Seroprevalence of Antibodies against Highly Pathogenic Avian Influenza A (H5N1) Virus among Poultry Workers in Bangladesh, 2009

Nasreen, Sharifa; Khan, Salah Ud‐Din; Azziz‐Baumgartner, Eduardo; Hancock, Kathy; Veguilla, Vic; Wang, David; Rahman, Mahmudur; Alamgir, A. S. M.; Sturm‐Ramirez, Katharine; Gurley, Emily S.; Luby, Stephen P.; Katz, Jacqueline M.; Uyeki, Timothy M.

doi:10.1371/journal.pone.0073200

Cited by 28 publications

(22 citation statements)

References 27 publications

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“…Similar has been reported in various studies previously. 17,30,41,42 Although H5N1 has persistently circulated in poultry in many other countries, especially in Asia, human infection has rarely been reported. 24,[43][44][45][46] H5 viruses have shown strong host specificity for infection, which could be the reason for the low infection rate in poultry exposed people even in countries enzootic for the disease.…”

Section: Discussionmentioning

confidence: 99%

Avian influenza at animal‐human interface: One‐health challenge in live poultry retail stalls of Chakwal, Pakistan

Chaudhry

Webby

Swayne

et al. 2020

Influenza Resp Viruses

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Background: Live poultry retail stalls (LPRSs) are believed to be the source of human infection with avian influenza viruses (AIVs); however, little is known about epidemiology of these viruses in LPRSs of Pakistan. Objectives:The current study was conducted to estimate the virological and serological prevalence of AIVs in humans and poultry and associated risk factors among seropositive butchers. Methods: A field survey of LPRSs of Chakwal District was conducted betweenDecember 2015 and March 2016. In total, 322 samples (sera = 161 and throat swab = 161) from butchers and 130 pooled oropharyngeal swabs and 100 sera from birds were collected. Baseline sera (n = 100) from general population were also tested.Data were collected by structured questionnaires. Sera were tested by hemagglutination inhibition (HI) test further confirmed by micro-neutralization test (MN). Swabs were processed by real-time RT-PCR. Logistic regression analyses were conducted to identify risk factors. Results:In butchers, 15.5% sera were positive for antibodies against H9 virus using a cutoff of ≥40 in HI titer; 6% sera from general population were positive for H9.Seroprevalence in poultry was 89%, and only 2.30% swabs were positive for H9.Presence of another LPRS nearby and the number of cages in the stall were risk factors (OR > 1) for H9 seroprevalence in butchers. Conclusions:This study provides evidence of co-circulation of H9 virus in poultry and exposure of butchers in the LPRSs, which poses a continued threat to public health.We suggest regular surveillance of AIVs in occupationally exposed butchers and birds in LPRSs.

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Section: Discussionmentioning

confidence: 99%

Avian influenza at animal‐human interface: One‐health challenge in live poultry retail stalls of Chakwal, Pakistan

Chaudhry

Webby

Swayne

et al. 2020

Influenza Resp Viruses

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“…As far as we are aware, similar problem has not been reported. This is probably because most serological studies usually use only one test, either HI test or ELISA (Nasreen et al 2013;Chang et al 2014). A possible reasons is that HI, MM2e ELISA and NP ELISA all measure different type of antibodies and it is likely that the duration of these antibodies in serum following infection also differ.…”

Section: Discussionmentioning

confidence: 99%

Circulating H5N1 virus among native chicken living around commercial layer farms

Tarigan¹,

Indriani²,

Ignjatovic³

2015

JITV

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ABSTRAK Tarigan Sejak diterapkannya program vaksinasi kasus penyakit avian influenza (AI) H5N1 pada ayam pembibitan dan ayam ras petelur jarang terdengar. Penelitian in bertujuan menganalisis apakah redanya kasus tersebut berhubungan dengan hilangnya sumber infeksi di sekitar peternakan. Sampel serum dikumpulkan dari 421 ayam buras yang tinggal di sekitar peternakan ayam petelur di Kabupaten Cianjur dan Sukabumi, Jawa Barat pada Maret-April 2014. Antibodi virus AI H5N1 dianalisis dengan uji haemagglutination ihibition (HI), ELISA dan immunoblotting untuk mendeteksi antibodi terhadap haemagglutin virus H5N1, domain eksternal protein M2 (M2E) dan nukleoprotein (NP) virus AI. Sebanyak 8,6% dari ayam buras yang diperiksa seropositif terhadap virus AI berdasarkan satu atau lebih dari uji serologis. Hasil penelitian mengungkapkan bahwa virus H5N1 masih beredar pada ayam buras yang berkeliaran disekitar kandang ayam ras petelur. Sera yang positif dengan uji HI, M2E dan NP ELISA berturut turut 2,4%, 3,3% dan 3,8%. Tidak terlihat kesesuaian antara hasil satu uji dengan uji lainya. Penyebab ketidaksesuaian hasil tersebut diduga karena HI test, MM2e ELISA dan NP ELISA mengukur antibody yang berbeda yang kemunculan dan durasi masing masing antibodi tersebut berbeda. Kenyataan bahwa virus H5N1 masih beredar di sekitar peternakan ayam petelur menunjukkan bahwa ancaman virus AI masih membayangi peternakan ayam komersial dan karena itu vaksinasi dan biosekuriti yang ketat masih dibutuhkan. Soon after the application of vaccination programme against high pathogenic avian influenza H5N1 outbreak of the disease in breeder and commercial layer farms has diminished remarkably in West Java. This study aimed to investigate whether the H5N1 decline is related to the disappearance of source of infection around the farms. Serum samples were collected from 421 native chicken living around commercial layer farms in the Districs of Cianajur and Sukabumi, West Java in March-April 2014. Antibodies to avian influenza virus (AIV) H5N1 were measured using haemaglutination inhibition (HI), ELISAs and immunoblotting that measured presence of antibodies to the haemagglutin of H5N1 strain, as well as the M2e and nucleoprotein (NP) of all avian influenza viruses. Based on the combined results, 8.6% of the native chickens were seropositive to AI virus based on one or more of serological tests. This study provided serological evidence that H5N1 virus was still circulating among native chicken living around commercial layer farms. Many positive sera were however positive for antibodies in one test only: 2.4%, 3.3% and 3.8% by HI test, M2e and NP ELISA, respectively. It could be speculated that the incongruity of the results is due to the fact that HI, MM2e ELISA and NP ELISA all measure different type of antibodies and the duration of these antibodies in serum following infection with H5N1 differ. The fact that H5N1 virus is still circulating around commercial layer farms infers that the commercial farms are still under threat and therefore vaccination ...

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“…Recent studies suggest that H5N1 virus have the potential to acquire airborne transmission in mammals (Herfst et al, 2012; Imai et al, 2012). Furthermore, humans are largely serologically naive to the H5N1 subtype based on the available H5N1 seroepidemiologic studies (Liao et al, 2013; Nasreen et al, 2013; Van Kerkhove, 2013). In addition, newly isolated HPAI H5 viruses have recently caused outbreaks in wild birds and domestic poultry in Asia, Europe, and North America (Jhung et al, 2015; Smith et al, 2015; WHO, 2015b).…”

Section: Introductionmentioning

confidence: 99%

A cationic liposome–DNA complexes adjuvant (JVRS-100) enhances the immunogenicity and cross-protective efficacy of pre-pandemic influenza A (H5N1) vaccine in ferrets

et al. 2016

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Influenza A (H5N1) viruses continue to pose a public health threat. As inactivated H5N1 vaccines are poorly immunogenic, adjuvants are needed to improve the immunogenicity of H5N1 vaccine in humans. Here, we investigated the immunogenicity and cross-protective efficacy in ferrets of a clade 2.2-derived vaccine with addition of JVRS-100, an adjuvant consisting of cationic liposome–DNA complexes (CLDC). After the first vaccination, significantly higher levels of hemagglutination-inhibition (HAI) and neutralizing antibody titers were detected in ferrets immunized with adjuvanted vaccine compared to unadjuvanted vaccine. Following a second dose of adjuvanted vaccine, HAI antibody titers of ≥40 were detected against viruses from multiple H5N1 clades. HAI antibodies against newly isolated H5N2 and H5N8 viruses were also augmented by JVRS-100. Ferrets were challenged with a heterologous H5N1 virus. All ferrets that received two doses of adjuvanted vaccine exhibited mild illness, significantly reduced nasal wash virus titers and protection from lethal challenge. In contrast, ferrets that received unadjuvanted vaccine showed greater weight loss, high viral titers and 3 of 6 animals succumbed to the lethal challenge. Our results indicate that the addition of JVRS-100 to H5N1 vaccine enhanced immunogenicity and cross-protection against lethal H5N1 virus disease in ferrets. JVRS-100 warrants further investigation as a potential adjuvant for influenza vaccines.

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Seroprevalence of Antibodies against Highly Pathogenic Avian Influenza A (H5N1) Virus among Poultry Workers in Bangladesh, 2009

Cited by 28 publications

References 27 publications

Avian influenza at animal‐human interface: One‐health challenge in live poultry retail stalls of Chakwal, Pakistan

Avian influenza at animal‐human interface: One‐health challenge in live poultry retail stalls of Chakwal, Pakistan

Circulating H5N1 virus among native chicken living around commercial layer farms

A cationic liposome–DNA complexes adjuvant (JVRS-100) enhances the immunogenicity and cross-protective efficacy of pre-pandemic influenza A (H5N1) vaccine in ferrets

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