Seroprevalence of Anti-SARS-CoV-2 Antibodies in Chattogram Metropolitan Area, Bangladesh

Ara, Jahan; Islam, Md. Sirazul; Quader, Md. Tarek Ul; Das, Anan; Islam, Mohammad Saiful; Das, Seemanta; Chowdhury, M A Hassan; Das, Goutam; Chowdhury, Sharmin

doi:10.3390/antib11040069

Cited by 4 publications

(3 citation statements)

References 43 publications

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“…Note that positivity thresholds differ slightly between experiment with serum ( Fig 4B ) and whole blood (Figs 4C and 5A–5C ) due to differences in optical absorption of the sample, and are determined separately (Methods). Although seroprevalence across regional populations may exhibit complex spatiotemporal dynamics, our results are in approximate concordance with previous studies of seroprevalence in Bangladesh residents [ 32 , 33 ].…”

Section: Resultssupporting

confidence: 92%

“…For example, a study in the United States [ 39 ] found that 43.7% of adults with “serology results indicative of past infection…reported never having had COVID-19, possibly representing asymptomatic infection”, suggesting that self-reported infection data in general may not correlate well with serological findings. Similarly, previous studies in Bangladesh measured majority seropositivity fractions concordant with our own data [ 32 , 33 ], both of which suggest much higher rates of exposure than indicated by self-reporting.…”

Section: Resultssupporting

confidence: 92%

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A handheld luminometer with sub-attomole limit of detection for distributed applications in global health

Lebel,

Elledge,

Wiener

et al. 2024

PLOS Glob Public Health

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Luminescence is ubiquitous in biology research and medicine. Conceptually simple, the detection of luminescence nonetheless faces technical challenges because relevant signals can exhibit exceptionally low radiant power densities. Although low light detection is well-established in centralized laboratory settings, the cost, size, and environmental requirements of high-performance benchtop luminometers are not compatible with geographically-distributed global health studies or resource-constrained settings. Here we present the design and application of a ~$700 US handheld, battery-powered luminometer with performance on par with high-end benchtop instruments. By pairing robust and inexpensive Silicon Photomultiplier (SiPM) sensors with a low-profile shutter system, our design compensates for sensor non-idealities and thermal drift, achieving a limit of detection of 1.6E-19 moles of firefly luciferase. Using these devices, we performed two pilot cross-sectional serology studies to assess sars-cov-2 antibody levels: a cohort in the United States, as well as a field study in Bangladesh. Results from both studies were consistent with previous work and demonstrate the device’s suitability for distributed applications in global health.

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Section: Resultssupporting

confidence: 92%

Section: Resultssupporting

confidence: 92%

A handheld luminometer with sub-attomole limit of detection for distributed applications in global health

Lebel,

Elledge,

Wiener

et al. 2024

PLOS Glob Public Health

View full text Add to dashboard Cite

show abstract

“…Note that positivity thresholds differ slightly between experiment with serum (Figure 4b) and whole blood (Figure 4c and Figure 5a-c) due to differences in optical absorption of the sample, and are determined separately (Methods). Although seroprevalence across regional populations may exhibit complex spatiotemporal dynamics, our results are in approximate concordance with previous studies of seroprevalence in Bangladesh residents [32,33].…”

Section: Recording Serological Prevalence Of Sars-cov-2 In Bangladeshsupporting

confidence: 92%

A handheld luminometer with sub-attomole limit of detection for distributed applications in global health

Lebel

Elledge²,

Wiener

et al. 2023

Preprint

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Luminescence is ubiquitous in biology research and medicine. Conceptually simple, the detection of luminescence nonetheless faces technical challenges because relevant signals can exhibit exceptionally low radiant power densities. Although low light detection is well-established in centralized laboratory settings, the cost, size, and environmental requirements of high-performance benchtop luminometers are not compatible with geographically-distributed global health studies or resource-constrained settings. Here we present the design and application of a ~$700 US handheld, battery-powered luminometer with performance on par with high-end benchtop instruments. By pairing robust and inexpensive Silicon Photomultiplier (SiPM) sensors with a low-profile shutter system, our design compensates for sensor non-idealities and thermal drift, achieving a limit of detection of 1.6E-19 moles of firefly luciferase, or approximately 1fW of radiant optical power. Using these devices, we performed split luciferase serology studies to monitor sars-cov-2 antibodies in a cohort in the United States, as well as a field study in Bangladesh.

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Twice evasions of Omicron variants explain the temporal patterns in six Asian and Oceanic countries

et al. 2023

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Background The ongoing coronavirus 2019 (COVID-19) pandemic has emerged and caused multiple pandemic waves in the following six countries: India, Indonesia, Nepal, Malaysia, Bangladesh and Myanmar. Some of the countries have been much less studied in this devastating pandemic. This study aims to assess the impact of the Omicron variant in these six countries and estimate the infection fatality rate (IFR) and the reproduction number $${R}_{0}$$ R 0 in these six South Asia, Southeast Asia and Oceania countries. Methods We propose a Susceptible-Vaccinated-Exposed-Infectious-Hospitalized-Death-Recovered model with a time-varying transmission rate $$\beta (t)$$ β ( t ) to fit the multiple waves of the COVID-19 pandemic and to estimate the IFR and $${R}_{0}(t)$$ R 0 ( t ) in the aforementioned six countries. The level of immune evasion and the intrinsic transmissibility advantage of the Omicron variant are also considered in this model. Results We fit our model to the reported deaths well. We estimate the IFR (in the range of 0.016 to 0.136%) and the reproduction number $${R}_{0}(t)$$ R 0 ( t ) (in the range of 0 to 9) in the six countries. Multiple pandemic waves in each country were observed in our simulation results. Conclusions The invasion of the Omicron variant caused the new pandemic waves in the six countries. The higher $${R}_{0}(t)$$ R 0 ( t ) suggests the intrinsic transmissibility advantage of the Omicron variant. Our model simulation forecast implies that the Omicron pandemic wave may be mitigated due to the increasing immunized population and vaccine coverage.

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Seroprevalence of Anti-SARS-CoV-2 Antibodies in Chattogram Metropolitan Area, Bangladesh

Cited by 4 publications

References 43 publications

A handheld luminometer with sub-attomole limit of detection for distributed applications in global health

A handheld luminometer with sub-attomole limit of detection for distributed applications in global health

A handheld luminometer with sub-attomole limit of detection for distributed applications in global health

Twice evasions of Omicron variants explain the temporal patterns in six Asian and Oceanic countries

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