wider than that in urban areas 2 , 8 – 12 . Thus, it is possible that in the rural areas of the Tatarstan, Russian Federation, stray or free-ranging domestic cats deeply invade the forests and spread T. gondii oocysts in their fecal droppings.…”

Section: Discussionmentioning

confidence: 99%

“…Understanding the situation regarding infectious pathogens among wild animals in Tatarstan is therefore worthwhile. We have previously reported the high seroprevalence of toxoplasmosis in humans, cats and domestic animals in Tatarstan, including in the capital city, Kazan 11 , 12 . However, very little is known about T. gondii prevalence among wild animals.…”

Section: Introductionmentioning

confidence: 99%

Prevalence of Toxoplasma gondii infection among small mammals in Tatarstan, Russian Federation

Shamaev

Shuralev

Nikitin

et al. 2021

Sci Rep

Self Cite

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Toxoplasma gondii is a zoonotic parasite with a wide host range that includes humans, domestic animals and wild animals. Small mammals serve as intermediate hosts for T. gondii and may contribute to the persistence of this parasite in the environment. Mass mortality in wild animals and deaths in rare endemic species make the study of this parasite of growing importance. In this study, T. gondii infection prevalence was evaluated in brain tissues from 474 small mammals captured at 26 trapping points in urban and rural areas of Tatarstan, Russian Federation. Nested PCR was used to detect the T. gondii B1 gene in the samples. Overall, 40/474 samples (8.44%) showed B1 gene positivity. T. gondii infection among the wild small mammals trapped in the rural area was significantly higher as a whole than that of the urban area as a whole. Multivariate logistical regression analysis also showed that the trapping area (rural or urban) significantly contributed to T. gondii positivity. Vegetation in the trapping points, small mammal species, sex, age or distance from the trapping points to the nearest human settlements did not significantly affect T. gondii positivity in the sampled small mammals.

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Recent epidemiologic and clinical Toxoplasma gondii infections in wild canids and other carnivores: 2009–2020

Dubey

Murata

Cerqueira‐Cézar

et al. 2021

Veterinary Parasitology

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Seroprevalence and B1 gene genotyping of Toxoplasma gondii in farmed European mink in the Republic of Tatarstan, Russia

Cited by 5 publications

References 24 publications

Prevalence of Toxoplasma gondii infection among small mammals in Tatarstan, Russian Federation

Prevalence of Toxoplasma gondii infection among small mammals in Tatarstan, Russian Federation

Prevalence of Toxoplasma gondii infection among small mammals in Tatarstan, Russian Federation

Recent epidemiologic and clinical Toxoplasma gondii infections in wild canids and other carnivores: 2009–2020

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Seroprevalence and B1 gene genotyping of Toxoplasma gondii in farmed European mink in the Republic of Tatarstan, Russia

Cited by 5 publications

References 24 publications

Prevalence&nbsp;of&nbsp;Toxoplasma&nbsp;gondii&nbsp;infection&nbsp;among&nbsp;small&nbsp;mammals&nbsp;in&nbsp;Tatarstan,&nbsp;Russian&nbsp;Federation

Prevalence&nbsp;of&nbsp;Toxoplasma&nbsp;gondii&nbsp;infection&nbsp;among&nbsp;small&nbsp;mammals&nbsp;in&nbsp;Tatarstan,&nbsp;Russian&nbsp;Federation

Prevalence of Toxoplasma gondii infection among small mammals in Tatarstan, Russian Federation

Recent epidemiologic and clinical Toxoplasma gondii infections in wild canids and other carnivores: 2009–2020

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Prevalence of Toxoplasma gondii infection among small mammals in Tatarstan, Russian Federation

Prevalence of Toxoplasma gondii infection among small mammals in Tatarstan, Russian Federation