Seroprevalence Studies of Brucellosis among Human using Different Serological Tests

Sharma, Vijay Prakash; Sharma, Harsh; Ganguly, Subha; Berian, Sindhu; Malik, Mohammad Bilal

doi:10.20546/ijcmas.2017.605.195

Cited by 6 publications

(7 citation statements)

References 9 publications

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“…Among serological tests, the highest seropositivity was recorded by iELISA (17.13%) than RBPT (13.67%). Similar results have been reported [ 23 - 26 ]. In contrast, few authors have reported lower seropositivity by iELISA compared to the RBPT [ 17 , 23 , 27 ].…”

Section: Discussionsupporting

confidence: 93%

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Seroprevalence of brucellosis in small ruminants in organized and unorganized sectors of Gujarat state, India

Kanani¹,

Dabhi²,

Patel³

et al. 2018

Vet World

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Aim:The present study aimed to study the seroprevalence of brucellosis in small ruminants of Gujarat state, India, using Rose Bengal Plate test (RBPT) and indirect enzyme-linked immunosorbent assay (iELISA).Materials and Methods:A total of 2444 sera samples (675 sheep and 1769 goat) from unorganized sector and 1310 sera samples (861 sheep and 449 goat) from seven organized farms were collected for brucellosis screening.Results:In unorganized sector, 23.70% sheep (160/675) and 15.99% goat (283/1769) were positive by RBPT and 24.44% sheep (165/675) and 17.24% goat (305/1769) by iELISA. The organized sector samples showed higher seroprevalence in goat (7.79 %, 35/449) than sheep (4.06 %, 35/861) by RBPT. Similarly, in iELISA, goat samples showed a higher seroprevalence (9.35%, 42/449) compared to sheep (7.50%, 65/861). The diagnostic sensitivity and specificity of RBPT with ELISA were 88.69% and 99.65%, respectively, and showed a significant difference (p≤0.0001). The Chi-square analysis revealed a significant difference in seroprevalence between sectors (p≤0.01) and species (p≤0.01).Conclusion:The seroprevalence of brucellosis in small ruminants of Gujarat was investigated and showed a higher prevalence of brucellosis and warrants the implementation of proper preventive measures.

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Section: Discussionsupporting

confidence: 93%

“…The results were similar to the Sharma et al . [ 26 ] and contrary to few reports [ 25 , 28 , 29 ]. The low prevalence of brucellosis in the organized sector could be due to better management practices and routine screening of animals for brucellosis.…”

Section: Discussionmentioning

confidence: 82%

Seroprevalence of brucellosis in small ruminants in organized and unorganized sectors of Gujarat state, India

Kanani¹,

Dabhi²,

Patel³

et al. 2018

Vet World

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“…In recent studies conducted in small ruminants elsewhere a considerable number of sera which were positive by mRBPT were found negative by ELISA [34][35][36]. In this study, a high seroprevalence for Brucella infection was detected in those animals with retained placenta which is similar to the results of previous studies elsewher [37][38][39][40]. A review on clinical features of brucellosis also showed that placenta retention is one of the main clinical signs of B. melitensis infection in aborted small ruminants [1].…”

Section: Discussionsupporting

confidence: 88%

Seropositivity and risk factors of Brucella infection in small ruminants that had history of recent abortion in the afar region of north-eastern Ethiopia

Tekle

Legesse

Ameni

et al. 2020

Preprint

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Background: Brucellosis is one of the most important reproductive disease causes abortion and breeding failure in small ruminants and also causes severe systemic diseases in exposed humans. In Ethiopia, several studies of seroprevalence shows the magnitude and distribution of brucellosis both in animals and humans vary in different geographical localities. However, except few studies in Ethiopia all these serological studies was limited to RBPT and CFT, so far not supplemented with a varities of serological tests like ELISA to detect brucella infection, which is increase the likehood of detecting infected individulas and also improve the reliability of epidemiological data for appropriate control strategies. Hence, the present study was conducted in Amibara district of Afar Region, Ethiopia to detect the seropositivity and risk factor of Brucella infection in small ruminants that had history of recent abortion using mRBPT, cELISA and CFT. Materials and methods: Sera were collected from 226 animals (195 goats and 31sheep) and assessed for seropositivity of Brucella infection using modified Rose Bengal Plate Test (mRBPT), Complement Fixation Test (CFT) and competitive Enzyme Linked Immuno Sorbent Assay (cELISA).Results : In this study the over all seroprevalence was 12.0% (27 out of 226), 7.5% (17 out of 226) and 26.6% (60 out of 226) by mRBPT, CFT and cELISA, respectively. Out of 27 sera which were reactive by mRBPT, 17 (63.0%) were also reactive by (CFT). Out of the 17 sera which were reactive by CFT and mRBPT, 14 (82.4%) were reactive by cELISA. Out of the 29 sera which were non-reactive both by mRBPT and CFT, 10 (34.5%) were found to be reactive by cELISA. Out of the 226 sera which were tested both by mRBPT and cELISA, 20 (8.9%) were reactive by both tests, while 159 (70.4%) were non-reactive by both tests. The percentage of test agreement (79.2%) between mRBPT and cELISA was poor (k= 0.353). A high seropositivity for Brucella infection was significantly associated with the presence of retained placenta in the study animals (adjusted OR= 2.2, 95%CI, 1.1-4.4, P=0.030) as detected by cELISA. Conclusion: The findings of this study could suggest that brucellosis is main cause of abortion and breeding failure in small ruminants that had histry of recent abortion in the pastoral communities’ andwarrants the need for proactive measures to reduce its economic impact and risk of zoonotic transmission. This study indicates that cELISA based seroepidemiological survey increase the likehood of detecting infected individulas of brucellosis and also would be useful to provide reliable evidence for Brucella infection in small ruminants compared to mRBPT.

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“…While the seroprevalence by STAT in goats in the present study falls in similar range to a study in Jammu, India i.e. 3.42 % (31), it was below other reports from various parts of India which showed seropositivity ranging from 7.96 per cent to 17.68 per cent (11,32,33). The seroprevalence was higher in female animals (4.00%) which may be due to higher stocking of females compared to males, as the male animals are regularly sent for slaughter (for meat).…”

Section: Results and Dicsussionsupporting

confidence: 52%

Serological and Molecular Study on Caprine Brucellosis in Puducherry (India) and its Public Health Significance

Madan¹,

Kumaresan

Rekha³

et al. 2022

Preprint

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Caprine brucellosis due to Brucella melitensis is an important zoonotic disease. The present study was carried out to address the lack of a comprehensive study on the status of caprine brucellosis in Puducherry, India using serological and molecular tests in goats and to assess the seroprevalence in human risk groups of the aforementioned region to ascertain the public health significance of the disease. Seroprevalence in 120 goats was found to be zero, 3.33% and 18.33% by Rose Bengal agglutination Test (RBT), Standard Tube Agglutination Test (STAT) and Immunoglobulin G Indirect Enzyme Linked Immune Sorbant Assay (IgG iELISA) respectively. Of the 120 goat genital swabs screened, while conventional polymerase chain reaction (PCR) detected genus specific 16S rRNA and Brucella melitensis specific omp2 genes in 17.50% and 5.00% of samples respectively, the OMP31TaqMan® real time PCR with a positive detection of 40.00% was both the most sensitive and specific for detection of Brucella melitensis. The study provides insight into the optimization of diagnostic tests following cluster wise sampling for brucellosis in goats. The strain of B. melitensis in Puducherry was found to be Biovar 3 based upon suggestive results of Restriction Fragment Length Polymorphism (RFLP) of omp2 gene product. Seroprevalence by IgG iELISA was 33.33 % in 30 samples from human subjects. Serological evidence of caprine brucellosis in goats and human subjects and molecular detection of B.melitensis in Puducherry, India warrants regular screening, surveillance and reporting of disease in goats and human risk groups.

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Seroprevalence Studies of Brucellosis among Human using Different Serological Tests

Cited by 6 publications

References 9 publications

Seroprevalence of brucellosis in small ruminants in organized and unorganized sectors of Gujarat state, India

Seroprevalence of brucellosis in small ruminants in organized and unorganized sectors of Gujarat state, India

Seropositivity and risk factors of Brucella infection in small ruminants that had history of recent abortion in the afar region of north-eastern Ethiopia

Serological and Molecular Study on Caprine Brucellosis in Puducherry (India) and its Public Health Significance

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