Seroreactivity to Helicobacter pylori Antigens as a Risk Indicator of Gastric Cancer

Abstract

Background: Multiple etiologic factors are suspected to cause gastric cancer, the most important of which is infection with virulent types of Helicobacter pylori. Materials and Methods: We have compared 102 gastric cancer patients with 122 non-ulcer, non-cancer dyspeptic patients. Gastric specimens were evaluated for H. pylori infection by tissue-based detection methods. Patient sera underwent antigen-specific ELISA and western blotting using a Helicoblot 2.1 kit and antibody responses to various H. pylori antigens were assessed. Results: The absolute majority (97-100%) of both groups were H. pylori seropositive. Multivariate regression analysis demonstrated serum antibodies to the low molecular weight 35kDa protein to be protective and reduce the risk of gastric cancer by 60% (OR:0.4; 95%CI:0.1-0.9). Conversely, seroreactivity to the 89kDa (VacA) protein was significantly higher in gastric cancer patients (OR:2.7; 95%CI:1.0-7.1). There was a highly significant association (p<0.001) between seroreactivity to the 116kDa (CagA) and 89kDa (VacA) proteins, and double positive subjects were found at nearly five fold (OR:4.9; 95%CI:1.0-24.4) enhanced risk of gastric cancer as compared to double negative subjects. Conclusions: Seroreactivity to H. pylori low (35kDa) and high (116kDa/89kDa) molecular weight antigens were respectively revealed as protective and risk indicators for gastric cancer.

Clinical Characteristics and Helicobacter pylori Status of Gastric Cancer in Thailand

“…The prevalence of H. pylori infection was high among gastric cancer patients in many countries such as 91% in Singapore (Chau et al, 2002), 78% in Japan (Ono et al, 2012) and 100% in Iran (Karami et al, 2013). In this study, we demonstrated that overall H. pylori infection in gastric cancer was 83%.…”

“…Since Warren and Marshall first isolated H. pylori and found its association with gastritis (Warren et al, 1983), many studies have suggested the relationship between H. pylori infection and gastric cancer (Parsonnet et al, 1991, Uemura et al, 2001, Demirel et al, 2013, Karami et al, 2013. In this study, the prevalence of active and non-active H. pylori infection in gastric cancer was 40% and 43% respectively.…”

“…The pathogenesis of H. pylori in the development of gastric cancer has been suggested primarily from both epidemiological and basic research studies (Parsonnet et al, 1991, Uemura et al, 2001, Demirel et al, 2013, Karami et al, 2013, Basiri et al, 2014. The World Health Organization and International Agency for Research on Cancer consensus group stated in 1994 based on epidemiologic and histological evidence has classified H. pylori as a definite class I carcinogen (IARC 1994;Abeba et al, 2014).…”

Helicobacter pylori Infection Impacts on Functional Dyspepsia in Thailand

“…Furthermore, those with H. pylori infection will get chance to develop gastric cancer between three to six times more than uninfected persons (Forman et al, 1991;Nomura et al, 1991;Parsonnet et al, 1991). Seroreactivity to the 89kDa (VacA) and 116kDa (CagA) proteins in H. pylori infected patients is risk indicators for cancer of stomach (Karami et al, 2013;Basiri et al, 2014). H. pylori eradication is not only significantly improve symptoms of FD patients (Moayyedi et al, 2006) but also decreases the gastric cancer incidence in asymptomatic infected Asian persons (Hamajima et al, 2004;Ford et al, 2014).…”

Prevalence and Risk Factors of H. pylori from Dyspeptic Patients in Northwest Ethiopia: A Hospital Based Cross-sectional Study

“…High risk individuals should be monitored for early signs of neoplastic changes and advised for possible life style changes (Karami et al, 2013). Some studies were conducted on the seroprevalence and risk factors of H. pylori infection from dyspeptic patients in Ethiopia (Desta et al, 2002;Asrat et al, 2004;Tadege et al, 2005;Moges et al, 2006).…”