Seroprevalence of TORCH Infections in Children

Biswas, Subhasis B.; Badruddoza,; Sultana, Nahid

doi:10.3329/cmoshmcj.v17i1.39436

Cited by 3 publications

(11 citation statements)

References 8 publications

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“…Total IgM (±IgG) positivity rate (26%) in this study may seem slightly more than the previous related studies from India (Mahalakshmi B-20.2%, Singh MP-15.8%, Shyamala-6.0%) and neighboring countries (Bin Lu-18.84%, Saleem T-23.5%, Sharma-0.0%, Biswas SK-43.1%). [ 1 7 8 18 19 20 21 ] We presume this to be due to the marginal higher sensitivity of the EUROIMMUN kit that we used, as compared to the other validated ELISA kit. [ 23 24 ] We believe this issue was neutralized at least to some extent, by performing quantitative CLIA simultaneously.…”

Section: Discussionmentioning

confidence: 99%

“…However, this cannot be compared with the currently available relevant studies in India or neighboring countries, primarily because of differences in methodology, inclusion criteria of age, and adoption of the entire ToRCH-profile. [ 1 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 ]…”

Section: Discussionmentioning

confidence: 99%

“…Ironically, there were no IgM-positive cases reported from our neighboring countries, except Bangladesh (5.17%). [ 1 7 18 19 20 21 ]…”

Section: Discussionmentioning

confidence: 99%

“…[ 1 7 8 9 10 11 12 13 14 15 16 17 ] However, corresponding figures reported from neighboring countries such as Nepal (0.0%), Pakistan (0.0%), and China (1.4%) were quite surprising. [ 18 19 20 21 ]…”

Section: Discussionmentioning

confidence: 99%

“…A few reported studies involved subjects <1 year of age, considered one or few selective pathogens, made a diagnosis based only on a single qualitative serum test or lacked a clearly defined significance of IgG/IgM. [ 1 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 ] This study was thus conducted to determine, analyze and report the ToRCH-serology screening profile among pediatric cataract cases.…”

mentioning

confidence: 99%

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ToRCH-screening in pediatric cataract revisited: A North Indian tertiary care centre study

Dasgupta¹,

Shakeel²,

Roy³

2020

Indian J Ophthalmol

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Purpose: To analyze and report ToRCH-serology screening profile ( Toxoplasma gondii [TOX], rubella [RV], cytomegalovirus [CMV], and herpes simplex virus [HSV-I/II]) in pediatric cataract. Methods: In this prospective analytical study, 1,026 consecutive children were screened, of which 46 children with clinically diagnosed congenital ( n = 26) and developmental cataract ( n = 20) were included. Post-traumatic and familial cataracts were excluded. Sera of all children were tested both qualitatively and quantitatively for IgG/IgM-antibodies against ToRCH agents in a sequential manner. Results: Overall, IgM/IgG-seropositivity against ≥1 ToRCH agent was reported in 91.3% (42/46) children. IgM (±IgG) positivity against ≥1 ToRCH agent was reported in 26.08% (12/46) children (nine congenital and three developmental cataract; P = 0.18), which included 8.7% (4/46) children reported positive against ≥2 agents. Finally, 13% (6/46) children were reported to be sero-clinical-positive (three were infants and three were >1 year age, P = 0.55; five congenital and one developmental cataract, P = 0.21). Either alone or combined, RV attributed to the majority (50%; 6/12) of the IgM (±IgG) and sero-clinical-positive (50%; 3/6) children. None of the children were HSV-II IgM-positive. Laboratory-confirmed congenital rubella syndrome was reported in 4.3% (2/46) children. One sero-clinical-positive infant with rare coexisting bilateral persistent fetal vasculature was also reported. IgG-alone positivity was reported highest with CMV in 67.4% (31/46) children, whereas 43.4% (20/46) children were found nonimmune to RV. Conclusion: The current study emphasizes the need to interpret ToRCH-screening in pediatric cataract with caution. Interpretation should include both serial qualitative and quantitative assays in tandem with clinical correlation to minimize the diagnostic errors. Clinicians should remain vigilant regarding sero-clinical-positivity in older children too who might pose a threat to the spread of infection.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

“…Ironically, there were no IgM-positive cases reported from our neighboring countries, except Bangladesh (5.17%). [ 1 7 18 19 20 21 ]…”

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

mentioning

confidence: 99%

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ToRCH-screening in pediatric cataract revisited: A North Indian tertiary care centre study

Dasgupta¹,

Shakeel²,

Roy³

2020

Indian J Ophthalmol

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Advanced Laboratory Investigations Conducted at Medical Research Unit Laboratory, MHWT, Uttara Model Town, Dhaka, Bangladesh

Giasuddin¹

2020

AJBSR

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Since the establishment of radioimmunoassay (RIA), enzyme immunoassay (EIA), discovery of monoclonal antibody (MCA) and fluorescence activated cell sorter (FACS), tremendous progress have been made in the field of diagnostic immunology. MCA based advanced techniques such as RIA, EIA, FACS, nephelometry, immunofluorescence technique, immunochromatographic technique (ICT), etc are presently used in modern diagnostic immunology. With the application of these powerful techniques, laboratory diagnosis and follow-up of immunologically-mediated as well as other diseases are made now-a-days with high precision and accuracy [1-6]. Immunology laboratory were reorganized and more versatile tests were introduced at the Medical College for Women & Hospital (MCW&H) in August 2002. The idea was to provide high quality laboratory service for specialized diagnostic immunology tests in a cost effective way. In 2005 Medical Research Unit (MRU) had been set up in at MCW&H building to conduct high quality clinical and biomedical research and provide further specialized biochemical and immunological laboratory tests at affordable costs. Many diagnostic tests were introduced and carried out regularly at MRU (Diagnostic) Laboratory to confirm and pinpoint the clinical (provisional) diagnosis of human diseases. The analysis of the results of some advanced laboratory investigations

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Identification of Torch Pathogens in Congenital Cataract Patients Visiting Armed Forces Institute of Ophthalmology

Javaid

Masud

Rathore

et al. 2021

PAFMJ

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Objective: To assess the rate of positivity of Toxoplasma gondii, Rubella, Cytomegalovirus, Herpes simplex (TORCH) pathogens in patients suffering from congenital cataract in Pakistan. Study Design: Cross sectional comparative study. Place and Duration of Study: Armed Forces Institute of Ophthalmology, Rawalpindi, from Jun 2017 to Mar 2018. Methodology: Cataract group included sixty children already diagnosed with congenital cataract and control group included 60 children not suffering from congenital cataract. Serum samples of all children were collected to test against IgG and IgM antibodies of Toxoplasma gondii, Rubella, Cytomegalo, Herpes simplex pathogens using enzyme linked immune essay (ELISA) technique. Data were analyzed observe in difference between unilateral and bilateral cataract and between cataract and control group. Results: Out of 60 children in cataract group, 29 were tested positive for Toxoplasma gondii, Rubella, Cytomegalo (CMV), Herpes simplex infections. CMV IgG antibodies were identified in 21 (35%) children followed by Rubella IgG 13 (21.7%), Toxoplasmosis IgG 8 (13.3%), CMV IgM 7 (11.7%), HSV I IgG 5 (8.3%), HSV II IgG 2 (3.3%) and HSV I IgM 1 (1.7%) antibodies. None of the children was positive for Toxoplasmosis, Rubella and HSV II IgM antibodies. There was no statistical difference in rate of positivity of Toxoplasma gondii, Rubella, Cytomegalovirus, Herpes simplex pathogens between unilateral and bilateral cataract and between cataract and control group. Conclusion: Toxoplasma gondii, Rubella, Cytomegalovirus, Herpes simplex pathogens are an important cause of congenital cataract with Cytomegalovirus and Rubella virus being most common respectively. In order to prevent paediatric complications, we need........

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Seroprevalence of TORCH Infections in Children

Cited by 3 publications

References 8 publications

ToRCH-screening in pediatric cataract revisited: A North Indian tertiary care centre study

ToRCH-screening in pediatric cataract revisited: A North Indian tertiary care centre study

Advanced Laboratory Investigations Conducted at Medical Research Unit Laboratory, MHWT, Uttara Model Town, Dhaka, Bangladesh

Identification of Torch Pathogens in Congenital Cataract Patients Visiting Armed Forces Institute of Ophthalmology

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