Seroprevalence of Bovine Immunodeficiency Virus and Bovine Leukemia Virus in Dairy and Beef Cattle in Hokkaido.

Usui, Tatsufumi; Meas, Sothy; Konnai, Satoru; Ohashi, Kazuhiko; Onuma, Misao

doi:10.1292/jvms.65.287

Cited by 15 publications

(10 citation statements)

References 19 publications

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“…Although BIV infected cattle have been identified in some countries neighboring Iran [4,19], to the best of our knowledge this is the first report of BIV infection in Iranian Holstein cattle. Although we studied only a small number of animals, the prevalence we found of 20.3% is similar to that reported from other Asian countries (12% in Turkey [19] to 35% in Korea [20]), and neighboring countries such as Pakistan (16 %) [4,13]. The prevalence of BLV infection (17%) in the current study was also similar to that of other Asian and non Asian countries (9 to 33 %) [21][22][23][24].…”

Section: Discussionsupporting

confidence: 86%

“…Following experimental infections, cattle may have transient increases in lymphocytes, lymphoid hyperplasia, atypical lymphosarcoma, and secondary bacterial infections [6,10,11]. It has also been suspected that the stress of parturition in BIV infected cows is associated with the progression of bovine leukemia virus (BLV) infections [8,12,13].…”

Section: Introductionmentioning

confidence: 99%

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Bovine immunodeficiency virus and bovine leukemia virus and their mixed infection in Iranian Holstein cattle

Brujeni

Poorbazargani

Nadin-Davis

et al. 2010

J Infect Dev Ctries

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Introduction: Bovine immunodeficiency virus (BIV) and bovine leukemia virus (BLV) have worldwide distributions, but their prevalences in Iran are unknown. We investigated the presence of infections in Iranian Holstein cattle and determined changes in hematological values for infected animals. Methodology: Nested PCR was used on blood samples from 143 animals Holstein cattle to detect proviral BIV and BLV gag sequences. Flow cytometric analysis was performed using monoclonal antibodies (mAbs) against CD4, CD8, and CD21 bovine T lymphocyte subsets. Results: Proviral BIV and BLV gag sequences were detected in 20.3% and 17% of the animals, respectively. BIV-BLV confection was also detected in 4.2% of the study population but this was not statistically significant. Flow cytometric analysis showed that both BIV-infected cows and non-infected ones had CD4/CD8 ratios of 2.45 and 1.43, respectively, and this difference was significant. BLV infected and non-infected animals had no significant differences in their CD4/CD8 ratio. In comparison to non-infected cattle, those with both BIV and BLV had a significant decrease in their CD4/CD8 ratios (1.5 % vs. 2.3; P = 0.01). Conclusion: This is the first report of BIV and BLV infections in Iran. We found no evidence that infection with one agent predisposed an animal to infection with the other. BIV infection may have a role in decreasing T CD8 counts, but this may depend on the genetics of the cattle and virus strains involved.

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Section: Discussionsupporting

confidence: 86%

Section: Introductionmentioning

confidence: 99%

Bovine immunodeficiency virus and bovine leukemia virus and their mixed infection in Iranian Holstein cattle

Brujeni

Poorbazargani

Nadin-Davis

et al. 2010

J Infect Dev Ctries

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“…The cow was fertilized naturally, and the calf was placed in conventional housing with the dam, and monitored for the presence of BLV-specific antibodies, as previously described [24,26,27]. Whole blood samples and sera were collected at 3-week intervals.…”

Section: Methodsmentioning

confidence: 99%

“…With the exception of a few European countries, EBL is considered to have a worldwide distribution. Although the majority of infected cattle remain clinically asymptomatic, invisible losses in productivity have a significant economic impact on the dairy industry [4,24,26]. BLV is an oncogenic retrovirus of the Retroviridae family.…”

Section: Introductionmentioning

confidence: 99%

Agar gel immunodiffusion analysis using baculovirus-expressed recombinant bovine leukemia virus envelope glycoprotein (gp51/gp30T-)

et al. 2009

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Bovine leukemia virus (BLV) envelope glycoprotein (gp51/gp30T-), consisting of BLV gp51 and BLV gp30 that lacked its C-terminal transmembrane domain, was expressed in insect cells under the control of the baculovirus polyhedron promoter. Recombinant BLV gp51/gp30T- secreted from insect cells was determined by immunofluorescence, enzyme-linked immunosorbent and western blot assays using a BLV-specific monoclonal antibody and BLV-positive bovine antibodies. An agar gel immunodiffusion (AGID) test using gp51/gp30T- as the antigen for the detection of BLV antibodies in serum was developed and compared to traditional AGID, which uses wild type BLV antigen derived from fetal lamb kidney cells. AGID with the recombinant BLV gp51/gp30T- was relatively more sensitive than traditional AGID. When the two methods were tested with bovine sera from the field, the recombinant BLV gp51/gp30T- and traditional antigen had a relative sensitivity of 69.8% and 67.4%, respectively, and a relative specificity of 93.3% and 92.3%. These results indicated that the recombinant BLV gp51/gp30T- is an effective alternative antigen for the diagnosis of BLV infection in cattle.

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“…Meas and collaborators reported that env gene sequences from circulating viruses in Japan (Usui, Meas, Konnai, Ohashi, & Onuma, ), Pakistan (Meas, Seto, et al, ) and Cambodia (Meas, Ohashi, et al, ) were shorter and presented more substitutions compared to isolates from the USA (R29, Florida 112 and Oklahoma 40). These regions remain unknown for circulating viruses in Mexico.…”

Section: Resultsmentioning

confidence: 99%

First evidence of bovine immunodeficiency virus infection in Mexican cattle

González-Fernández

Pérez

Flores

et al. 2020

Transbound Emerg Dis

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This study set out to identify the presence of bovine immunodeficiency virus (BIV) in animals geographically located in Mexico. BIV was first discovered in the United States in a dairy cow with persistent lymphocytosis, lymphoid hyperplasia and lymphocytic encephalitis. Many studies indicate that BIV infection is globally distributed, but its presence in Mexico remains unknown. We collected 1,168 heparinized blood samples from cattle in ten states across the Mexican Republic, then separated plasma using centrifugation and tested for antibodies against BIV. We used an indirect ELISA based on the use of a synthetic peptide derived from transmembrane glycoprotein (gp45/TM). In order to identify the viral genome, we designed a synthetic gene as a PCR control, as well as a pair of oligonucleotides for amplifying a 519 bp product of the env gene which encodes the surface protein. Positive amplicons were purified and subjected to nucleotide sequencing. A total of 189 (28.94%) tested plasma samples suggest the presence of specific anti‐BIV antibodies in all states studied except for Chiapas. Additionally, PCR results identified six positive cows in the states of Puebla and Coahuila. BIV in these cows was confirmed via nucleotide sequencing and in silico analysis of these samples. This is the first report of the presence of BIV in Mexican cattle.

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Seroprevalence of Bovine Immunodeficiency Virus and Bovine Leukemia Virus in Dairy and Beef Cattle in Hokkaido.

Cited by 15 publications

References 19 publications

Bovine immunodeficiency virus and bovine leukemia virus and their mixed infection in Iranian Holstein cattle

Bovine immunodeficiency virus and bovine leukemia virus and their mixed infection in Iranian Holstein cattle

Agar gel immunodiffusion analysis using baculovirus-expressed recombinant bovine leukemia virus envelope glycoprotein (gp51/gp30T-)

First evidence of bovine immunodeficiency virus infection in Mexican cattle

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