Seroprevalence of Chlamydia abortus and Brucella spp. and risk factors for Chlamydia abortus in pigs from China

Zhang, Huaiyu; Zhang, Zhixiang; Li, Yunhui; Li, Wei; Jin, Youshun; Li, Zhaocai; Zhou, Jizhang; Tong, Dewen

doi:10.1016/j.actatropica.2023.107050

Cited by 5 publications

(1 citation statement)

References 35 publications

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“…Chlamydia abortus (C. abortus) is a pathogen that can cause ovine enzootic abortion in goats, sheep, pigs, and cattle, resulting in significant economic losses to the global livestock industry and posing a great public health safety risk (Longbottom et al, 2002). The infection of pregnant animals with C. abortus usually results in preterm, stillborn, or mummified fetuses, whereas the majority of other infected animals show asymptomatic or subclinical signs, which increases the risk of pathogen transmission between herds (Zhang et al, 2023). Therefore, safe and effective vaccines are the most efficient way to effectively control the Zhang et al 10.3389/fmicb.2024.1349746 Frontiers in Microbiology 02 frontiersin.org infection.…”

Section: Introductionmentioning

confidence: 99%

Comparison of the immune effects of the Chlamydia abortus MOMP antigen displayed in different parts of bacterial ghosts

Zhang,

Li,

et al. 2024

Front. Microbiol.

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Bacterial ghosts (BGs) are promising vaccine platforms owing to their high adjuvant properties and delivery efficiency. Heterologous antigens can be anchored to different parts of BGs using genetic engineering strategies to prepare vaccines. However, several key issues need to be resolved, including the efficient preparation of BGs and determining the optimal anchoring position of exogenous antigens in the BGs. Here, we prepared an efficient temperature-controlled lysis system using lysis gene E of phage PhiX174 and used the major outer membrane protein (MOMP) of Chlamydia abortus (C. abortus) as a model antigen to explore the optimal display location of exogenous antigens in BGs. We demonstrated that the constructed recombinant temperature-controlled lysis plasmid can still stably inhibit E gene expression at 37°C, and the lysis efficiency of E. coli can reach above 99.9%. Four recombinant MOMP Escherichia coli (E. coli) ghost vaccines were constructed using different anchor sequences. These vaccines all induced strong specific antibody responses and secrete high levels of IFN-γ in immunized mice and significantly increased the clearance of C. abortus in a mouse infection model. Notably, the strongest immune effect was observed when MOMP was displayed on the surface of E. coli ghosts (rECG-InpN-M), which resulted in the clearance of C. abortus in mice 6 days earlier than that with the recombinant MOMP vaccine. Altogether, we constructed an efficient BG temperature-controlled lysis system and provided a feasible strategy for developing a BG delivery platform with enhanced immune effects.

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Section: Introductionmentioning

confidence: 99%

Comparison of the immune effects of the Chlamydia abortus MOMP antigen displayed in different parts of bacterial ghosts

Zhang,

Li,

et al. 2024

Front. Microbiol.

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Features Responsible for Spread of Zoonotic Disease Brucella: A Study on Central India Population

Namdev,

Makhijani,

Dorshetwar

et al. 2024

SN COMPUT. SCI.

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Seroprevalence and risk factors of Chlamydia abortus infection in Tibetan sheep in Qinghai province, China

Lei,

Yu,

Niu

et al. 2024

Preprint

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Background Chlamydia abortus (C. abortus) is Gram-negative, intracellular bacterium, causing ovine enzootic abortion (OEA). No data about C. abortus infection are available in Tibetan sheep in Qinghai, China. Result A total of 1043 Tibetan sheep serum samples were collected from 8 Tibetan sheep flocks in Qinghai, China, then were detected for specific antibodies against C. abortus using a commercial ELISA Kit. The results showed that the overall seroprevalence of C. abortus in Tibetan sheep was 153/1043 (14.7%, 95% confidence interval [CI] 12.5–16.8). The prevalence in female and male Tibetan sheep was 58/421 (13.8%, 95% CI 10.5 ~ 17.1) and 95/622 (15.3%, 95% CI 18.6 ~ 26.6), respectively with no significant difference (P > 0.05). The seroprevalence of antibodies to C. abortus in Tibetan sheep ranged from 10.0 to 21.7% among the eight different areas, and the difference was also without statistical significance [1]. The prevalence among different age groups ranged from 14.0 to 16.5%, with a higher prevalence among 1 to 1.5 years age category. Conclusion The results demonstrate the presence of C. abortus infection in Tibetan sheep in Qinghai and its potential impact on public health and the local Tibetan economy. To our knowledge, this is the first seroprevalence survey of C. abortus in Tibetan sheep in Qinghai, China.

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Seroprevalence of Chlamydia abortus and Brucella spp. and risk factors for Chlamydia abortus in pigs from China

Cited by 5 publications

References 35 publications

Comparison of the immune effects of the Chlamydia abortus MOMP antigen displayed in different parts of bacterial ghosts

Comparison of the immune effects of the Chlamydia abortus MOMP antigen displayed in different parts of bacterial ghosts

Features Responsible for Spread of Zoonotic Disease Brucella: A Study on Central India Population

Seroprevalence and risk factors of Chlamydia abortus infection in Tibetan sheep in Qinghai province, China

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