Seroprevalence of Entamoeba histolytica Infection in China

Abstract

Abstract. The seroprevalence of Entamoeba histolytica infection in the residents of seven provinces in China was examined by using an enzyme-linked immunosorbent assay with a crude antigen and a recombinant surface antigen, C-Igl, of the parasites. A total of 1,312 serum samples were investigated. The positivity rates for these two antigens were 11.05% and 6.25%, respectively. There was no significant difference in the seropositivity to E. histolytica between men and women. We used a logistic regression model and maximal-likelihood methods to estimate the prevalence of E. histolytica infection from sequential serologic data. Seropositivity in Sichuan, Guizhou, and Sinkiang Provinces was higher than that in Beijing, Shanghai, and Qinghai Provinces. The present study provides an overview of seropositivity to E. histolytica infection in seven provinces in China and use the logistic regression model estimation method to achieve a more accurate measure of amebiasis prevalence.

Serological Surveillance Development for Tropical Infectious Diseases Using Simultaneous Microsphere-Based Multiplex Assays and Finite Mixture Models

“…Seropositivities in all age groups were higher in our study areas compared with the Chinese population, in which seropositivities ranged from 3–8% [36]. Comparing the seropositivities among children from our two study sites, the rates for children from Mbita were generally higher than those for children from Kwale.…”

“…For the assay for E. histolytica , the recombinant surface antigen C-IgL was previously validated [12] and was used for seroepidemiology of E. histolytica in Chinese populations [36]. Seropositivities in all age groups were higher in our study areas compared with the Chinese population, in which seropositivities ranged from 3–8% [36].…”

Primary pathogenicity analysis of a Chinese Entamoeba histolytica isolate

“…Brief Report survey of HIV-positive patients in China showed a serum-positive rate of 7.9% for E. histolytica (4). A recent study in seven provinces in China has shown a serum-positive rate of 0.53% to 9.04% for E. histolytica (5). However, insufficient pathogenic information on Chinese E. histolytica strains is currently available.…”

“…The trophozoites (2 × 10 4 ) were incubated with 10 5 Jurkat cells (The Cell Bank of Chinese Academy of Science, Shanghai, China) for 20 min at 37°C. The cells were then washed twice and stained with FITCconjugated annexin V (Sigma-Aldrich).…”

Increases in Entamoeba histolytica Antibody–Positive Rates in Human Immunodeficiency Virus–Infected and Noninfected Patients in Japan: A 10-Year Hospital-Based Study of 3,514 Patients

“…In Japan, invasive amebiasis is an emerging sexually transmitted parasitic disease, increasing alongside the incidence rates of other sexually transmitted infections (STIs) including human immunodeficiency virus (HIV), chlamydial, and gonococcal infections 14,15. Previous studies have indicated that HIV infection is a risk factor for invasive amebiasis 11,13,16,17. However, epidemiological data on the trends of invasive amebiasis in patients with and without HIV infection are scarce.…”