Seroprevalence of Mycoplasma gallisepticum in Commercial Poultry Birds Showing Respiratory Clinical Signs in Chakwal District, Pakistan

Haider, Shujjah; Maqbool, Ayesha; Pervez, Tariq; Parveen, Saima; Ahmad, Arfan; Iqbal, Zahid; Iqbal, Javed; Mehmood, Shahid; Khan, Aman Ullah; Umar, Sajid

doi:10.17582/journal.pjz/2019.51.5.sc7

Cited by 2 publications

(3 citation statements)

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“…Meanwhile, in RSA test, 99 were positive (62%) and just 56 were negative (38%). ELISA test result in this study show better sensitivity and specivicity than RSA, was similar to previous studies [6,7]. Other studies identified ELISA as a specific, easy and effective assay.…”

Section: Resultssupporting

confidence: 90%

“…The result in this study appears to be consistent with the ability of the RSA test to detect Ig M early in infection from MG. The other studies state that in detecting IgM antibodies 7-10 days after MG infection, RSA is highly effective [2,7]. The lack of sensitivity of ELISA in detecting MG infection at the age of 28-32 in this study may be associated with initial infection.…”

Section: Resultsmentioning

confidence: 67%

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Comparing the suitability of the serologic tests in diagnose Mycoplasma gallisepticum

Purba,

Rachmawati,

Purwanto

et al. 2023

IOP Conf. Ser.: Earth Environ. Sci.

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Mycoplasma gallisepticum (MG) infection in chickens results in significant economic losses because it reduces egg production. Chickens infected with MG are stated to have a higher susceptibility to other disease agents. Therefore it is vital to monitor the presence of MG in layer farms. Monitoring through serological tests is the easiest option to apply. Rapid Agglutination Serum (RSA) is an option because of its fast and inexpensive application. In addition, ELISA has also been used to confirm the RSA test. This study compares the suitability of two serological tests, the RSA and ELISA. Serum samples of 155 taken from laying hens were tested by RSA and ELISA. RSA test results obtained 99 (63.9%) seropositive and 105 (67.7%) ELISA seropositive. As many as 68 (64.8%) were seropositive on the RSA and ELISA tests, while 19 (38%) were seronegative. The proportion of conformity by the Mc Nemar test between the RSA and ELISA tests did not show a significant difference of 0.545 (P>0.005). The kappa value between the two serological techniques was low (k=0.027). The statistical analysis results of these two serological tests show that these two tests can be used as initial monitoring for screening for MG.

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Section: Resultssupporting

confidence: 90%

Section: Resultsmentioning

confidence: 67%

Comparing the suitability of the serologic tests in diagnose Mycoplasma gallisepticum

Purba,

Rachmawati,

Purwanto

et al. 2023

IOP Conf. Ser.: Earth Environ. Sci.

View full text Add to dashboard Cite

show abstract

“…In Pakistan an overall prevalence of Mycoplasmosis was detected 29.88% and 20. 67% using ELISA and SPA test respectively (Haider et al, 2019). But the prevalence of mycoplasnosis in present study showed a bit higher than the previously published research (Rahman et al, 2016;Haque et al, 2015;Abdullah et al, 2019;Uddin et al, 2010;Talha et al, 2001) and lower than other some studies (Hossain et al, 2007;Sikder et al, 2005;Barua et al, 2006;Hossain et al, 2010).…”

Section: Overall Prevalence Of Mycoplasmosis In Commercial Poultrycontrasting

confidence: 67%

Macroscopic and microscopic characterization of mycoplasmosis in commercial chickens in Barishal, Bangladesh

Bepary

Rahman

et al. 2022

Asian Australas. J. Biosci. Biotechnol.

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Avian mycoplasmosis in commercial chicken is a serious problem around the world, and responsible for high economic losses and decreased poultry productivity. This study aimed to characterize the mycoplasma infection macroscopically and microscopically in commercially important poultry like broiler and layer in Barishal, Bangladesh. Diagnosis of mycoplasmosis was made on the basis of history, clinical signs, necropsy findings and histopathology of the suspected organs. The study was conducted on a total no. of 460 birds (broiler n=237; layer n=223) of 20 different farms of three different upazillas of Barishal district in Bangladesh. The overall prevalence of micoplasmosis was 26.52%. Layer chicken (30.04%) was more susceptible to mycoplasmosis than broiler chicken (23.20%). The highest prevalence of mycoplasmosis was observed in 3-6 weeks-aged chicken in both broiler (54.55%) and layer (35.82%) chicken. A significant difference (p<0.05) was observed in mycoplasmosis prevalence rate among the seasons. The highest prevalence rate of mycoplasmosis was in winter season followed by rainy and seasons. Major necropsy findings of mycoplasmosis include catarrhal hemorrhage and mucus exudation on tracheal mucosa and severe congestion, consolidation and reddish gelatinous mass over the lung surface. Mycoplasmosis caused severe tracheitis, bronchitis, air sacculitis, and pneumonia in chickens. Mycoplasma affected different organs in the chicken and hinder the production through morbidity and mortality. Present study will provide the baseline data for future in-depth research on mycoplasmosis to draw an effective policy for controlling and eradicating mycoplasmosis from commercial poultry. Asian Australas. J. Biosci. Biotechnol. 2021, 6 (3), 153-160

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Seroprevalence of Mycoplasma gallisepticum in Commercial Poultry Birds Showing Respiratory Clinical Signs in Chakwal District, Pakistan

Cited by 2 publications

References 0 publications

Comparing the suitability of the serologic tests in diagnose Mycoplasma gallisepticum

Comparing the suitability of the serologic tests in diagnose Mycoplasma gallisepticum

Macroscopic and microscopic characterization of mycoplasmosis in commercial chickens in Barishal, Bangladesh

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