Seroprevalence of Classical Swine Fever in pigs of Karnataka and comparative diagnostic evaluation of antigen ELISA and reverse transcriptase -PCR

Abstract

Classical swine fever is highly contagious viral disease of swine causing huge economic losses to pig farmersand pig industry. The study was conducted in Karnataka to understand the current scenario of CSF in the state andto evaluate efficiency of diagnostic tests (antigen ELISA and RT-PCR) in detecting the disease. Serum sampleswere collected from 270 pigs from 13 districts of the state and were tested for the presence of CSF antibodies.Whole blood samples (151) from 14 outbreaks of CSF were collected for the comparative diagnosis of the diseaseusing Antigen ELISA and reverse transcriptase (RT)-PCR. A seroprevalence of 28.5% (77/270) was found in theserum samples collected form the whole Karnataka. The southern Karnataka had higher seroprevalence (47%) incomparison to northern Karnataka region where seroprevalence was 17%. It confirms endemicity of the disease insouthern region. Of 151 blood samples collected for comparative diagnosis, 61 samples were positive for CSF byRT-PCR and 39 by antigen ELISA, indicating the superiority of RT-PCR over antigen ELISA to detect CSFVinfection in earliest stages of infection.