Seroprevalence to Trypanosoma cruzi in rural communities of the state of Querétaro (Mexico)

Villagrán, M. E.; Sánchez-Moreno, M.; Marı́n, Clotilde; Uribe, Mario Alexander Melo; Cruz, Javier; Diego, J. A. de

doi:10.1016/j.clinbiochem.2008.09.122

Cited by 10 publications

(11 citation statements)

References 12 publications

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“…More recently, however, it has been estimated that there are as many as two million infected individuals and more than 72 000 cases in Mexico and Central America [2]. Even though these figures are only estimates, other field studies support the notion that the prevalence of T. cruzi infection might be higher in some regions of the country than previously thought [3–5]. T. cruzi has also been repeatedly found infecting insects and mammals in Mexico and the United States of America [6–8].…”

Section: Introductionmentioning

confidence: 99%

MexicanTrypanosoma cruzi(TCI) Strains with Different Degrees of Virulence Induce Diverse Humoral and Cellular Immune Responses in a Murine Experimental Infection Model

Espinoza

Rico

Sosa

et al. 2010

Journal of Biomedicine and Biotechnology

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It is has been shown that the majority of T. cruzi strains isolated from Mexico belong to the T. cruzi I (TCI). The immune response produced in response to Mexican T. cruzi I strains has not been well characterized. In this study, two Mexican T. cruzi I strains were used to infect Balb/c mice. The Queretaro (TBAR/MX/0000/Queretaro)(Qro) strain resulted in 100% mortality. In contrast, no mortality was observed in mice infected with the Ninoa (MHOM/MX/1994/Ninoa) strain. Both strains produced extended lymphocyte infiltrates in cardiac tissue. Ninoa infection induced a diverse humoral response with a higher variety of immunoglobulin isotypes than were found in Qro-infected mice. Also, a stronger inflammatory TH1 response, represented by IL-12p40, IFNγ, RANTES, MIG, MIP-1β, and MCP-1 production was observed in Qro-infected mice when compared with Ninoa-infected mice. We propose that an exacerbated TH1 immune response is a likely cause of pathological damage observed in cardiac tissue and the primary cause of death in Qro-infected mice.

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Section: Introductionmentioning

confidence: 99%

MexicanTrypanosoma cruzi(TCI) Strains with Different Degrees of Virulence Induce Diverse Humoral and Cellular Immune Responses in a Murine Experimental Infection Model

Espinoza

Rico

Sosa

et al. 2010

Journal of Biomedicine and Biotechnology

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“…In addition, studies made with parasites belonging to the family Trypanosomatidae have corroborated the presence of this Fe-SODe [17, 22]. The differences between the protozoan Fe-SODe and those of the hosts, together with the immunogenic properties, make this Fe-SODe useful as a feasible molecular marker for the development of new diagnostic methods, in addition to potential therapeutic targets for designing new anti-Chagas drugs with the potential of taking advantage of the structural differences between Fe-SOD protozoans and the copper-zinc-SOD of most eukaryotes [22].…”

Section: Resultsmentioning

confidence: 93%

“…This is a serious drawback in patients from geographical areas harbouring other infectious agents. However, in previous works, we have demonstrated that Fe-SODe is species specific and thus does not present cross reactions with other trypanosomatids [12, 14, 17]. …”

Section: Resultsmentioning

confidence: 94%

“…The prevalence reported is extremely varied, the data going from 2.8% for the state of Nuevo León, to 16.8% reported for the state of Veracruz [25, 26]. In the state of Querétaro, the prevalence of this disease is unknown and data are available only for the year 2005 [17], when a prevalence of 8.6% was found in the rural population. For this reason, the second objective of the present study was to provide new epidemiological data on the disease in this state of Mexico, focusing on the suburban areas of the capital of this state.…”

Section: Resultsmentioning

confidence: 99%

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Trypanosoma cruzi: Seroprevalence Detection in Suburban Population of Santiago de Querétaro (Mexico)

López-Céspedes

Villagrán

Álvarez

et al. 2012

The Scientific World Journal

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Objectives. To evaluate the potential of iron-oxide dismutase excreted (SODeCRU) by T. cruzi as the antigen fraction in the serodiagnosis of Chagas disease and compile new epidemiological data on the seroprevalence of this disease in the suburban population of the city of Santiago de Querétaro (Mexico). Design and Methods. 258 human sera were analyzed by the techniques of ELISA and Western blot and using the homogenate and the SODeCRU. Results. A total of 31 sera were positive against ELISA/SODeCRU (12.4%), while 30 sera proved positive by WB/SODeCRU (11.6%). The comparison between the technique of ELISA and WB showed a sensitivity of 93%, and a specificity of 99%. The positive predictive value was 93% and the negative predictive value was 99%, with a Kappa (κ) value of 1. Conclusions. These preliminary data reveal the degree of infection of nonrural areas of Mexico and demonstrated that SODeCRU is an antigen useful to diagnose Chagas disease.

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“…Evaluations of statistically significant differences between the tests were based on sensitivity, specificity, negative and positive predictive values, concordance percentage and the kappa index. The following epidemiological variables were considered in this study: presence of the vectors Triatoma mexicana (94%), Triatoma dimidiate (3%), Triatoma gerstaeckeri (2%) and Triatoma pallidipennis (1%) ( Villagrán et al 2009) , gender, age, housing type, co-habitation with animals, trips outside the community and reported symptomatology that was compatible with known signs and symptoms of the acute or chronic phases of Chagas disease. Comparisons between epidemiologic parameters and survival patterns were established in every studied population ( Ferrer et al 2013) .…”

Section: Subjects Materials and Methodsmentioning

confidence: 99%

Comparative serology techniques for the diagnosis of Trypanosoma cruzi infection in a rural population from the state of Querétaro, Mexico

Villagrán-Herrera

Sánchez‐Moreno

Rodríguez-Méndez

et al. 2014

Mem. Inst. Oswaldo Cruz

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Immunological diagnostic methods for Trypanosoma cruzi depend specifically on the presence of antibodies and parasitological methods lack sensitivity during the chronic and “indeterminate” stages of the disease. This study performed a serological survey of 1,033 subjects from 52 rural communities in 12 of the 18 municipalities in the state of Querétaro, Mexico. We detected anti-T. cruzi antibodies using the following tests: indirect haemagglutination assay (IHA), indirect immunofluorescence assay (IFA), ELISA and recombinant ELISA (rELISA). We also performed Western blot (WB) analysis using iron superoxide dismutase (FeSOD), a detoxifying enzyme excreted by the parasite, as the antigen. Positive test results were distributed as follows: ELISA 8%, rELISA 6.2%, IFA and IHA 5.4% in both cases and FeSOD 8%. A comparative study of the five tests was undertaken. Sensitivity levels, specificity, positive and negative predictive values, concordance percentage and kappa index were considered. Living with animals, trips to other communities, gender, age, type of housing and symptomatology at the time of the survey were statistically analysed using SPSS software v.11.5. Detection of the FeSOD enzyme that was secreted by the parasite and used as an antigenic fraction in WBs showed a 100% correlation with traditional ELISA tests.

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Seroprevalence to Trypanosoma cruzi in rural communities of the state of Querétaro (Mexico)

Cited by 10 publications

References 12 publications

MexicanTrypanosoma cruzi(TCI) Strains with Different Degrees of Virulence Induce Diverse Humoral and Cellular Immune Responses in a Murine Experimental Infection Model

MexicanTrypanosoma cruzi(TCI) Strains with Different Degrees of Virulence Induce Diverse Humoral and Cellular Immune Responses in a Murine Experimental Infection Model

Trypanosoma cruzi: Seroprevalence Detection in Suburban Population of Santiago de Querétaro (Mexico)

Comparative serology techniques for the diagnosis of Trypanosoma cruzi infection in a rural population from the state of Querétaro, Mexico

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