Seroprevalensi Penyakit Avian Influenza Subtipe H5n1  Pada Ayam Buras Di Pasar Beringkit Dan Galiran, Bali

Pertiwi, Baiq Indah; Kencana, Gusti Ayu Yuniati; Suartha, I Nyoman

doi:10.22146/jsv.55896

Cited by 3 publications

(5 citation statements)

References 6 publications

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“…Penyebaran wabah ini berlangsung sangat cepat. Menurut Pratiwi et al (2020) virus AI masih endemis di Bali, dan bersirkulasi di pasar unggas. Pasar unggas merupakan salah satu tempat yang berperan penting dalam pelestarian, perbanyakan, dan penyebaran virus AI dari unggas ke unggas lain serta dari unggas ke manusia (Leung et al, 2007;Dinh et al, 2007).…”

Section: Pendahuluanunclassified

Histopatologi Limpa Ayam Petelur Pascavaksinasi Avian Influenza H5N1 Isolat dari Bali

Wardani

Kencana

Winaya

et al. 2022

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Avian Influenza H5N1 termasuk ke dalam kelompok penyakit menular strategis dan bersifat zoonosis mematikan baik pada hewan maupun manusia yang terinfeksi. Salah satu strategi utama dalam pencegahan AI di Indonesia yaitu dengan vaksinasi. Efektivitas vaksin AI akan lebih baik apabila strain virus dalam vaksin yang digunakan homolog dengan strain virus yang ada di lapangan. Penelitian ini bertujuan untuk mengetahui histopatologi limpa ayam petelur pascavaksinasi AI-H5N1 isolat dari Bali dengan parameter diameter folikel limfoid pulpa putih limpa. Sebanyak 36 ekor ayam petelur strain Novogen Brown digunakan untuk sampel penelitian. Sampel dibagi menjadi 2 kelompok yaitu P0/kelompok kontrol tanpa perlakuan dan P1 kelompok dengan perlakuan vaksinasi AI-H5N1 isolat dari Bali. Masing-masing kelompok terdiri dari 18 ekor ayam petelur. Ayam dipelihara sejak berusia 1 hari. Vaksinasi dilakukan setelah ayam berumur 5 minggu dan di booster pada umur 10 minggu dengan dosis vaksin 0,5 ml/ekor. Pengambilan sampel organ limpa dilakukan tiga minggu pascavaksinasi booster untuk pembuatan preparat histopatologi. Data hasil pemeriksaan preparat histopatologi limpa ayam petelur dianalisis dengan uji-t. Hasil penelitian pengukuran pada organ limpa, menunjukkan rerata diameter folikel limfoid pulpa putih limpa ayam petelur dengan perlakuan vaksinasi AI-H5N1 isolat dari Bali memiliki diameter lebih besar dibandingkan dengan kelompok ayam yang tidak divaksinasi. Simpulan dari penelitian ini adalah vaksinasi AI-H5N1 isolat Bali berpengaruh nyata terhadap penambahan diameter folikel limfoid pulpa putih limpa ayam petelur pascavaksinasi.

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Section: Pendahuluanunclassified

Histopatologi Limpa Ayam Petelur Pascavaksinasi Avian Influenza H5N1 Isolat dari Bali

Wardani

Kencana

Winaya

et al. 2022

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“…Peternakan ayam petelur memiliki kendala berbagai penyakit salah satunya yaitu Avian Influenza (AI). Pertiwi et al, (2020) menyatakan bahwa virus AI masih endemis di Bali. Keganasan virus AI dapat dibedakan menjadi dua, yaitu low pathogenic avian influenza (LPAI) yang menyebabkan penyakit ringan dan highly pathogenic avian influenza (HPAI) yang menyebabkan penyakit ganas contohnya subtipe H5N1 (Kencana, 2012;Swayne and Swarez, 2000).…”

Section: Pendahuluanunclassified

“…Vaksin yang baik adalah vaksin yang memiliki homologi genetik dan antigenik yang mendekati sempurna dengan virus yang beredar di wilayah yang bersangkutan (Mahardika et al, 2009). Kencana et al, (2020) menyatakan bahwa seed vaksin isolat virus AI subtipe H5N1 asal Bali (A/Chicken/Bali9C/GAY/2019) termasuk clade 2.3.2, memiliki kekerabatan 96,72% dengan seed vaksin pemerintah Indonesia A/duck/ Sukoharjo/BBVW-1428-9/2012. Kandungan virus vaksin AI-H5N1 isolat dari Bali adalah sebesar 10 6,9 ELD50 dan telah lolos uji seed dari PT Sanbio Laboratories, Bogor (Kencana et al, 2020).…”

Section: Pendahuluanunclassified

“…Kencana et al, (2020) menyatakan bahwa seed vaksin isolat virus AI subtipe H5N1 asal Bali (A/Chicken/Bali9C/GAY/2019) termasuk clade 2.3.2, memiliki kekerabatan 96,72% dengan seed vaksin pemerintah Indonesia A/duck/ Sukoharjo/BBVW-1428-9/2012. Kandungan virus vaksin AI-H5N1 isolat dari Bali adalah sebesar 10 6,9 ELD50 dan telah lolos uji seed dari PT Sanbio Laboratories, Bogor (Kencana et al, 2020). Uji laboratorium telah dilakukan di BSL-3 PT Sanbio Laboratories, dan saat ini sedang dilakukan uji lapang.…”

Section: Pendahuluanunclassified

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Respon Imun Seluler Ayam Petelur Pascavaksinasi Avian Influenza Subtipe H5N1 Isolat dari Bali

Kencana

Sari

Suartha

et al. 2021

Jurnal Sain Veteriner

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Avian Influenza subtype H5N1 (AI-H5N1) is a malignant virus that is very detrimental to laying chickens because it is highly contagious and mutates easily. Prevention of AI-H5N1 disease in laying chickens is carried out by vaccination, therefore to maintain the quality of the vaccine, continuous research is needed. This study aims to determine the potential of AI-H5N1 vaccine isolates from Bali as measured based on cellular immune response based on total and differential leukocyte cells. Formation of antibodies is influenced by the nonspecific and specific immune system involving leukocytes, especially lymphocytes. Total of 40 layers of Novogen Brown strain were used for the research sample, kept since the age of one day on a commercial farm in Perean Village, Tabanan Regency, Bali. Laying chickens are vaccinated at 5 weeks of age by intramuscular injection. Total of 20 chikens were taken randomly and used for the sample. Blood draws were performed once pre-vaccination and five times each week after vaccination with anticoagulants. Total leukocytes were examined by an auto hematology analyzer, while differential leucocytes with thin blood smear stained with Giemsa. Total and differential leukocyte data were analyzed by means of the variance test followed by the Duncan test. Results showed that AI-H5N1 vaccination from Bali isolates could increase total and differential leucocytes of laying chickens and had significant effect on the mean total leukocytes, the absolute values of heterophyll cells, eosinophils, lymphocytes, and monocytes, but had no significant effect on post-vaccination basophil cells.

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“…Kata kunci: Ayam broiler; Avian Influenza PENDAHULUAN Penyakit Avian Influenza belum bisa diberantas di Indonesia dan penyakit ini masih endemis di Bali (Pratiwi et al, 2020). Sejak tahun 2002, virus AI menyebar hampir ke seluruh bagian dunia (Alexander, 2007) termasuk Indonesia (Kandun et al, 2006).…”

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Case of Avian Influenza in Broiler Chicken at Jatiluwih Village

Gayanti,

Mahardika,

Winaya

et al. 2024

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Avian Influenza (AI) is still endemic in Bali. This disease is very dangerous and deadly, is zoonotic in birds and humans and causes high economic losses. AI disease in poultry is caused by the Influenza virus type A. The aim of the examination is to identify the agent that caused the death of the chicken in the case to determine a definite diagnosis. The case chicken sample was a 29 day old broiler chicken from a farm in Jatiluwih Village, Tabanan Regency, Bali. Clinical symptoms of chickens include: weak chickens, shaking, shortness of breath, no appetite for eating or drinking, dull feathers, pale bluish combs, runny discharge from the beak, and white-brown watery feces. Chicken death occurred 2 days after clinical symptoms appeared. After the chicken dies, a necropsy is carried out and the samples are examined in the histopathology, virology, bacteriology and parasitology laboratories to determine the agent that caused the death. Histopathological examination showed that all organs had lesions. The results of the HA/HI test showed that the chicken was a positive case of being infected with the Avian Influenza virus. Bacterial infection testing in the media test, selective test, primary test, secondary test and confectionery test identified the presence of Staphylococcus sp bacteria. in the liver and lungs. The results of fecal examination during parasite examination using native and concentration methods did not reveal any worm eggs or protozoa. It was concluded that the case chicken was infected with Avian Influenza with secondary bacterial infection, namely Staphylococcus sp. It is recommended that breeders improve biosecurity and carry out routine and appropriate vaccinations to prevent Avian Influenza disease.

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Seroprevalensi Penyakit Avian Influenza Subtipe H5n1 Pada Ayam Buras Di Pasar Beringkit Dan Galiran, Bali

Cited by 3 publications

References 6 publications

Histopatologi Limpa Ayam Petelur Pascavaksinasi Avian Influenza H5N1 Isolat dari Bali

Histopatologi Limpa Ayam Petelur Pascavaksinasi Avian Influenza H5N1 Isolat dari Bali

Respon Imun Seluler Ayam Petelur Pascavaksinasi Avian Influenza Subtipe H5N1 Isolat dari Bali

Case of Avian Influenza in Broiler Chicken at Jatiluwih Village

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