Seroprevalence and significance of antibodies to hepatitis C virus in pediatric patients with malignant diseases

Schneppenheim, R.; Rautenberg, Peter; Behnke, Horst; Vietor, K. W.

doi:10.1055/s-2007-1025441

Cited by 5 publications

(1 citation statement)

References 5 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…A review of paediatric German patients (Schneppenheim et a/, 1991) with malignant diseases found a similarly low incidence when testing with second-generation HCV EJA and RIBA (1/82). An Italian study (Dibenedetto et al, 1994) demonstrated a much higher incidence of HCV positivity (using EIA and RIBA), with 32% (28/87) positive results in patients treated between 1986 and 1992.…”

Section: Resultsmentioning

confidence: 99%

Hepatitis C virus infection in multi‐transfused children with haematological malignancy

et al. 1995

View full text Add to dashboard Cite

Transmission of hepatitis C virus (HCV) is an important hazard of blood transfusion and may result in chronic liver disease. 98 children from Nottingham and Sheffield with haematological malignancies were studied to determine the prevalence of HCV infection by enzyme immunoassay and RT/PCR techniques. The children had been exposed to up to 184 donors through red cell and platelet transfusion, the majority prior to routine testing for HCV infection in blood donors. Only one sample showed evidence of HCV infection being both ELISA and RT/PCR positive. None of the samples taken since donor screening were positive. This provides reassurance as to the low rate of HCV acquisition in multi-transfused patients in this part of the U.K. compared to other parts of the world.

show abstract

Section: Resultsmentioning

confidence: 99%

Hepatitis C virus infection in multi‐transfused children with haematological malignancy

et al. 1995

View full text Add to dashboard Cite

show abstract

Immunoblot Detection

Gallagher¹

1996

CP Protein Science

View full text Add to dashboard Cite

Immunoblotting (often referred to as western blotting) is used to identify specific antigens recognized by polyclonal or monoclonal antibodies. Following electrophoretic separation of proteins and transfer from the gel to an appropriate membrane, the immobilized proteins are probed with specific antibodies to identify and quantitate any antigens present. After being probed with primary antibody, the membrane is washed and the antibody-antigen complexes are identified using horseradish peroxidase (HRPO) or alkaline phosphatase (AP) enzymes coupled to the secondary anti-immunoglobulin-G (anti-IgG) antibody (e.g., goat anti-rabbit IgG). As described in this unit, the detection enzymes are attached directly or via an avidin-biotin bridge to the secondary antibody. Chromogenic or luminescent substrates are also described for visualizing the activity.

show abstract