Seroprevalence of Japanese enchephalitis Infection in Pigs in Tulungagung, East Java

Widiastuti, Dyah; Wijayanti, Tri; Isnani, Tri; Pramestuti, Nova; Wijayanti, Siwi Mars Pramatama

doi:10.22435/blb.v15i2.1888

Cited by 3 publications

(4 citation statements)

References 15 publications

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“…The research of Ompusungu obtained seroprevalence of all pig samples in Indonesia of 14.2%. Seroprevalence of JEV in pigs in Tulungagung, East Java was 19.2% [21].…”

Section: Discussionmentioning

confidence: 95%

Seroprevalence of Japanese Encephalitis Virus in Pigs and Cattle: An Evaluation from Tangerang Regency

Riandi¹,

Ipa²,

Wahono³

et al. 2023

Proceedings of the 1st International Conference for Health Research – BRIN (ICHR 2022)

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Japanese encephalitis (JE) is a zoonotic vector-borne disease in humans and animals in the Asia Pacific. This RNA virus is transmitted to humans through mosquito bites from viral amplifiers, such as pigs, poultry, bats, and other vertebrates. In the spread of JEV, herons are considered the reservoir, pigs as the main amplifying host, and the Culex mosquito as a vector. Although the number of cases of JE in humans is low, Indonesia is considered an endemic area for JE. The purpose of this study was to evaluate the seroprevalence of JE in pigs and cattle in Tangerang Regency, the area with the largest pig population in Banten Province. From June -to July 2019, a total of 101 samples of pig blood and 110 samples of cattle blood were collected from 13 pig farms and 42 cattle farms. Characteristics of livestock management were recorded for further descriptive analysis. From 101 samples of pig blood and 110 samples of cattle blood, only 94 pig serum and 94 cattle serum were obtained for testing by Japanese encephalitis virus (JEV) Specific IgG ELISA; the seroprevalence results for pigs were 6.38% and 7.44% for cattle. The follow-up PCR test for porcine and bovine serum found none of the samples with the JE virus. Based on seroprevalence results and livestock management characteristics, we suspect that the JE antibody in reactive pig serum was inherited from their sow before being imported from outside the Tangerang district. However, judging from the location of the source of the positive JEV IgG cattle, almost all of which were obtained locally, there is a possibility that JEV transmission occurred in Tangerang Regency. We suggest the need for JE virus control in breeding farms in the area of origin of pigs that enter the Tangerang Regency area, considering that the introduction of the virus can occur through the transportation of cattle or piglets between regions.

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“…The research of Ompusungu obtained seroprevalence of all pig samples in Indonesia of 14.2%. Seroprevalence of JEV in pigs in Tulungagung, East Java was 19.2% [21].…”

Section: Discussionmentioning

confidence: 95%

Seroprevalence of Japanese Encephalitis Virus in Pigs and Cattle: An Evaluation from Tangerang Regency

Riandi¹,

Ipa²,

Wahono³

et al. 2023

Proceedings of the 1st International Conference for Health Research – BRIN (ICHR 2022)

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“…Infection in pigs was higher (23.1%) on farms found Culex larvae. [19] The catch of mosquitoes in the pig farm in the Ngunut area was dominated by Anopheles vagus (53.4%), Culex vishnui (14.2%), and Cx. quenquefasciatus (12.7%), although no JE positive test results were found in mosquito samples.…”

Section: Fig 2 Contour Maps Agricultural Land and Rivers In The District Tulungagungmentioning

confidence: 99%

Ecohealth Approach in Anticipating Japanese Encephalitis in Tulungagung District, East Java Province

et al. 2021

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Japanese-B-encephalitis (JE) is a disease caused by the Japanese encephalitis virus from the Flavivirus group, the Flaviviridae family. In Indonesia, JE cases have not been widely reported in both animals and humans, although encephalitis in humans is common. Yamanaka reported positive for anti-JE antibodies in 96 (6%) pigs in Tulungagung District. Environmental elements need to be identified to support the establishment of a healthy ecosystem to minimize the potential of JE transmission. This study aims to identify the environment that can support JE transmission and conceptualize possible approaches. The method is carried out by conducting environmental observations of risky environmental conditions in April November 2016 and searching for supporting literature. The results showed that there were many puddles in the environment of chicken and pig farms. Besides that, there were many open puddles of household waste in the residential areas. This place is the habitat for Culex Sp. mosquitoes. Another research reported 19.05% of pigs who were positive for Ab JE in Tulungagung District. Environmental improvement can be done by cultivating fish that is resistant to dirty water, for example, catfish in ponds in chicken and pig farms and the construction of closed disposal facilities.

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“…Previously, several serological surveys have been conducted on pig farms and wild boars, which tend to show high seropositivity in different regions of the world, including China [ 14 , 15 , 16 , 17 , 18 ]. As these animals live apart from human populations, serosurveys of pigs and wild animals may not indicate the prevalence of JEV in urban/residential areas.…”

Section: Introductionmentioning

confidence: 99%

“…The JEV genome is approximately 11 kb in length and is proteolytically processed into three structural (Cap, prM, and E) and seven non-structural (NS1, NS2A, NS2B, NS3, NS4A, NS4B, and NS5) proteins by a complex combination of host and viral proteases [5][6][7][8]. Phylogenetically, JEV is classified into a single serotype with five genetically different Viruses 2024, 16, 258 2 of 12 genotypes (GI, GII, GIII, GIV, and GV). JEV GIII had been the most dominant strain, with several outbreaks in past.…”

Section: Introductionmentioning

confidence: 99%

Development of Colloidal Gold-Based Immunochromatographic Strips for Rapid Detection and Surveillance of Japanese Encephalitis Virus in Dogs across Shanghai, China

Zhong,

Wahaab,

Zheng

et al. 2024

Viruses

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Japanese encephalitis virus (JEV) causes acute encephalitis in humans and is of major public health concern in most Asian regions. Dogs are suitable sentinels for assessing the risk of JEV infection in humans. A neutralization test (NT) or an enzyme-linked immunosorbent assay (ELISA) is used for the serological detection of JEV in dogs; however, these tests have several limitations, and, thus, a more convenient and reliable alternative test is needed. In this study, a colloidal gold immunochromatographic strip (ICS), using a purified recombinant EDIII protein, was established for the serological survey of JEV infection in dogs. The results show that the ICSs could specifically detect JEV antibodies within 10 min without cross-reactions with antibodies against other canine viruses. The test strips could detect anti-JEV in serum with dilution up to 640 times, showing high sensitivity. The coincidence rate with the NT test was higher than 96.6%. Among 586 serum samples from dogs in Shanghai examined using the ICS test, 179 (29.98%) were found to be positive for JEV antibodies, and the high seropositivity of JEV in dogs in China was significantly correlated with the season and living environment. In summary, we developed an accurate and economical ICS for the rapid detection of anti-JEV in dog serum samples with great potential for the surveillance of JEV in dogs.

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Seroprevalence of Japanese enchephalitis Infection in Pigs in Tulungagung, East Java

Cited by 3 publications

References 15 publications

Seroprevalence of Japanese Encephalitis Virus in Pigs and Cattle: An Evaluation from Tangerang Regency

Seroprevalence of Japanese Encephalitis Virus in Pigs and Cattle: An Evaluation from Tangerang Regency

Ecohealth Approach in Anticipating Japanese Encephalitis in Tulungagung District, East Java Province

Development of Colloidal Gold-Based Immunochromatographic Strips for Rapid Detection and Surveillance of Japanese Encephalitis Virus in Dogs across Shanghai, China

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