Seroprevalence of Leptospirosis among Water Buffaloes, Pigs, and dogs in Selected Areas in the Philippines, 2007 to 2008

Villanueva, Sharon Y. A. M.; Baterna, Rubelia A.; Cavinta, Lolita L.; Yanagihara, Yasutake; Gloriani, Nina G.; Yoshida, Shigeaki

doi:10.47895/amp.v52i1.493

Cited by 3 publications

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“…3B). Among the articles that performed MAT or Schuffner–Mochtar agglutination-lysis tests (precursor of MAT), only 16.33% (8/49) stated information about the strains used for MAT (Thiermann, 1980; Rodríguez et al ., 2004; Adesiyun et al ., 2006; Jung et al ., 2008; Roach et al ., 2010; Cruz-Ramero et al ., 2013; Benacer et al ., 2017; Villanueva et al ., 2018). Among the articles that exhibited at least one positive animal on MAT or Schuffner–Mochtar agglutination-lysis test, 16.33% (8/49) showed their results as serogroups (Ryu, 1975; Farrington and Sulzer, 1982; Weeks et al ., 1997; Ivana et al ., 2010; Desvars et al ., 2012, Desvars et al ., 2013; Benacer et al ., 2017; Miotto et al ., 2018).…”

Section: Resultsmentioning

confidence: 99%

Canine leptospirosis in stray and sheltered dogs: a systematic review

Costa

Colocho

Pereira

et al. 2022

Anim. Health. Res. Rev.

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The aim of this systematic review was to investigate the prevalence of leptospirosis among stray and sheltered dogs worldwide. Six databases were searched, which resulted in the retrieval of 476 articles. Sixty articles were selected for analysis according to 10 quality criteria. Among the selected papers, 26 papers [43.4% (26/60)] met five of the 10 quality criteria established, 10 papers [16.7% (10/60)] met three criteria, nine papers [15.0% (9/60)] met four criteria, six papers [10.0% (6/60)] met six criteria, four papers [6.7% (4/60)] met eight criteria, and three papers [5.0% (3/60)] met nine of the 10 criteria, whereas two papers [1.7% (1/60)] met two and seven [1.7% (1/60)] criteria. Publications originated mainly from the Americas [45.0% (27/60)] and in the last 16 years (2003–2019) [81.7% (49/60)], and most of the sampled dogs were stray dogs [65.0% (39/60)]. The most commonly used diagnostic test for leptospirosis was the microscopic agglutination test [78.4% (47/60)] followed by polymerase chain reaction [21.7% (13/60)], and the most common serovars were Canicola [71.4% (35/49)], Icterohaemorrhagiae [65.3% (32/49)], Grippotyphosa [40.8% (20/49)], and Pomona [40.8% (20/49)]. In conclusion, our results showed that Leptospira spp. are present in unowned dogs worldwide; however, the low-methodological quality of the recovered cross-sectional studies precluded a meta-analysis.

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Section: Resultsmentioning

confidence: 99%

Canine leptospirosis in stray and sheltered dogs: a systematic review

Costa

Colocho

Pereira

et al. 2022

Anim. Health. Res. Rev.

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show abstract

“…3 Currently, studies are focused on the need for continued monitoring of the prevailing serovars in a given geographical area and improvement of diagnostic capabilities to elucidate the current disease burden of leptospirosis. [4][5][6][7][8][9][10][11][12][13] However, there are limited studies on the in vitro and in vivo screening to find alternative treatments (i.e. use of herbal plants) for leptospirosis.…”

Section: Introductionmentioning

confidence: 99%

Optimized Tube Dilution Technique and Sole Carbon Utilization Assay for Anti-leptospiral In Vitro Screening of Plant Extracts

Manglicmot-Yabes¹,

Villanueva²,

Gloriani³

2020

PIDSPJ

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Introduction: Leptospirosis is one of the neglected reemerging zoonoses that is of public health concern globally. The need to discover novel therapeutic alternatives for leptospirosis through screening for and elucidating the mechanism/s of the anti-leptospiral activity of plant extracts is therefore necessary. This study analyzes the optimized tube dilution technique and the BiologTM sole carbon utilization phenotype microarray as screening tool for anti-leptospiral activity of plant extracts. Methods: The suitability of the optimized tube dilution technique was evaluated by determining the minimum inhibitory concentration (MIC), minimum bactericidal concentration (MBC), and motility inhibition property of a plant extract and an antimicrobial control (pen G) against 4 dominantly circulating Leptospira serovars/serogroup in the Philippines. Likewise, the suitability of the BiologTM sole carbon utilization assay was evaluated using a plant extract and selected antimicrobials against L. interrogans serovar Manilae strain K64 and L. interrogans serovar Losbanos strain K37. Results: The MIC, MBC, and motility inhibition property of a plant extract and the antibiotic controls as well as its effect on the carbon utilization phenome of the Leptospira serovars gave consistent results, within and between several runs. With standard deviation = 0 for all serovars. The MIC and MBC of the antimicrobial control (pen G), the positive control, was 10 ug/ml. The growth control (leptospires without treatment), the negative control, showed presence of motile leptospires. The MIC and the MBC of the test plant extract was 250 ug/ml – 500 ug/ml. Results of the carbon utilization phenome or pattern of carbon utilization were consistent within the 3 replicates and between two runs. Conclusion: The optimized tube dilution technique and the BiologTM sole carbon utilization assay is a potential in vitro screening tool for determining anti-leptospiral activity of plant extracts.

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Carbon utilization phenome of Leptospira interrogans serovar Manilae strain K64

Manglicmot-Yabes

Villanueva

Gloriani

2021

LSMB

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Background: Leptospirosis, an acute febrile disease caused by the pathogenic species of genus Leptospira, is one of the neglected emerging zoonoses that is of global public health concern. The recent genus-wide sequencing of Leptospira isolates led to the need for better understanding of the complex metabolic mechanisms of this organism. However, majority of the published studies on Leptospira metabolism were still the pioneering works of Baseman and Cox in the 60’s and their contemporaries. Knowledge on the carbon sources that supports the growth of a Leptospira species will not only contribute to the limited metabolic studies but will further support the reported genes and metabolic pathways of this organism. Objective: Thus, this study aimed to describe the carbon utilization phenome of Leptospira interrogans serovar Manilae strain K64, one of the dominantly circulating pathogenic Leptospira in the Philippines. Methods: A previously optimized BiologTM Gen III sole carbon utilization phenotype microarray assay protocol for leptospires was adapted. Results: L. interrogans serovar Manilae strain K64 showed utilization of 29 carbon sources belonging to sugars and sugar derivatives, amino acids, methyl ester, carboxylic acid and fatty acids. These were N-acetyl-β-D-mannosamine, N-acetyl-D-galactosamine, N-acetyl neuraminic acid, D-fructose, D-galactose, 3-methyl glucose, D-fucose, L-fucose, L-rhamnose, inosine, D-fructose-6-phosphate, D-gluconic, D-glucuronic acid, glucuronamide, D-saccharic acid, D-aspartic acid, D-serine, L-alanine, L-arginine, L-histidine, L-pyroglutamic acid, L-serine, D-lactic acid methyl ester, citric acid, D-malic acid, L-malic acid, alpha ketoglutaric acid, alpha ketobutyric acid, and acetoacetic acid. Discussion and Conclusion: The carbon sources utilized by L. interrogans serovar Manilae strain K64 agreed well with the identified genes and metabolic pathways among Leptospira species. Moreover, these 29 carbon sources have been previously reported to be associated in the biosynthesis of peptidoglycan, lipopolysaccharide, histidine, sulfur, amino acids, and isoleucine and in other metabolic pathways such as glycolysis, pentose-phosphate, pyruvate and fatty acid in Leptospira spp.

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Seroprevalence of Leptospirosis among Water Buffaloes, Pigs, and dogs in Selected Areas in the Philippines, 2007 to 2008

Cited by 3 publications

References 0 publications

Canine leptospirosis in stray and sheltered dogs: a systematic review

Canine leptospirosis in stray and sheltered dogs: a systematic review

Optimized Tube Dilution Technique and Sole Carbon Utilization Assay for Anti-leptospiral In Vitro Screening of Plant Extracts

Carbon utilization phenome of Leptospira interrogans serovar Manilae strain K64

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