SEROPREVALENCIA DE Neospora caninum EN BOVINOS LECHEROS DE CUATRO DISTRITOS DEL VALLE DEL MANTARO, JUNÍN.

Granados, Z Silvia; Rivera, G Hermelinda; Casas, A Eva; Suárez, A Francisco; Arana, D Carlos; Chávez, V Amanda

doi:10.15381/rivep.v25i1.8468

Cited by 7 publications

(5 citation statements)

References 11 publications

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“…Seroprevalence of N. caninum in dairy cattle has been reported in different regions of Peru and described their association with aborts.. In the highlands, a seroprevalence of 46.7% was reported (GRANADOS et al, 2014), while in the Amazon rainforest it was 18.8% (PORTOCARRERO et al, 2015), in Lima Valley (Peru central coast) it was 29.6%-69.6% (GAMARRA et al, 2009;SILVA et al, 2002), and in our study it was 46.6%. Rivera reported 55% of aborted fetus with N. caninum presence in differents farms from Lima valley with different diagnostic test (RIVERA, 2001) and our study found 15% of aborted fetus.…”

Section: Discussionsupporting

confidence: 54%

Evaluation of abortions spontaneously induced by Neospora caninum and risk factors in dairy cattle from Lima, Peru

Serrano‐Martínez

Cisterna

Romero

et al. 2019

Rev. Bras. Parasitol. Vet.

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Our objective was to identify the direct and indirect presence of Neospora caninum in dairy cattle and their aborted fetuses from Lima, Peru. A total 219 blood samples obtained from dairy cattle with records of spontaneous abortion were collected to detect antibodies against N. caninum in serum with indirect ELISA and search for risk-factor associations. 68 fetal aborted tissue samples of these cows were analyzed by PCR, indirect ELISA and histopathology assay to detect N. caninum presence. The prevalence ratio (PR) and 95% confidence intervals (CI) were estimated. Univariate analysis was performed using the chi-squared test. Among the 68 aborted fetuses collected, 10 (15%) were positive in at least two diagnostic tests. Among 219 serum samples, 46.6% (95% CI: 40.0%-53.3%) were positive. Cows with 4 years or older (PR: 7.10; 95% CI: 4.89-10.67) and multiparous (PR: 1.76; 95% CI: 1.11-2.80) were found to be more likely to possess N. caninum antibodies. This study detects presence of N. caninum in dairy cattle and their aborted fetus from Lima valley, suggesting biosecurity management improve to neosporosis control.

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Section: Discussionsupporting

confidence: 54%

Evaluation of abortions spontaneously induced by Neospora caninum and risk factors in dairy cattle from Lima, Peru

Serrano‐Martínez

Cisterna

Romero

et al. 2019

Rev. Bras. Parasitol. Vet.

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“…Thus, taking these parameters into account, in this work a logistic regression analysis was carried out, which gave us a result of species vs. primer association which was selective and specific with the numerical function and thus useful to discriminate between species of the same genus. Furthermore, the database was validated with the statistical program JMP ® Pro 13 and was built through visual analysis without using programs such as GelCompar II [ 35 ], PyElph [ 36 ], Quantity One [ 37 ] and Image Master [ 38 ], among others, which minimize the bias generated when obtaining data from polymorphic patterns; the data were also not normalized, as recommended in the literature [ 39 ]. The logistic regression model allowed the obtaining of ROC curves, which evaluated the sensitivity against the specificity of each primer for the different Aspergillus spp.…”

Section: Discussionmentioning

confidence: 99%

Selection of Polymorphic Patterns Obtained by RAPD-PCR through Qualitative and Quantitative Analyses to Differentiate Aspergillus fumigatus

Ledezma

Castro-Fuentes

Duarte-Escalante

et al. 2022

JoF

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The objective of this work was to use the random amplification of the polymorphic DNA–polymerase chain reaction (RAPD-PCR) technique to select polymorphic patterns through qualitative and quantitative analyses to differentiate the species A. flavus, A. fumigatus, A. niger and A. tubingensis. Twenty-seven Aspergillus isolates from different species were typified using phenotypic (macro- and micromorphology) and genotypic (partial BenA gene sequencing) methods. Thirty-four primers were used to obtain polymorphic patterns, and with these a qualitative analysis was performed to select the primers that presented species-specific patterns to distinguish each species. For the quantitative selection, a database was built from the polymorphic patterns and used for the construction of logistic regression models; later, the model that presented the highest value of sensitivity against specificity was evaluated through ROC curves. The qualitative selection showed that the primers OPA-19, P54, 1253 and OPA-02 could differentiate the species. A quantitative analysis was carried out through logistic regression, whereby a species-specific correlation of sensitivity and specificity greater than 90% was obtained for the primers: OPC-06 with a 96.32% match to A. flavus; OPF-01 with a 100% match to A. fumigatus; OPG-13 with a 98.01% match to A. tubingensis; and OPF-07 with a 99.71% match to A. niger. The primer OPF-01 discriminated the four species as well as closely related species. The quantitative methods using the selected primers allowed discrimination between species and showed their usefulness for genotyping some of the species of medical relevance belonging to the genus Aspergillus.

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“…Estos datos los cuales fueron muestreados 87 animales concuerdan con otros estudios epidemiológicos pertenecientes a los Distritos de San Lorenzo, realizados sobre esta infección para otras zonas. Ñemby y Nueva Italia, del Departamento Central, con el objetivo de conocer la seroprevalencia de Esta enfermedad va diseminándose a los Neosporosis Bovina y su efecto sobre el intervalo establecimientos, los factores que podrían haber entre partos en el año 2018. facilitado la difusión de N. caninum serían; la permanencia de vacas seropositivas (transmisión vertical) dentro del hato, presencia de caninos que Finalizado el trabajo de campo y obtenido los conviven con los bovinos dentro de los establos y resultados de laboratorio, se realizó el análisis de los falta de medidas higiénicas sanitarias en las datos recabados, y el mismo arrojo los siguientes explotaciones lecheras (12). Un factor importante resultados: de un total de 87 muestras analizadas se sería también la no exigencia de pruebas que obtuvieron 36 muestras positivas que representa el certifiquen la seronegatividad de los animales 41,4% y 51 muestras resultaron negativas, incorporados al rebaño.…”

Section: De 31 L a B O R A T O R I O D E S E N A C S A E N T U B O S D Eunclassified

Seroprevalence of Neosporosis and Its Effect on the Interval Between Births in Dairy Cows of the Central Department, Paraguay - Year 2018

Giménez

Alonso

Vera

et al. 2019

Compend. cienc. vet

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The objective of this research was to evaluate the seroprevalence of Neosporosis in dairy cows and its effect on the interval between births, the same was carried out in the districts of San Lorenzo, Ñemby and Nueva Italia of the Central Department during the year 2018. There were selected 87 animals to wich blood samples were taken from the medial coccygeal vein, which were deposited in test tubes without anticoagulant for subsequent centrifugation, the serum obtained from each sample in microcentrifuge tubes, were sent refrigerated to the SENACSA ? ? Laboratory, where they were studied using the indirect ELISA method for diagnosis, finding the following results: 41.4% (36 samples) were positive. In the evaluation of the interval between births it was found that there is a statistically significant difference (p = 0.01) between the seropositive animals with an average (± SD) of 546 days (± 153) and the seronegative with an average (± SD) of 463 days (± 119). In all the sampled establishments, a total of 13 abortions were registered, of which 10 animals were seropositive to Neospora caninum.

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SEROPREVALENCIA DE Neospora caninum EN BOVINOS LECHEROS DE CUATRO DISTRITOS DEL VALLE DEL MANTARO, JUNÍN.

Cited by 7 publications

References 11 publications

Evaluation of abortions spontaneously induced by Neospora caninum and risk factors in dairy cattle from Lima, Peru

Evaluation of abortions spontaneously induced by Neospora caninum and risk factors in dairy cattle from Lima, Peru

Selection of Polymorphic Patterns Obtained by RAPD-PCR through Qualitative and Quantitative Analyses to Differentiate Aspergillus fumigatus

Seroprevalence of Neosporosis and Its Effect on the Interval Between Births in Dairy Cows of the Central Department, Paraguay - Year 2018

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